CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/036.mrc Output diagnostic file name Micrographs_0601/036.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/036.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2486.000 8114.000 Mean, RMS : 4519.747 225.8174 TITLE 1: emi2mrc: 036.emi Sun Jan 05 13:56:04 2014 TITLE 2: kV 300, Mag 78000x, Def 25100, Spot 4, Int 55.10%, Obj 88.04%, Dif 73.67% TITLE 3: X 143.453, Y 309.461, Z -102.061, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00921 15400.00 10000.00 0.00 -0.00903 15600.00 10000.00 0.00 -0.00867 15800.00 10000.00 0.00 -0.00825 16000.00 10000.00 0.00 -0.00790 16200.00 10000.00 0.00 -0.00760 16400.00 10000.00 0.00 -0.00725 16600.00 10000.00 0.00 -0.00675 16800.00 10000.00 0.00 -0.00622 17000.00 10000.00 0.00 -0.00577 17200.00 10000.00 0.00 -0.00531 17400.00 10000.00 0.00 -0.00486 17600.00 10000.00 0.00 -0.00433 17800.00 10000.00 0.00 -0.00369 18000.00 10000.00 0.00 -0.00301 18200.00 10000.00 0.00 -0.00228 18400.00 10000.00 0.00 -0.00150 18600.00 10000.00 0.00 -0.00063 18800.00 10000.00 0.00 0.00028 19000.00 10000.00 0.00 0.00117 19200.00 10000.00 0.00 0.00202 19400.00 10000.00 0.00 0.00285 19600.00 10000.00 0.00 0.00372 19800.00 10000.00 0.00 0.00462 20000.00 10000.00 0.00 0.00549 20200.00 10000.00 0.00 0.00636 20400.00 10000.00 0.00 0.00723 20600.00 10000.00 0.00 0.00822 20800.00 10000.00 0.00 0.00931 21000.00 10000.00 0.00 0.01031 21200.00 10000.00 0.00 0.01132 21400.00 10000.00 0.00 0.01233 21600.00 10000.00 0.00 0.01342 21800.00 10000.00 0.00 0.01461 22000.00 10000.00 0.00 0.01580 22200.00 10000.00 0.00 0.01727 22400.00 10000.00 0.00 0.01897 22600.00 10000.00 0.00 0.02065 22800.00 10000.00 0.00 0.02249 23000.00 10000.00 0.00 0.02435 23200.00 10000.00 0.00 0.02609 23400.00 10000.00 0.00 0.02804 23600.00 10000.00 0.00 0.03013 23800.00 10000.00 0.00 0.03215 24000.00 10000.00 0.00 0.03419 24200.00 10000.00 0.00 0.03599 24400.00 10000.00 0.00 0.03720 24600.00 10000.00 0.00 0.03794 24800.00 10000.00 0.00 0.03833 25000.00 10000.00 0.00 0.03844 24800.00 10200.00 0.00 0.03868 25000.00 10200.00 0.00 0.03874 24800.00 10400.00 0.00 0.03897 24800.00 10600.00 0.00 0.03919 24800.00 10800.00 0.00 0.03945 24600.00 11000.00 0.00 0.03955 24800.00 11000.00 0.00 0.03978 24600.00 11200.00 0.00 0.03993 24800.00 11200.00 0.00 0.04017 24600.00 11400.00 0.00 0.04037 24800.00 11400.00 0.00 0.04061 24600.00 11600.00 0.00 0.04068 24800.00 11600.00 0.00 0.04089 24600.00 11800.00 0.00 0.04093 24800.00 11800.00 0.00 0.04111 24600.00 12000.00 0.00 0.04134 24800.00 12000.00 0.00 0.04154 25200.00 12000.00 0.00 0.04157 24600.00 12200.00 0.00 0.04197 24800.00 12200.00 0.00 0.04222 25000.00 12200.00 0.00 0.04227 25200.00 12200.00 0.00 0.04233 24600.00 12400.00 0.00 0.04264 24800.00 12400.00 0.00 0.04292 25000.00 12400.00 0.00 0.04299 25200.00 12400.00 0.00 0.04301 24600.00 12600.00 0.00 0.04329 24800.00 12600.00 0.00 0.04360 25000.00 12600.00 0.00 0.04364 24600.00 12800.00 0.00 0.04393 24800.00 12800.00 0.00 0.04424 24600.00 13000.00 0.00 0.04457 24800.00 13000.00 0.00 0.04487 24600.00 13200.00 0.00 0.04541 24800.00 13200.00 0.00 0.04567 24600.00 13400.00 0.00 0.04614 24800.00 13400.00 0.00 0.04636 24600.00 13600.00 0.00 0.04685 24800.00 13600.00 0.00 0.04702 24600.00 13800.00 0.00 0.04750 24800.00 13800.00 0.00 0.04764 24600.00 14000.00 0.00 0.04808 24800.00 14000.00 0.00 0.04821 24600.00 14200.00 0.00 0.04884 24800.00 14200.00 0.00 0.04894 24600.00 14400.00 0.00 0.04951 24800.00 14400.00 0.00 0.04957 24600.00 14600.00 0.00 0.05018 24800.00 14600.00 0.00 0.05020 24600.00 14800.00 0.00 0.05083 24800.00 14800.00 0.00 0.05083 24400.00 15000.00 0.00 0.05092 24600.00 15000.00 0.00 0.05159 24800.00 15000.00 0.00 0.05160 24400.00 15200.00 0.00 0.05179 24600.00 15200.00 0.00 0.05244 24800.00 15200.00 