CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/037.mrc Output diagnostic file name Micrographs_0601/037.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/037.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2390.000 7706.000 Mean, RMS : 4482.551 226.2572 TITLE 1: emi2mrc: 037.emi Sun Jan 05 13:57:21 2014 TITLE 2: kV 300, Mag 78000x, Def 24450, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X 146.074, Y 309.323, Z -103.054, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00924 13600.00 10000.00 0.00 -0.00915 13800.00 10000.00 0.00 -0.00908 14400.00 10000.00 0.00 -0.00906 14600.00 10000.00 0.00 -0.00891 14800.00 10000.00 0.00 -0.00861 15000.00 10000.00 0.00 -0.00826 15200.00 10000.00 0.00 -0.00791 15400.00 10000.00 0.00 -0.00759 15600.00 10000.00 0.00 -0.00728 15800.00 10000.00 0.00 -0.00694 16000.00 10000.00 0.00 -0.00659 16200.00 10000.00 0.00 -0.00623 16400.00 10000.00 0.00 -0.00585 16600.00 10000.00 0.00 -0.00538 16800.00 10000.00 0.00 -0.00481 17000.00 10000.00 0.00 -0.00432 17200.00 10000.00 0.00 -0.00381 17400.00 10000.00 0.00 -0.00314 17600.00 10000.00 0.00 -0.00240 17800.00 10000.00 0.00 -0.00160 18000.00 10000.00 0.00 -0.00086 18200.00 10000.00 0.00 -0.00012 18400.00 10000.00 0.00 0.00076 18600.00 10000.00 0.00 0.00160 18800.00 10000.00 0.00 0.00252 19000.00 10000.00 0.00 0.00357 19200.00 10000.00 0.00 0.00449 19400.00 10000.00 0.00 0.00539 19600.00 10000.00 0.00 0.00637 19800.00 10000.00 0.00 0.00737 20000.00 10000.00 0.00 0.00832 20200.00 10000.00 0.00 0.00917 20400.00 10000.00 0.00 0.01018 20600.00 10000.00 0.00 0.01125 20800.00 10000.00 0.00 0.01215 21000.00 10000.00 0.00 0.01320 21200.00 10000.00 0.00 0.01433 21400.00 10000.00 0.00 0.01544 21600.00 10000.00 0.00 0.01663 21800.00 10000.00 0.00 0.01776 22000.00 10000.00 0.00 0.01900 22200.00 10000.00 0.00 0.02036 22400.00 10000.00 0.00 0.02171 22600.00 10000.00 0.00 0.02301 22800.00 10000.00 0.00 0.02437 23000.00 10000.00 0.00 0.02602 23200.00 10000.00 0.00 0.02780 23400.00 10000.00 0.00 0.02966 23600.00 10000.00 0.00 0.03168 23800.00 10000.00 0.00 0.03376 24000.00 10000.00 0.00 0.03561 24200.00 10000.00 0.00 0.03702 24400.00 10000.00 0.00 0.03791 24600.00 10000.00 0.00 0.03836 24800.00 10000.00 0.00 0.03863 25000.00 10000.00 0.00 0.03876 25200.00 10000.00 0.00 0.03880 24800.00 10200.00 0.00 0.03891 25000.00 10200.00 0.00 0.03901 25200.00 10200.00 0.00 0.03904 24800.00 10400.00 0.00 0.03922 25000.00 10400.00 0.00 0.03931 25200.00 10400.00 0.00 0.03935 24600.00 10600.00 0.00 0.03935 24800.00 10600.00 0.00 0.03961 25000.00 10600.00 0.00 0.03973 25200.00 10600.00 0.00 0.03977 24800.00 10800.00 0.00 0.03998 25000.00 10800.00 0.00 0.04010 24800.00 11000.00 0.00 0.04027 25000.00 11000.00 0.00 0.04034 24800.00 11200.00 0.00 0.04054 25000.00 11200.00 0.00 0.04059 24600.00 11400.00 0.00 0.04078 24800.00 11400.00 0.00 0.04105 25000.00 11400.00 0.00 0.04108 24600.00 11600.00 0.00 0.04129 24800.00 11600.00 0.00 0.04152 25000.00 11600.00 0.00 0.04153 24600.00 