CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/040.mrc Output diagnostic file name Micrographs_0601/040.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/040.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2428.000 9970.000 Mean, RMS : 4542.271 223.0104 TITLE 1: emi2mrc: 040.emi Sun Jan 05 12:34:05 2014 TITLE 2: kV 300, Mag 78000x, Def 29040, Spot 4, Int 55.10%, Obj 88.04%, Dif 73.67% TITLE 3: X 339.097, Y 277.628, Z -104.822, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00630 12400.00 10000.00 0.00 -0.00623 12600.00 10000.00 0.00 -0.00604 12800.00 10000.00 0.00 -0.00586 13000.00 10000.00 0.00 -0.00571 13200.00 10000.00 0.00 -0.00558 13400.00 10000.00 0.00 -0.00543 13600.00 10000.00 0.00 -0.00521 13800.00 10000.00 0.00 -0.00500 14000.00 10000.00 0.00 -0.00482 14200.00 10000.00 0.00 -0.00466 14400.00 10000.00 0.00 -0.00457 14600.00 10000.00 0.00 -0.00438 14800.00 10000.00 0.00 -0.00405 15000.00 10000.00 0.00 -0.00371 15200.00 10000.00 0.00 -0.00325 15400.00 10000.00 0.00 -0.00266 15600.00 10000.00 0.00 -0.00216 15800.00 10000.00 0.00 -0.00161 16000.00 10000.00 0.00 -0.00102 16200.00 10000.00 0.00 -0.00045 16400.00 10000.00 0.00 0.00024 16600.00 10000.00 0.00 0.00087 16800.00 10000.00 0.00 0.00146 17000.00 10000.00 0.00 0.00224 17200.00 10000.00 0.00 0.00299 17400.00 10000.00 0.00 0.00363 17600.00 10000.00 0.00 0.00418 17800.00 10000.00 0.00 0.00481 18000.00 10000.00 0.00 0.00554 18200.00 10000.00 0.00 0.00627 18400.00 10000.00 0.00 0.00707 18600.00 10000.00 0.00 0.00794 18800.00 10000.00 0.00 0.00897 19000.00 10000.00 0.00 0.01007 19200.00 10000.00 0.00 0.01118 19400.00 10000.00 0.00 0.01234 19600.00 10000.00 0.00 0.01335 19800.00 10000.00 0.00 0.01432 20000.00 10000.00 0.00 0.01532 20200.00 10000.00 0.00 0.01629 20400.00 10000.00 0.00 0.01727 20600.00 10000.00 0.00 0.01826 20800.00 10000.00 0.00 0.01932 21000.00 10000.00 0.00 0.02048 21200.00 10000.00 0.00 0.02162 21400.00 10000.00 0.00 0.02275 21600.00 10000.00 0.00 0.02412 21800.00 10000.00 0.00 0.02560 22000.00 10000.00 0.00 0.02701 22200.00 10000.00 0.00 0.02843 22400.00 10000.00 0.00 0.02989 22600.00 10000.00 0.00 0.03133 22800.00 10000.00 0.00 0.03293 23000.00 10000.00 0.00 0.03474 23200.00 10000.00 0.00 0.03657 23400.00 10000.00 0.00 0.03845 23600.00 10000.00 0.00 0.04033 23800.00 10000.00 0.00 0.04190 24000.00 10000.00 0.00 0.04297 24200.00 10000.00 0.00 0.04360 24400.00 10000.00 0.00 0.04396 24600.00 10000.00 0.00 0.04417 24800.00 10000.00 0.00 0.04421 24400.00 10200.00 0.00 0.04430 24600.00 10200.00 0.00 0.04450 24800.00 10200.00 0.00 0.04450 24400.00 10400.00 0.00 0.04451 24600.00 10400.00 0.00 0.04468 24400.00 10600.00 0.00 0.04476 24600.00 10600.00 0.00 0.04489 24400.00 10800.00 0.00 0.04508 24600.00 10800.00 0.00 0.04517 24200.00 11000.00 0.00 0.04521 24400.00 11000.00 0.00 0.04549 24600.00 11000.00 0.00 0.04556 24200.00 11200.00 0.00 0.04562 24400.00 11200.00 0.00 0.04590 24600.00 11200.00 0.00 0.04598 24200.00 11400.00 0.00 