CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/038.mrc Output diagnostic file name Micrographs_0601/038.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/038.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2290.000 8306.000 Mean, RMS : 4473.878 225.6694 TITLE 1: emi2mrc: 038.emi Sun Jan 05 13:58:01 2014 TITLE 2: kV 300, Mag 78000x, Def 24450, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X 148.640, Y 309.081, Z -103.054, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01019 15000.00 10000.00 0.00 -0.01015 15200.00 10000.00 0.00 -0.00976 15400.00 10000.00 0.00 -0.00922 15600.00 10000.00 0.00 -0.00867 15800.00 10000.00 0.00 -0.00811 16000.00 10000.00 0.00 -0.00751 16200.00 10000.00 0.00 -0.00691 16400.00 10000.00 0.00 -0.00642 16600.00 10000.00 0.00 -0.00599 16800.00 10000.00 0.00 -0.00537 17000.00 10000.00 0.00 -0.00469 17200.00 10000.00 0.00 -0.00414 17400.00 10000.00 0.00 -0.00359 17600.00 10000.00 0.00 -0.00293 17800.00 10000.00 0.00 -0.00212 18000.00 10000.00 0.00 -0.00125 18200.00 10000.00 0.00 -0.00048 18400.00 10000.00 0.00 0.00028 18600.00 10000.00 0.00 0.00109 18800.00 10000.00 0.00 0.00187 19000.00 10000.00 0.00 0.00266 19200.00 10000.00 0.00 0.00355 19400.00 10000.00 0.00 0.00463 19600.00 10000.00 0.00 0.00574 19800.00 10000.00 0.00 0.00688 20000.00 10000.00 0.00 0.00806 20200.00 10000.00 0.00 0.00909 20400.00 10000.00 0.00 0.01016 20600.00 10000.00 0.00 0.01130 20800.00 10000.00 0.00 0.01249 21000.00 10000.00 0.00 0.01381 21200.00 10000.00 0.00 0.01500 21400.00 10000.00 0.00 0.01607 21600.00 10000.00 0.00 0.01722 21800.00 10000.00 0.00 0.01828 22000.00 10000.00 0.00 0.01943 22200.00 10000.00 0.00 0.02076 22400.00 10000.00 0.00 0.02207 22600.00 10000.00 0.00 0.02353 22800.00 10000.00 0.00 0.02521 23000.00 10000.00 0.00 0.02694 23200.00 10000.00 0.00 0.02871 23400.00 10000.00 0.00 0.03057 23600.00 10000.00 0.00 0.03245 23800.00 10000.00 0.00 0.03438 24000.00 10000.00 0.00 0.03609 24200.00 10000.00 0.00 0.03733 24400.00 10000.00 0.00 0.03825 24600.00 10000.00 0.00 0.03879 24800.00 10000.00 0.00 0.03906 25000.00 10000.00 0.00 0.03927 25200.00 10000.00 0.00 0.03937 24800.00 10200.00 0.00 0.03943 25000.00 10200.00 0.00 0.03963 25200.00 10200.00 0.00 0.03974 24800.00 10400.00 0.00 0.03992 25000.00 10400.00 0.00 0.04016 25200.00 10400.00 0.00 0.04028 24800.00 10600.00 0.00 0.04055 25000.00 10600.00 0.00 0.04079 25200.00 10600.00 0.00 0.04089 24800.00 10800.00 0.00 0.04109 25000.00 10800.00 0.00 0.04130 25200.00 10800.00 0.00 0.04136 24800.00 11000.00 0.00 0.04153 25000.00 11000.00 0.00 0.04171 25200.00 11000.00 0.00 0.04178 24800.00 11200.00 0.00 0.04188 25000.00 11200.00 0.00 0.04205 25200.00 11200.00 0.00 0.04213 24800.00 11400.00 0.00 0.04230 25000.00 11400.00 0.00 0.04249 25200.00 11400.00 0.00 0.04259 24800.00 11600.00 0.00 0.04271 25000.00 11600.00 0.00 0.04293 25200.00 11600.00 0.00 0.04304 24800.00 11800.00 0.00 0.04321 25000.00 11800.00 0.00 0.04345 