CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/035.mrc Output diagnostic file name Micrographs_0601/035.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/035.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2136.000 8518.000 Mean, RMS : 4527.666 224.4379 TITLE 1: emi2mrc: 035.emi Sun Jan 05 13:55:15 2014 TITLE 2: kV 300, Mag 78000x, Def 25100, Spot 4, Int 55.10%, Obj 88.04%, Dif 73.67% TITLE 3: X 140.885, Y 309.929, Z -102.061, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01532 13600.00 10000.00 0.00 -0.01528 13800.00 10000.00 0.00 -0.01525 14200.00 10000.00 0.00 -0.01515 14400.00 10000.00 0.00 -0.01500 14600.00 10000.00 0.00 -0.01492 14800.00 10000.00 0.00 -0.01488 15000.00 10000.00 0.00 -0.01476 15200.00 10000.00 0.00 -0.01457 15400.00 10000.00 0.00 -0.01432 15600.00 10000.00 0.00 -0.01391 15800.00 10000.00 0.00 -0.01337 16000.00 10000.00 0.00 -0.01286 16200.00 10000.00 0.00 -0.01238 16400.00 10000.00 0.00 -0.01177 16600.00 10000.00 0.00 -0.01112 16800.00 10000.00 0.00 -0.01055 17000.00 10000.00 0.00 -0.00988 17200.00 10000.00 0.00 -0.00916 17400.00 10000.00 0.00 -0.00852 17600.00 10000.00 0.00 -0.00787 17800.00 10000.00 0.00 -0.00718 18000.00 10000.00 0.00 -0.00645 18200.00 10000.00 0.00 -0.00572 18400.00 10000.00 0.00 -0.00504 18600.00 10000.00 0.00 -0.00424 18800.00 10000.00 0.00 -0.00337 19000.00 10000.00 0.00 -0.00261 19200.00 10000.00 0.00 -0.00184 19400.00 10000.00 0.00 -0.00111 19600.00 10000.00 0.00 -0.00037 19800.00 10000.00 0.00 0.00045 20000.00 10000.00 0.00 0.00131 20200.00 10000.00 0.00 0.00228 20400.00 10000.00 0.00 0.00337 20600.00 10000.00 0.00 0.00454 20800.00 10000.00 0.00 0.00552 21000.00 10000.00 0.00 0.00634 21200.00 10000.00 0.00 0.00726 21400.00 10000.00 0.00 0.00822 21600.00 10000.00 0.00 0.00936 21800.00 10000.00 0.00 0.01056 22000.00 10000.00 0.00 0.01173 22200.00 10000.00 0.00 0.01287 22400.00 10000.00 0.00 0.01398 22600.00 10000.00 0.00 0.01520 22800.00 10000.00 0.00 0.01656 23000.00 10000.00 0.00 0.01803 23200.00 10000.00 0.00 0.01960 23400.00 10000.00 0.00 0.02122 23600.00 10000.00 0.00 0.02284 23800.00 10000.00 0.00 0.02457 24000.00 10000.00 0.00 0.02643 24200.00 10000.00 0.00 0.02823 24400.00 10000.00 0.00 0.02973 24600.00 10000.00 0.00 0.03085 24800.00 10000.00 0.00 0.03161 25000.00 10000.00 0.00 0.03211 25200.00 10000.00 0.00 0.03246 25400.00 10000.00 0.00 0.03252 25000.00 10200.00 0.00 0.03270 25200.00 10200.00 0.00 0.03301 25000.00 10400.00 0.00 0.03315 25200.00 10400.00 0.00 0.03338 25000.00 10600.00 0.00 0.03346 25200.00 10600.00 0.00 0.03362 25000.00 10800.00 0.00 0.03378 25200.00 10800.00 0.00 0.03391 25000.00 11000.00 0.00 0.03401 25200.00 11000.00 0.00 0.03412 25000.00 11200.00 0.00 0.03428 25200.00 11200.00 0.00 0.03440 25000.00 11400.00 0.00 0.03467 25200.00 11400.00 