0.04605 24400.00 11400.00 0.00 0.04630 24600.00 11400.00 0.00 0.04638 24800.00 11400.00 0.00 0.04639 24200.00 11600.00 0.00 0.04639 24400.00 11600.00 0.00 0.04662 24600.00 11600.00 0.00 0.04671 24800.00 11600.00 0.00 0.04677 25000.00 11600.00 0.00 0.04679 24400.00 11800.00 0.00 0.04696 24600.00 11800.00 0.00 0.04712 24800.00 11800.00 0.00 0.04725 25000.00 11800.00 0.00 0.04727 24400.00 12000.00 0.00 0.04741 24600.00 12000.00 0.00 0.04764 24800.00 12000.00 0.00 0.04778 24400.00 12200.00 0.00 0.04800 24600.00 12200.00 0.00 0.04825 24800.00 12200.00 0.00 0.04836 24200.00 12400.00 0.00 0.04840 24400.00 12400.00 0.00 0.04875 24600.00 12400.00 0.00 0.04899 24800.00 12400.00 0.00 0.04907 24200.00 12600.00 0.00 0.04909 24400.00 12600.00 0.00 0.04943 24600.00 12600.00 0.00 0.04967 24800.00 12600.00 0.00 0.04975 24200.00 12800.00 0.00 0.04980 24400.00 12800.00 0.00 0.05017 24600.00 12800.00 0.00 0.05043 24800.00 12800.00 0.00 0.05049 24200.00 13000.00 0.00 0.05058 24400.00 13000.00 0.00 0.05098 24600.00 13000.00 0.00 0.05122 24800.00 13000.00 0.00 0.05122 24200.00 13200.00 0.00 0.05138 24400.00 13200.00 0.00 0.05174 24600.00 13200.00 0.00 0.05190 24200.00 13400.00 0.00 0.05205 24400.00 13400.00 0.00 0.05235 24600.00 13400.00 0.00 0.05248 24200.00 13600.00 0.00 0.05267 24400.00 13600.00 0.00 0.05297 24600.00 13600.00 0.00 0.05314 24200.00 13800.00 0.00 0.05351 24400.00 13800.00 0.00 0.05382 24600.00 13800.00 0.00 0.05398 24200.00 14000.00 0.00 0.05439 24400.00 14000.00 0.00 0.05466 24600.00 14000.00 0.00 0.05477 24200.00 14200.00 0.00 0.05513 24400.00 14200.00 0.00 0.05537 24600.00 14200.00 0.00 0.05546 24200.00 14400.00 0.00 0.05592 24400.00 14400.00 0.00 0.05614 24600.00 14400.00 0.00 0.05625 24200.00 14600.00 0.00 0.05667 24400.00 14600.00 0.00 0.05689 24600.00 14600.00 0.00 0.05699 24200.00 14800.00 0.00 0.05728 24400.00 14800.00 0.00 0.05752 24600.00 14800.00 0.00 0.05761 24200.00 15000.00 0.00 0.05807 24400.00 15000.00 0.00 0.05834 24600.00 15000.00 0.00 0.05841 24000.00 15200.00 0.00 0.05844 24200.00 15200.00 0.00 0.05896 24400.00 15200.00 0.00 0.05923 24600.00 15200.00 0.00 0.05926 24000.00 15400.00 0.00 0.05945 24200.00 15400.00 0.00 0.05997 24400.00 15400.00 0.00 0.06019 24000.00 15600.00 0.00 0.06050 24200.00 15600.00 0.00 0.06098 24400.00 15600.00 0.00 0.06116 24000.00 15800.00 0.00 0.06152 24200.00 15800.00 0.00 0.06199 24400.00 15800.00 0.00 0.06219 24000.00 16000.00 0.00 0.06276 24200.00 16000.00 0.00 0.06322 24400.00 16000.00 0.00 0.06342 24000.00 16200.00 0.00 0.06378 24200.00 16200.00 0.00 0.06420 24400.00 16200.00 0.00 0.06437 24000.00 16400.00 0.00 0.06479 24200.00 16400.00 0.00 0.06520 24400.00 16400.00 0.00 0.06539 24000.00 16600.00 0.00 0.06599 24200.00 16600.00 0.00 0.06643 24400.00 16600.00 0.00 0.06664 24000.00 16800.00 0.00 0.06723 24200.00 16800.00 0.00 0.06770 24400.00 16800.00 0.00 0.06793 24000.00 17000.00 0.00 0.06853 24200.00 17000.00 0.00 0.06905 24400.00 17000.00 0.00 0.06926 24000.00 