25200.00 11800.00 0.00 0.04355 24800.00 12000.00 0.00 0.04379 25000.00 12000.00 0.00 0.04402 25200.00 12000.00 0.00 0.04410 24800.00 12200.00 0.00 0.04435 25000.00 12200.00 0.00 0.04457 25200.00 12200.00 0.00 0.04465 24600.00 12400.00 0.00 0.04466 24800.00 12400.00 0.00 0.04503 25000.00 12400.00 0.00 0.04524 25200.00 12400.00 0.00 0.04530 24800.00 12600.00 0.00 0.04560 25000.00 12600.00 0.00 0.04577 25200.00 12600.00 0.00 0.04579 24800.00 12800.00 0.00 0.04607 25000.00 12800.00 0.00 0.04622 25200.00 12800.00 0.00 0.04622 24600.00 13000.00 0.00 0.04637 24800.00 13000.00 0.00 0.04668 25000.00 13000.00 0.00 0.04684 25200.00 13000.00 0.00 0.04684 24600.00 13200.00 0.00 0.04715 24800.00 13200.00 0.00 0.04745 25000.00 13200.00 0.00 0.04760 24600.00 13400.00 0.00 0.04783 24800.00 13400.00 0.00 0.04813 25000.00 13400.00 0.00 0.04827 24600.00 13600.00 0.00 0.04850 24800.00 13600.00 0.00 0.04883 25000.00 13600.00 0.00 0.04897 24600.00 13800.00 0.00 0.04931 24800.00 13800.00 0.00 0.04965 25000.00 13800.00 0.00 0.04978 24600.00 14000.00 0.00 0.05007 24800.00 14000.00 0.00 0.05040 25000.00 14000.00 0.00 0.05050 24600.00 14200.00 0.00 0.05080 24800.00 14200.00 0.00 0.05111 25000.00 14200.00 0.00 0.05115 24600.00 14400.00 0.00 0.05156 24800.00 14400.00 0.00 0.05183 25000.00 14400.00 0.00 0.05183 24400.00 14600.00 0.00 0.05194 24600.00 14600.00 0.00 0.05248 24800.00 14600.00 0.00 0.05272 24400.00 14800.00 0.00 0.05290 24600.00 14800.00 0.00 0.05343 24800.00 14800.00 0.00 0.05361 24400.00 15000.00 0.00 0.05381 24600.00 15000.00 0.00 0.05431 24800.00 15000.00 0.00 0.05446 24400.00 15200.00 0.00 0.05468 24600.00 15200.00 0.00 0.05519 24800.00 15200.00 0.00 0.05531 24400.00 15400.00 0.00 0.05566 24600.00 15400.00 0.00 0.05614 24800.00 15400.00 0.00 0.05622 24400.00 15600.00 0.00 0.05669 24600.00 15600.00 0.00 0.05709 24800.00 15600.00 0.00 0.05711 24400.00 15800.00 0.00 0.05755 24600.00 15800.00 0.00 0.05789 24800.00 15800.00 0.00 0.05791 24400.00 16000.00 0.00 0.05859 24600.00 16000.00 0.00 0.05894 24800.00 16000.00 0.00 0.05899 24400.00 16200.00 0.00 0.05968 24600.00 16200.00 0.00 0.06003 24800.00 16200.00 0.00 0.06007 24400.00 16400.00 0.00 0.06068 24600.00 16400.00 0.00 0.06100 24800.00 16400.00 0.00 0.06102 24400.00 16600.00 0.00 0.06176 24600.00 16600.00 0.00 0.06206 24800.00 16600.00 0.00 0.06210 24200.00 16800.00 0.00 0.06221 24400.00 16800.00 0.00 0.06301 24600.00 16800.00 0.00 0.06333 24800.00 16800.00 0.00 0.06341 24200.00 17000.00 0.00 0.06359 24400.00 17000.00 0.00 0.06441 24600.00 17000.00 0.00 0.06474 24800.00 17000.00 0.00 0.06481 24200.00 17200.00 0.00 0.06496 24400.00 17200.00 0.00 0.06575 24600.00 17200.00 0.00 0.06604 24800.00 17200.00 0.00 0.06607 24200.00 17400.00 0.00 0.06626 24400.00 17400.00 0.00 0.06701 24600.00 17400.00 0.00 0.06728 24800.00 17400.00 0.00 0.06730 24200.00 17600.00 0.00 0.06748 24400.00 17600.00 0.00 0.06823 24600.00 17600.00 0.00 0.06851 24800.00 17600.00 0.00 0.06853 