0.00 0.03478 25000.00 11600.00 0.00 0.03507 25200.00 11600.00 0.00 0.03516 24800.00 11800.00 0.00 0.03521 25000.00 11800.00 0.00 0.03556 25200.00 11800.00 0.00 0.03564 24800.00 12000.00 0.00 0.03578 25000.00 12000.00 0.00 0.03612 25200.00 12000.00 0.00 0.03620 24800.00 12200.00 0.00 0.03645 25000.00 12200.00 0.00 0.03676 25200.00 12200.00 0.00 0.03684 24800.00 12400.00 0.00 0.03705 25000.00 12400.00 0.00 0.03736 25200.00 12400.00 0.00 0.03745 24800.00 12600.00 0.00 0.03762 25000.00 12600.00 0.00 0.03794 25200.00 12600.00 0.00 0.03803 24800.00 12800.00 0.00 0.03822 25000.00 12800.00 0.00 0.03853 25200.00 12800.00 0.00 0.03860 24800.00 13000.00 0.00 0.03877 25000.00 13000.00 0.00 0.03910 25200.00 13000.00 0.00 0.03916 24800.00 13200.00 0.00 0.03944 25000.00 13200.00 0.00 0.03981 25200.00 13200.00 0.00 0.03989 24800.00 13400.00 0.00 0.04023 25000.00 13400.00 0.00 0.04061 25200.00 13400.00 0.00 0.04065 24800.00 13600.00 0.00 0.04108 25000.00 13600.00 0.00 0.04142 24800.00 13800.00 0.00 0.04175 25000.00 13800.00 0.00 0.04202 24800.00 14000.00 0.00 0.04233 25000.00 14000.00 0.00 0.04255 24800.00 14200.00 0.00 0.04312 25000.00 14200.00 0.00 0.04329 24800.00 14400.00 0.00 0.04389 25000.00 14400.00 0.00 0.04399 24800.00 14600.00 0.00 0.04454 25000.00 14600.00 0.00 0.04458 24600.00 14800.00 0.00 0.04461 24800.00 14800.00 0.00 0.04518 25000.00 14800.00 0.00 0.04520 24600.00 15000.00 0.00 0.04537 24800.00 15000.00 0.00 0.04592 25000.00 15000.00 0.00 0.04594 24600.00 15200.00 0.00 0.04608 24800.00 15200.00 0.00 0.04663 25000.00 15200.00 0.00 0.04669 24600.00 15400.00 0.00 0.04688 24800.00 15400.00 0.00 0.04744 25000.00 15400.00 0.00 0.04751 24600.00 15600.00 0.00 0.04771 24800.00 15600.00 0.00 0.04826 25000.00 15600.00 0.00 0.04833 24600.00 15800.00 0.00 0.04848 24800.00 15800.00 0.00 0.04905 25000.00 15800.00 0.00 0.04915 24600.00 16000.00 0.00 0.04955 24800.00 16000.00 0.00 0.05013 25000.00 16000.00 0.00 0.05027 24600.00 16200.00 0.00 0.05069 24800.00 16200.00 0.00 0.05130 25000.00 16200.00 0.00 0.05148 25200.00 16200.00 0.00 0.05150 24600.00 16400.00 0.00 0.05186 24800.00 16400.00 0.00 0.05252 25000.00 16400.00 0.00 0.05273 24600.00 16600.00 0.00 0.05300 24800.00 16600.00 0.00 0.05369 25000.00 16600.00 0.00 0.05386 24600.00 16800.00 0.00 0.05418 24800.00 16800.00 0.00 0.05489 25000.00 16800.00 0.00 0.05504 24600.00 17000.00 0.00 0.05557 24800.00 17000.00 0.00 0.05627 25000.00 17000.00 0.00 0.05640 24600.00 17200.00 0.00 0.05683 24800.00 17200.00 0.00 0.05753 25000.00 17200.00 0.00 0.05766 24600.00 17400.00 0.00 0.05806 24800.00 17400.00 0.00 0.05881 25000.00 17400.00 0.00 0.05898 24600.00 17600.00 0.00 0.05943 24800.00 17600.00 0.00 0.06023 25000.00 17600.00 0.00 0.06039 24600.00 17800.00 0.00 0.06089 24800.00 17800.00 0.00 0.06168 25000.00 