17200.00 0.00 0.06993 24200.00 17200.00 0.00 0.07043 24400.00 17200.00 0.00 0.07058 24000.00 17400.00 0.00 0.07142 24200.00 17400.00 0.00 0.07187 24400.00 17400.00 0.00 0.07196 24000.00 17600.00 0.00 0.07276 24200.00 17600.00 0.00 0.07313 24400.00 17600.00 0.00 0.07320 24000.00 17800.00 0.00 0.07401 24200.00 17800.00 0.00 0.07433 24400.00 17800.00 0.00 0.07438 24000.00 18000.00 0.00 0.07518 24200.00 18000.00 0.00 0.07549 24400.00 18000.00 0.00 0.07553 24000.00 18200.00 0.00 0.07641 24200.00 18200.00 0.00 0.07674 24400.00 18200.00 0.00 0.07678 24000.00 18400.00 0.00 0.07762 24200.00 18400.00 0.00 0.07797 24400.00 18400.00 0.00 0.07799 24000.00 18600.00 0.00 0.07886 24200.00 18600.00 0.00 0.07922 24400.00 18600.00 0.00 0.07924 24000.00 18800.00 0.00 0.08029 24200.00 18800.00 0.00 0.08067 24400.00 18800.00 0.00 0.08069 24000.00 19000.00 0.00 0.08165 24200.00 19000.00 0.00 0.08206 24000.00 19200.00 0.00 0.08329 24200.00 19200.00 0.00 0.08366 23800.00 19400.00 0.00 0.08372 24000.00 19400.00 0.00 0.08500 24200.00 19400.00 0.00 0.08524 23800.00 19600.00 0.00 0.08540 24000.00 19600.00 0.00 0.08658 24200.00 19600.00 0.00 0.08673 23800.00 19800.00 0.00 0.08698 24000.00 19800.00 0.00 0.08811 24200.00 19800.00 0.00 0.08823 23800.00 20000.00 0.00 0.08851 24000.00 20000.00 0.00 0.08960 24200.00 20000.00 0.00 0.08971 23800.00 20200.00 0.00 0.09013 24000.00 20200.00 0.00 0.09120 24200.00 20200.00 0.00 0.09131 23800.00 20400.00 0.00 0.09186 24000.00 20400.00 0.00 0.09292 24200.00 20400.00 0.00 0.09301 23800.00 20600.00 0.00 0.09371 24000.00 20600.00 0.00 0.09474 24200.00 20600.00 0.00 0.09479 23800.00 20800.00 0.00 0.09541 24000.00 20800.00 0.00 0.09639 23800.00 21000.00 0.00 0.09727 24000.00 21000.00 0.00 0.09821 23800.00 21200.00 0.00 0.09921 24000.00 21200.00 0.00 0.10011 23800.00 21400.00 0.00 0.10114 24000.00 21400.00 0.00 0.10197 23800.00 21600.00 0.00 0.10307 24000.00 21600.00 0.00 0.10384 23800.00 21800.00 0.00 0.10509 24000.00 21800.00 0.00 0.10583 23800.00 22000.00 0.00 0.10727 24000.00 22000.00 0.00 0.10797 23800.00 22200.00 0.00 0.10933 24000.00 22200.00 0.00 0.10999 23800.00 22400.00 0.00 0.11167 24000.00 22400.00 0.00 0.11228 23800.00 22600.00 0.00 0.11396 24000.00 22600.00 0.00 0.11446 23800.00 22800.00 0.00 0.11625 24000.00 22800.00 0.00 0.11664 23600.00 23000.00 0.00 0.11677 23800.00 23000.00 0.00 0.11861 24000.00 23000.00 0.00 0.11886 23600.00 23200.00 0.00 0.11914 23800.00 23200.00 0.00 0.12091 24000.00 23200.00 0.00 0.12096 23600.00 23400.00 0.00 0.12146 23800.00 23400.00 0.00 0.12313 23600.00 23600.00 0.00 0.12327 23800.00 23600.00 0.00 0.12487 23600.00 23800.00 0.00 0.12510 23800.00 23800.00 0.00 0.12633 23600.00 24000.00 15.00 0.12639 23600.00 24000.00 20.00 0.12655 23600.00 24000.00 25.00 0.12664 23600.00 24000.00 30.00 0.12666 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23701.88 23929.37 27.97 0.12694 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/040.ctf NX, NY, NZ: 512 512 1 MODE : real