24200.00 17800.00 0.00 0.06884 24400.00 17800.00 0.00 0.06960 24600.00 17800.00 0.00 0.06986 24200.00 18000.00 0.00 0.07029 24400.00 18000.00 0.00 0.07102 24600.00 18000.00 0.00 0.07124 24200.00 18200.00 0.00 0.07178 24400.00 18200.00 0.00 0.07247 24600.00 18200.00 0.00 0.07268 24200.00 18400.00 0.00 0.07327 24400.00 18400.00 0.00 0.07397 24600.00 18400.00 0.00 0.07417 24200.00 18600.00 0.00 0.07480 24400.00 18600.00 0.00 0.07549 24600.00 18600.00 0.00 0.07565 24200.00 18800.00 0.00 0.07630 24400.00 18800.00 0.00 0.07697 24600.00 18800.00 0.00 0.07707 24200.00 19000.00 0.00 0.07764 24400.00 19000.00 0.00 0.07828 24600.00 19000.00 0.00 0.07833 24200.00 19200.00 0.00 0.07905 24400.00 19200.00 0.00 0.07967 24200.00 19400.00 0.00 0.08054 24400.00 19400.00 0.00 0.08111 24200.00 19600.00 0.00 0.08213 24400.00 19600.00 0.00 0.08263 24000.00 19800.00 0.00 0.08266 24200.00 19800.00 0.00 0.08375 24400.00 19800.00 0.00 0.08418 24000.00 20000.00 0.00 0.08428 24200.00 20000.00 0.00 0.08532 24400.00 20000.00 0.00 0.08571 24000.00 20200.00 0.00 0.08574 24200.00 20200.00 0.00 0.08676 24400.00 20200.00 0.00 0.08715 24000.00 20400.00 0.00 0.08724 24200.00 20400.00 0.00 0.08826 24400.00 20400.00 0.00 0.08867 24000.00 20600.00 0.00 0.08896 24200.00 20600.00 0.00 0.08999 24400.00 20600.00 0.00 0.09036 24000.00 20800.00 0.00 0.09065 24200.00 20800.00 0.00 0.09165 24400.00 20800.00 0.00 0.09198 24000.00 21000.00 0.00 0.09250 24200.00 21000.00 0.00 0.09345 24400.00 21000.00 0.00 0.09370 24000.00 21200.00 0.00 0.09426 24200.00 21200.00 0.00 0.09519 24400.00 21200.00 0.00 0.09540 24000.00 21400.00 0.00 0.09612 24200.00 21400.00 0.00 0.09705 24400.00 21400.00 0.00 0.09720 24000.00 21600.00 0.00 0.09805 24200.00 21600.00 0.00 0.09892 24400.00 21600.00 0.00 0.09898 24000.00 21800.00 0.00 0.09990 24200.00 21800.00 0.00 0.10072 24400.00 21800.00 0.00 0.10072 24000.00 22000.00 0.00 0.10197 24200.00 22000.00 0.00 0.10274 24000.00 22200.00 0.00 0.10388 24200.00 22200.00 0.00 0.10457 24000.00 22400.00 0.00 0.10579 24200.00 22400.00 0.00 0.10639 24000.00 22600.00 0.00 0.10765 24200.00 22600.00 0.00 0.10815 24000.00 22800.00 0.00 0.10948 24200.00 22800.00 0.00 0.10984 24000.00 23000.00 0.00 0.11111 24200.00 23000.00 0.00 0.11134 23800.00 23200.00 0.00 0.11145 24000.00 23200.00 0.00 0.11272 24200.00 23200.00 0.00 0.11282 23800.00 23400.00 0.00 0.11312 24000.00 23400.00 0.00 0.11426 23800.00 23600.00 0.00 0.11448 24000.00 23600.00 0.00 0.11553 23800.00 23800.00 0.00 0.11612 24000.00 23800.00 0.00 0.11694 23800.00 24000.00 0.00 0.11713 24000.00 24000.00 0.00 0.11748 24000.00 24200.00 5.00 0.11750 23800.00 24200.00 10.00 0.11759 23800.00 24200.00 15.00 0.11768 23800.00 24200.00 20.00 0.11776 23800.00 24200.00 25.00 0.11781 23800.00 24200.00 30.00 0.11783 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23870.76 24227.94 28.61 0.11795 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/038.ctf NX, NY, NZ: 512 512 1 MODE : real