11800.00 0.00 0.04188 24800.00 11800.00 0.00 0.04207 24400.00 12000.00 0.00 0.04211 24600.00 12000.00 0.00 0.04258 24800.00 12000.00 0.00 0.04274 24600.00 12200.00 0.00 0.04310 24800.00 12200.00 0.00 0.04326 24400.00 12400.00 0.00 0.04326 24600.00 12400.00 0.00 0.04373 24800.00 12400.00 0.00 0.04392 25000.00 12400.00 0.00 0.04394 24400.00 12600.00 0.00 0.04405 24600.00 12600.00 0.00 0.04454 24800.00 12600.00 0.00 0.04472 24400.00 12800.00 0.00 0.04484 24600.00 12800.00 0.00 0.04530 24800.00 12800.00 0.00 0.04542 24400.00 13000.00 0.00 0.04552 24600.00 13000.00 0.00 0.04591 24800.00 13000.00 0.00 0.04597 24400.00 13200.00 0.00 0.04613 24600.00 13200.00 0.00 0.04646 24800.00 13200.00 0.00 0.04649 24400.00 13400.00 0.00 0.04664 24600.00 13400.00 0.00 0.04693 24800.00 13400.00 0.00 0.04697 24400.00 13600.00 0.00 0.04719 24600.00 13600.00 0.00 0.04749 24800.00 13600.00 0.00 0.04757 25000.00 13600.00 0.00 0.04757 24400.00 13800.00 0.00 0.04790 24600.00 13800.00 0.00 0.04821 24800.00 13800.00 0.00 0.04832 25000.00 13800.00 0.00 0.04835 24400.00 14000.00 0.00 0.04869 24600.00 14000.00 0.00 0.04901 24800.00 14000.00 0.00 0.04914 25000.00 14000.00 0.00 0.04918 24400.00 14200.00 0.00 0.04958 24600.00 14200.00 0.00 0.04989 24800.00 14200.00 0.00 0.05004 25000.00 14200.00 0.00 0.05007 24400.00 14400.00 0.00 0.05035 24600.00 14400.00 0.00 0.05066 24800.00 14400.00 0.00 0.05081 24400.00 14600.00 0.00 0.05103 24600.00 14600.00 0.00 0.05135 24800.00 14600.00 0.00 0.05151 24400.00 14800.00 0.00 0.05177 24600.00 14800.00 0.00 0.05210 24800.00 14800.00 0.00 0.05226 24400.00 15000.00 0.00 0.05254 24600.00 15000.00 0.00 0.05287 24800.00 15000.00 0.00 0.05304 24400.00 15200.00 0.00 0.05332 24600.00 15200.00 0.00 0.05368 24800.00 15200.00 0.00 0.05386 24400.00 15400.00 0.00 0.05437 24600.00 15400.00 0.00 0.05473 24800.00 15400.00 0.00 0.05491 24400.00 15600.00 0.00 0.05548 24600.00 15600.00 0.00 0.05582 24800.00 15600.00 0.00 0.05598 24400.00 15800.00 0.00 0.05647 24600.00 15800.00 0.00 0.05680 24800.00 15800.00 0.00 0.05695 24400.00 16000.00 0.00 0.05761 24600.00 16000.00 0.00 0.05793 24800.00 16000.00 0.00 0.05806 24400.00 16200.00 0.00 0.05869 24600.00 16200.00 0.00 0.05898 24800.00 16200.00 0.00 0.05909 24400.00 16400.00 0.00 0.05977 24600.00 16400.00 0.00 0.06008 24800.00 16400.00 0.00 0.06019 24400.00 16600.00 0.00 0.06084 24600.00 16600.00 0.00 0.06116 24800.00 16600.00 0.00 0.06124 24400.00 16800.00 0.00 0.06189 24600.00 16800.00 0.00 0.06220 24800.00 16800.00 0.00 0.06223 24400.00 17000.00 0.00 0.06312 24600.00 17000.00 0.00 0.06341 24800.00 17000.00 0.00 0.06345 24400.00 17200.00 0.00 0.06435 24600.00 17200.00 0.00 0.06465 24800.00 17200.00 0.00 0.06470 24200.00 17400.00 0.00 0.06475 24400.00 17400.00 0.00 0.06571 24600.00 17400.00 0.00 0.06600 24800.00 17400.00 0.00 0.06606 24200.00 17600.00 0.00 0.06622 24400.00 17600.00 0.00 0.06716 24600.00 17600.00 0.00 0.06745 24800.00 17600.00 0.00 0.06750 24200.00 17800.00 0.00 0.06768 24400.00 17800.00 0.00 