CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/041.mrc Output diagnostic file name Micrographs_0601/041.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/041.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2190.000 7524.000 Mean, RMS : 4435.042 223.6164 TITLE 1: emi2mrc: 041.emi Sun Jan 05 14:00:05 2014 TITLE 2: kV 300, Mag 78000x, Def 24120, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 145.536, Y 306.872, Z -103.054, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01529 16400.00 10000.00 0.00 -0.01499 16600.00 10000.00 0.00 -0.01455 16800.00 10000.00 0.00 -0.01411 17000.00 10000.00 0.00 -0.01352 17200.00 10000.00 0.00 -0.01289 17400.00 10000.00 0.00 -0.01234 17600.00 10000.00 0.00 -0.01185 17800.00 10000.00 0.00 -0.01126 18000.00 10000.00 0.00 -0.01052 18200.00 10000.00 0.00 -0.00978 18400.00 10000.00 0.00 -0.00905 18600.00 10000.00 0.00 -0.00834 18800.00 10000.00 0.00 -0.00777 19000.00 10000.00 0.00 -0.00708 19200.00 10000.00 0.00 -0.00620 19400.00 10000.00 0.00 -0.00544 19600.00 10000.00 0.00 -0.00470 19800.00 10000.00 0.00 -0.00382 20000.00 10000.00 0.00 -0.00306 20200.00 10000.00 0.00 -0.00241 20400.00 10000.00 0.00 -0.00183 20600.00 10000.00 0.00 -0.00123 20800.00 10000.00 0.00 -0.00044 21000.00 10000.00 0.00 0.00039 21200.00 10000.00 0.00 0.00120 21400.00 10000.00 0.00 0.00205 21600.00 10000.00 0.00 0.00286 21800.00 10000.00 0.00 0.00366 22000.00 10000.00 0.00 0.00465 22200.00 10000.00 0.00 0.00574 22400.00 10000.00 0.00 0.00666 22600.00 10000.00 0.00 0.00768 22800.00 10000.00 0.00 0.00876 23000.00 10000.00 0.00 0.00962 23200.00 10000.00 0.00 0.01060 23400.00 10000.00 0.00 0.01180 23600.00 10000.00 0.00 0.01314 23800.00 10000.00 0.00 0.01463 24000.00 10000.00 0.00 0.01594 24200.00 10000.00 0.00 0.01715 24400.00 10000.00 0.00 0.01836 24600.00 10000.00 0.00 0.01971 24800.00 10000.00 0.00 0.02143 25000.00 10000.00 0.00 0.02331 25200.00 10000.00 0.00 0.02510 25400.00 10000.00 0.00 0.02645 25600.00 10000.00 0.00 0.02725 25800.00 10000.00 0.00 0.02774 26000.00 10000.00 0.00 0.02798 26200.00 10000.00 0.00 0.02806 25800.00 10200.00 0.00 0.02811 26000.00 10200.00 0.00 0.02833 26200.00 10200.00 0.00 0.02841 26000.00 10400.00 0.00 0.02851 26200.00 10400.00 0.00 0.02862 26000.00 10600.00 0.00 0.02889 26200.00 10600.00 0.00 0.02908 25800.00 10800.00 0.00 0.02915 26000.00 10800.00 0.00 0.02954 26200.00 10800.00 0.00 0.02971 26000.00 11000.00 0.00 0.03001 26200.00 11000.00 0.00 0.03006 26000.00 11200.00 0.00 0.03031 25800.00 11400.00 0.00 0.03037 26000.00 11400.00 0.00 0.03061 26200.00 11400.00 0.00 0.03064 25800.00 11600.00 0.00 0.03076 26000.00 11600.00 0.00 0.03105 26200.00 11600.00 0.00 0.03115 26400.00 11600.00 0.00 0.03118 25800.00 11800.00 0.00 0.03143 26000.00 11800.00 0.00 0.03178 26200.00 11800.00 0.00 0.03192 26400.00 11800.00 0.00 0.03193 25800.00 12000.00 0.00 0.03198 26000.00 12000.00 0.00 0.03233 26200.00 12000.00 0.00 0.03244 