0.00 0.05247 24400.00 15400.00 0.00 0.05259 24600.00 15400.00 0.00 0.05328 24800.00 15400.00 0.00 0.05337 24400.00 15600.00 0.00 0.05355 24600.00 15600.00 0.00 0.05431 24800.00 15600.00 0.00 0.05443 24400.00 15800.00 0.00 0.05456 24600.00 15800.00 0.00 0.05533 24800.00 15800.00 0.00 0.05543 24400.00 16000.00 0.00 0.05558 24600.00 16000.00 0.00 0.05633 24800.00 16000.00 0.00 0.05638 24400.00 16200.00 0.00 0.05660 24600.00 16200.00 0.00 0.05733 24800.00 16200.00 0.00 0.05735 24400.00 16400.00 0.00 0.05774 24600.00 16400.00 0.00 0.05844 24800.00 16400.00 0.00 0.05845 24400.00 16600.00 0.00 0.05891 24600.00 16600.00 0.00 0.05958 24400.00 16800.00 0.00 0.06014 24600.00 16800.00 0.00 0.06080 24400.00 17000.00 0.00 0.06149 24600.00 17000.00 0.00 0.06216 24800.00 17000.00 0.00 0.06216 24400.00 17200.00 0.00 0.06277 24600.00 17200.00 0.00 0.06341 24400.00 17400.00 0.00 0.06399 24600.00 17400.00 0.00 0.06464 24400.00 17600.00 0.00 0.06526 24600.00 17600.00 0.00 0.06594 24400.00 17800.00 0.00 0.06669 24600.00 17800.00 0.00 0.06734 24400.00 18000.00 0.00 0.06809 24600.00 18000.00 0.00 0.06865 24400.00 18200.00 0.00 0.06939 24600.00 18200.00 0.00 0.06989 24400.00 18400.00 0.00 0.07068 24600.00 18400.00 0.00 0.07119 24400.00 18600.00 0.00 0.07218 24600.00 18600.00 0.00 0.07270 24400.00 18800.00 0.00 0.07383 24600.00 18800.00 0.00 0.07431 24400.00 19000.00 0.00 0.07541 24600.00 19000.00 0.00 0.07587 24400.00 19200.00 0.00 0.07721 24600.00 19200.00 0.00 0.07762 24400.00 19400.00 0.00 0.07903 24600.00 19400.00 0.00 0.07937 24400.00 19600.00 0.00 0.08080 24600.00 19600.00 0.00 0.08106 24200.00 19800.00 0.00 0.08112 24400.00 19800.00 0.00 0.08251 24600.00 19800.00 0.00 0.08274 24200.00 20000.00 0.00 0.08283 24400.00 20000.00 0.00 0.08419 24600.00 20000.00 0.00 0.08443 24200.00 20200.00 0.00 0.08453 24400.00 20200.00 0.00 0.08590 24600.00 20200.00 0.00 0.08616 24200.00 20400.00 0.00 0.08626 24400.00 20400.00 0.00 0.08763 24600.00 20400.00 0.00 0.08787 24200.00 20600.00 0.00 0.08816 24400.00 20600.00 0.00 0.08949 24600.00 20600.00 0.00 0.08968 24200.00 20800.00 0.00 0.09006 24400.00 20800.00 0.00 0.09136 24600.00 20800.00 0.00 0.09149 24200.00 21000.00 0.00 0.09215 24400.00 21000.00 0.00 0.09339 24600.00 21000.00 0.00 0.09347 24200.00 21200.00 0.00 0.09422 24400.00 21200.00 0.00 0.09542 24600.00 21200.00 0.00 0.09545 24200.00 21400.00 0.00 0.09638 24400.00 21400.00 0.00 0.09758 24600.00 21400.00 0.00 0.09761 24200.00 21600.00 0.00 0.09878 24400.00 21600.00 0.00 0.10000 24200.00 21800.00 0.00 0.10123 24400.00 21800.00 0.00 0.10243 24200.00 22000.00 0.00 0.10384 24400.00 22000.00 0.00 0.10496 24200.00 22200.00 0.00 0.10652 24400.00 22200.00 0.00 0.10754 24200.00 22400.00 0.00 0.10941 24400.00 22400.00 0.00 0.11032 24200.00 22600.00 0.00 0.11223 24400.00 22600.00 0.00 0.11301 24200.00 22800.00 0.00 0.11507 24400.00 22800.00 0.00 0.11571 24000.00 23000.00 0.00 0.11584 24200.00 23000.00 0.00 0.11786 24400.00 23000.00 0.00 0.11835 24000.00 23200.00 0.00 0.11874 24200.00 23200.00 0.00 0.12063 24400.00 23200.00 0.00 0.12098 24000.00 23400.00 0.00 0.12184 24200.00 23400.00 0.00 0.12356 24400.00 23400.00 0.00 0.12367 24000.00 23600.00 0.00 0.12466 24200.00 23600.00 0.00 0.12614 24000.00 23800.00 0.00 0.12737 24200.00 23800.00 0.00 0.12849 24000.00 24000.00 0.00 0.12930 24200.00 24000.00 0.00 0.13010 24000.00 24200.00 0.00 0.13011 24200.00 24200.00 0.00 0.13052 24000.00 24200.00 15.00 0.13054 24000.00 24200.00 20.00 0.13066 24000.00 24200.00 25.00 0.13078 24000.00 24200.00 30.00 0.13087 24000.00 24200.00 35.00 0.13094 24000.00 24200.00 40.00 0.13098 24000.00 24200.00 45.00 0.13100 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23981.21 24289.86 45.00 0.13122 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/036.ctf NX, NY, NZ: 512 512 1 MODE : real