0.06862 24600.00 17800.00 0.00 0.06891 24200.00 18000.00 0.00 0.06915 24400.00 18000.00 0.00 0.07007 24600.00 18000.00 0.00 0.07032 24200.00 18200.00 0.00 0.07066 24400.00 18200.00 0.00 0.07155 24600.00 18200.00 0.00 0.07175 24200.00 18400.00 0.00 0.07215 24400.00 18400.00 0.00 0.07302 24600.00 18400.00 0.00 0.07320 24200.00 18600.00 0.00 0.07360 24400.00 18600.00 0.00 0.07448 24600.00 18600.00 0.00 0.07464 24200.00 18800.00 0.00 0.07511 24400.00 18800.00 0.00 0.07599 24600.00 18800.00 0.00 0.07609 24200.00 19000.00 0.00 0.07664 24400.00 19000.00 0.00 0.07747 24600.00 19000.00 0.00 0.07751 24200.00 19200.00 0.00 0.07827 24400.00 19200.00 0.00 0.07902 24200.00 19400.00 0.00 0.07989 24400.00 19400.00 0.00 0.08057 24200.00 19600.00 0.00 0.08159 24400.00 19600.00 0.00 0.08224 24200.00 19800.00 0.00 0.08329 24400.00 19800.00 0.00 0.08393 24200.00 20000.00 0.00 0.08500 24400.00 20000.00 0.00 0.08564 24200.00 20200.00 0.00 0.08672 24400.00 20200.00 0.00 0.08735 24200.00 20400.00 0.00 0.08845 24400.00 20400.00 0.00 0.08911 24200.00 20600.00 0.00 0.09044 24400.00 20600.00 0.00 0.09110 24200.00 20800.00 0.00 0.09250 24400.00 20800.00 0.00 0.09313 24000.00 21000.00 0.00 0.09328 24200.00 21000.00 0.00 0.09475 24400.00 21000.00 0.00 0.09532 24000.00 21200.00 0.00 0.09555 24200.00 21200.00 0.00 0.09700 24400.00 21200.00 0.00 0.09748 24000.00 21400.00 0.00 0.09790 24200.00 21400.00 0.00 0.09927 24400.00 21400.00 0.00 0.09963 24000.00 21600.00 0.00 0.10026 24200.00 21600.00 0.00 0.10153 24400.00 21600.00 0.00 0.10179 24000.00 21800.00 0.00 0.10259 24200.00 21800.00 0.00 0.10379 24400.00 21800.00 0.00 0.10401 24000.00 22000.00 0.00 0.10515 24200.00 22000.00 0.00 0.10630 24400.00 22000.00 0.00 0.10649 24000.00 22200.00 0.00 0.10771 24200.00 22200.00 0.00 0.10881 24400.00 22200.00 0.00 0.10892 24000.00 22400.00 0.00 0.11057 24200.00 22400.00 0.00 0.11161 24400.00 22400.00 0.00 0.11163 24000.00 22600.00 0.00 0.11357 24200.00 22600.00 0.00 0.11453 23800.00 22800.00 0.00 0.11458 24000.00 22800.00 0.00 0.11667 24200.00 22800.00 0.00 0.11748 23800.00 23000.00 0.00 0.11769 24000.00 23000.00 0.00 0.11966 24200.00 23000.00 0.00 0.12032 23800.00 23200.00 0.00 0.12082 24000.00 23200.00 0.00 0.12265 24200.00 23200.00 0.00 0.12314 23800.00 23400.00 0.00 0.12406 24000.00 23400.00 0.00 0.12572 24200.00 23400.00 0.00 0.12595 23800.00 23600.00 0.00 0.12707 24000.00 23600.00 0.00 0.12850 23800.00 23800.00 0.00 0.13003 24000.00 23800.00 0.00 0.13090 23800.00 24000.00 0.00 0.13118 24000.00 24000.00 0.00 0.13125 23800.00 24000.00 5.00 0.13134 23800.00 24000.00 10.00 0.13148 23800.00 24000.00 15.00 0.13161 23800.00 24000.00 20.00 0.13173 23800.00 24000.00 25.00 0.13182 23800.00 24000.00 30.00 0.13189 23800.00 24000.00 35.00 0.13193 23800.00 24000.00 40.00 0.13195 23800.00 24200.00 45.00 0.13195 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23814.97 24088.75 41.66 0.13213 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/037.ctf NX, NY, NZ: 512 512 1 MODE : real