26000.00 12200.00 0.00 0.03274 26200.00 12200.00 0.00 0.03282 25800.00 12400.00 0.00 0.03301 26000.00 12400.00 0.00 0.03334 26200.00 12400.00 0.00 0.03338 25800.00 12600.00 0.00 0.03359 26000.00 12600.00 0.00 0.03386 25800.00 12800.00 0.00 0.03413 26000.00 12800.00 0.00 0.03436 25800.00 13000.00 0.00 0.03467 26000.00 13000.00 0.00 0.03485 25800.00 13200.00 0.00 0.03521 26000.00 13200.00 0.00 0.03533 25800.00 13400.00 0.00 0.03580 26000.00 13400.00 0.00 0.03592 26200.00 13400.00 0.00 0.03592 25800.00 13600.00 0.00 0.03647 26000.00 13600.00 0.00 0.03661 26200.00 13600.00 0.00 0.03666 25800.00 13800.00 0.00 0.03707 26000.00 13800.00 0.00 0.03722 26200.00 13800.00 0.00 0.03730 25800.00 14000.00 0.00 0.03760 26000.00 14000.00 0.00 0.03779 26200.00 14000.00 0.00 0.03791 25800.00 14200.00 0.00 0.03826 26000.00 14200.00 0.00 0.03852 26200.00 14200.00 0.00 0.03866 25800.00 14400.00 0.00 0.03900 26000.00 14400.00 0.00 0.03927 26200.00 14400.00 0.00 0.03934 25800.00 14600.00 0.00 0.03965 26000.00 14600.00 0.00 0.03988 25800.00 14800.00 0.00 0.04012 26000.00 14800.00 0.00 0.04030 25800.00 15000.00 0.00 0.04071 26000.00 15000.00 0.00 0.04085 25600.00 15200.00 0.00 0.04089 25800.00 15200.00 0.00 0.04137 26000.00 15200.00 0.00 0.04146 25600.00 15400.00 0.00 0.04155 25800.00 15400.00 0.00 0.04198 26000.00 15400.00 0.00 0.04203 25600.00 15600.00 0.00 0.04232 25800.00 15600.00 0.00 0.04273 26000.00 15600.00 0.00 0.04279 25600.00 15800.00 0.00 0.04306 25800.00 15800.00 0.00 0.04346 26000.00 15800.00 0.00 0.04352 25600.00 16000.00 0.00 0.04397 25800.00 16000.00 0.00 0.04436 26000.00 16000.00 0.00 0.04441 25600.00 16200.00 0.00 0.04493 25800.00 16200.00 0.00 0.04531 26000.00 16200.00 0.00 0.04536 25600.00 16400.00 0.00 0.04573 25800.00 16400.00 0.00 0.04612 26000.00 16400.00 0.00 0.04616 25600.00 16600.00 0.00 0.04662 25800.00 16600.00 0.00 0.04702 26000.00 16600.00 0.00 0.04706 25600.00 16800.00 0.00 0.04755 25800.00 16800.00 0.00 0.04793 26000.00 16800.00 0.00 0.04794 25600.00 17000.00 0.00 0.04836 25800.00 17000.00 0.00 0.04872 26000.00 17000.00 0.00 0.04873 25600.00 17200.00 0.00 0.04927 25800.00 17200.00 0.00 0.04963 26000.00 17200.00 0.00 0.04968 25600.00 17400.00 0.00 0.05030 25800.00 17400.00 0.00 0.05066 26000.00 17400.00 0.00 0.05070 25600.00 17600.00 0.00 0.05122 25800.00 17600.00 0.00 0.05158 26000.00 17600.00 0.00 0.05162 25600.00 17800.00 0.00 0.05223 25800.00 17800.00 0.00 0.05262 26000.00 17800.00 0.00 0.05269 25600.00 18000.00 0.00 0.05340 25800.00 18000.00 0.00 0.05382 26000.00 18000.00 0.00 0.05393 25600.00 18200.00 0.00 0.05464 25800.00 18200.00 0.00 0.05510 26000.00 18200.00 0.00 0.05522 25600.00 18400.00 0.00 0.05590 25800.00 18400.00 0.00 0.05638 26000.00 18400.00 0.00 0.05649 25600.00 18600.00 0.00 0.05721 25800.00 18600.00 0.00 0.05772 26000.00 18600.00 0.00 0.05781 25600.00 18800.00 0.00 0.05859 25800.00 18800.00 0.00 0.05911 26000.00 18800.00 0.00 0.05918 