17800.00 0.00 0.06176 24600.00 18000.00 0.00 0.06218 24800.00 18000.00 0.00 0.06292 25000.00 18000.00 0.00 0.06293 24600.00 18200.00 0.00 0.06342 24800.00 18200.00 0.00 0.06414 24600.00 18400.00 0.00 0.06472 24800.00 18400.00 0.00 0.06542 24600.00 18600.00 0.00 0.06603 24800.00 18600.00 0.00 0.06668 24600.00 18800.00 0.00 0.06746 24800.00 18800.00 0.00 0.06805 24600.00 19000.00 0.00 0.06890 24800.00 19000.00 0.00 0.06941 24600.00 19200.00 0.00 0.07032 24800.00 19200.00 0.00 0.07080 24600.00 19400.00 0.00 0.07167 24800.00 19400.00 0.00 0.07218 24600.00 19600.00 0.00 0.07311 24800.00 19600.00 0.00 0.07365 24600.00 19800.00 0.00 0.07452 24800.00 19800.00 0.00 0.07508 24600.00 20000.00 0.00 0.07599 24800.00 20000.00 0.00 0.07655 24600.00 20200.00 0.00 0.07767 24800.00 20200.00 0.00 0.07815 24600.00 20400.00 0.00 0.07927 24800.00 20400.00 0.00 0.07962 24600.00 20600.00 0.00 0.08095 24800.00 20600.00 0.00 0.08120 24400.00 20800.00 0.00 0.08131 24600.00 20800.00 0.00 0.08265 24800.00 20800.00 0.00 0.08286 24400.00 21000.00 0.00 0.08322 24600.00 21000.00 0.00 0.08452 24800.00 21000.00 0.00 0.08470 24400.00 21200.00 0.00 0.08518 24600.00 21200.00 0.00 0.08642 24800.00 21200.00 0.00 0.08659 24400.00 21400.00 0.00 0.08714 24600.00 21400.00 0.00 0.08835 24800.00 21400.00 0.00 0.08850 24400.00 21600.00 0.00 0.08918 24600.00 21600.00 0.00 0.09036 24800.00 21600.00 0.00 0.09045 24400.00 21800.00 0.00 0.09127 24600.00 21800.00 0.00 0.09240 24800.00 21800.00 0.00 0.09241 24400.00 22000.00 0.00 0.09355 24600.00 22000.00 0.00 0.09461 24400.00 22200.00 0.00 0.09581 24600.00 22200.00 0.00 0.09682 24400.00 22400.00 0.00 0.09826 24600.00 22400.00 0.00 0.09925 24400.00 22600.00 0.00 0.10079 24600.00 22600.00 0.00 0.10175 24400.00 22800.00 0.00 0.10341 24600.00 22800.00 0.00 0.10424 24400.00 23000.00 0.00 0.10606 24600.00 23000.00 0.00 0.10673 24400.00 23200.00 0.00 0.10886 24600.00 23200.00 0.00 0.10937 24200.00 23400.00 0.00 0.10966 24400.00 23400.00 0.00 0.11187 24600.00 23400.00 0.00 0.11219 24200.00 23600.00 0.00 0.11275 24400.00 23600.00 0.00 0.11483 24600.00 23600.00 0.00 0.11487 24200.00 23800.00 0.00 0.11592 24400.00 23800.00 0.00 0.11776 24200.00 24000.00 0.00 0.11872 24400.00 24000.00 0.00 0.12030 24200.00 24200.00 0.00 0.12142 24400.00 24200.00 0.00 0.12248 24200.00 24400.00 0.00 0.12260 24400.00 24400.00 0.00 0.12279 24200.00 24400.00 10.00 0.12291 24200.00 24400.00 15.00 0.12306 24200.00 24400.00 20.00 0.12319 24200.00 24400.00 25.00 0.12330 24200.00 24400.00 30.00 0.12338 24200.00 24400.00 35.00 0.12344 24200.00 24400.00 40.00 0.12347 24200.00 24400.00 45.00 0.12347 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24242.78 24434.74 42.69 0.12361 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/035.ctf NX, NY, NZ: 512 512 1 MODE : real