25600.00 19000.00 0.00 0.05997 25800.00 19000.00 0.00 0.06051 26000.00 19000.00 0.00 0.06055 25600.00 19200.00 0.00 0.06161 25800.00 19200.00 0.00 0.06213 25600.00 19400.00 0.00 0.06313 25800.00 19400.00 0.00 0.06356 25600.00 19600.00 0.00 0.06453 25800.00 19600.00 0.00 0.06488 25400.00 19800.00 0.00 0.06499 25600.00 19800.00 0.00 0.06598 25800.00 19800.00 0.00 0.06626 25400.00 20000.00 0.00 0.06636 25600.00 20000.00 0.00 0.06727 25800.00 20000.00 0.00 0.06748 25400.00 20200.00 0.00 0.06768 25600.00 20200.00 0.00 0.06854 25800.00 20200.00 0.00 0.06872 25400.00 20400.00 0.00 0.06895 25600.00 20400.00 0.00 0.06973 25800.00 20400.00 0.00 0.06989 25400.00 20600.00 0.00 0.07016 25600.00 20600.00 0.00 0.07086 25800.00 20600.00 0.00 0.07103 25400.00 20800.00 0.00 0.07126 25600.00 20800.00 0.00 0.07198 25800.00 20800.00 0.00 0.07219 25400.00 21000.00 0.00 0.07246 25600.00 21000.00 0.00 0.07329 25800.00 21000.00 0.00 0.07354 25400.00 21200.00 0.00 0.07402 25600.00 21200.00 0.00 0.07492 25800.00 21200.00 0.00 0.07515 25400.00 21400.00 0.00 0.07565 25600.00 21400.00 0.00 0.07655 25800.00 21400.00 0.00 0.07668 25400.00 21600.00 0.00 0.07709 25600.00 21600.00 0.00 0.07796 25800.00 21600.00 0.00 0.07803 25400.00 21800.00 0.00 0.07857 25600.00 21800.00 0.00 0.07943 25800.00 21800.00 0.00 0.07948 25400.00 22000.00 0.00 0.08028 25600.00 22000.00 0.00 0.08110 25800.00 22000.00 0.00 0.08111 25400.00 22200.00 0.00 0.08204 25600.00 22200.00 0.00 0.08281 25400.00 22400.00 0.00 0.08399 25600.00 22400.00 0.00 0.08472 25400.00 22600.00 0.00 0.08604 25600.00 22600.00 0.00 0.08665 25400.00 22800.00 0.00 0.08789 25600.00 22800.00 0.00 0.08837 25400.00 23000.00 0.00 0.08966 25600.00 23000.00 0.00 0.09015 25200.00 23200.00 0.00 0.09029 25400.00 23200.00 0.00 0.09175 25600.00 23200.00 0.00 0.09227 25200.00 23400.00 0.00 0.09246 25400.00 23400.00 0.00 0.09392 25600.00 23400.00 0.00 0.09436 25200.00 23600.00 0.00 0.09454 25400.00 23600.00 0.00 0.09593 25600.00 23600.00 0.00 0.09623 25200.00 23800.00 0.00 0.09672 25400.00 23800.00 0.00 0.09800 25600.00 23800.00 0.00 0.09815 25200.00 24000.00 0.00 0.09885 25400.00 24000.00 0.00 0.10001 25600.00 24000.00 0.00 0.10002 25200.00 24200.00 0.00 0.10101 25400.00 24200.00 0.00 0.10204 25200.00 24400.00 0.00 0.10303 25400.00 24400.00 0.00 0.10392 25200.00 24600.00 0.00 0.10509 25400.00 24600.00 0.00 0.10582 25200.00 24800.00 0.00 0.10697 25400.00 24800.00 0.00 0.10747 25200.00 25000.00 0.00 0.10853 25400.00 25000.00 0.00 0.10873 25200.00 25200.00 0.00 0.10963 25200.00 25400.00 30.00 0.10969 25200.00 25400.00 35.00 0.10976 25000.00 25400.00 40.00 0.10977 25200.00 25400.00 40.00 0.10980 25000.00 25400.00 45.00 0.10982 25200.00 25400.00 45.00 0.10983 25000.00 25400.00 50.00 0.10983 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 25127.32 25337.83 50.00 0.11007 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/041.ctf NX, NY, NZ: 512 512 1 MODE : real