CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/034.mrc Output diagnostic file name Micrographs_0601/034.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/034.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2368.000 7518.000 Mean, RMS : 4524.642 225.5517 TITLE 1: emi2mrc: 034.emi Sun Jan 05 13:54:26 2014 TITLE 2: kV 300, Mag 78000x, Def 25100, Spot 4, Int 55.10%, Obj 88.04%, Dif 73.67% TITLE 3: X 138.367, Y 310.315, Z -102.061, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01008 14400.00 10000.00 0.00 -0.01000 14600.00 10000.00 0.00 -0.00982 14800.00 10000.00 0.00 -0.00951 15000.00 10000.00 0.00 -0.00911 15200.00 10000.00 0.00 -0.00878 15400.00 10000.00 0.00 -0.00831 15600.00 10000.00 0.00 -0.00787 15800.00 10000.00 0.00 -0.00750 16000.00 10000.00 0.00 -0.00713 16200.00 10000.00 0.00 -0.00685 16400.00 10000.00 0.00 -0.00662 16600.00 10000.00 0.00 -0.00629 16800.00 10000.00 0.00 -0.00581 17000.00 10000.00 0.00 -0.00533 17200.00 10000.00 0.00 -0.00490 17400.00 10000.00 0.00 -0.00446 17600.00 10000.00 0.00 -0.00383 17800.00 10000.00 0.00 -0.00308 18000.00 10000.00 0.00 -0.00250 18200.00 10000.00 0.00 -0.00196 18400.00 10000.00 0.00 -0.00134 18600.00 10000.00 0.00 -0.00068 18800.00 10000.00 0.00 0.00004 19000.00 10000.00 0.00 0.00077 19200.00 10000.00 0.00 0.00153 19400.00 10000.00 0.00 0.00224 19600.00 10000.00 0.00 0.00288 19800.00 10000.00 0.00 0.00363 20000.00 10000.00 0.00 0.00457 20200.00 10000.00 0.00 0.00559 20400.00 10000.00 0.00 0.00652 20600.00 10000.00 0.00 0.00735 20800.00 10000.00 0.00 0.00822 21000.00 10000.00 0.00 0.00915 21200.00 10000.00 0.00 0.01009 21400.00 10000.00 0.00 0.01104 21600.00 10000.00 0.00 0.01186 21800.00 10000.00 0.00 0.01271 22000.00 10000.00 0.00 0.01393 22200.00 10000.00 0.00 0.01518 22400.00 10000.00 0.00 0.01633 22600.00 10000.00 0.00 0.01753 22800.00 10000.00 0.00 0.01870 23000.00 10000.00 0.00 0.01988 23200.00 10000.00 0.00 0.02127 23400.00 10000.00 0.00 0.02280 23600.00 10000.00 0.00 0.02431 23800.00 10000.00 0.00 0.02581 24000.00 10000.00 0.00 0.02731 24200.00 10000.00 0.00 0.02898 24400.00 10000.00 0.00 0.03082 24600.00 10000.00 0.00 0.03273 24800.00 10000.00 0.00 0.03475 25000.00 10000.00 0.00 0.03638 25200.00 10000.00 0.00 0.03743 25400.00 10000.00 0.00 0.03813 25600.00 10000.00 0.00 0.03849 25800.00 10000.00 0.00 0.03851 25600.00 10200.00 0.00 0.03872 25600.00 10400.00 0.00 0.03898 25400.00 10600.00 0.00 0.03908 25600.00 10600.00 0.00 0.03938 25400.00 10800.00 0.00 0.03956 25600.00 10800.00 0.00 0.03982 25400.00 11000.00 0.00 0.04006 25600.00 11000.00 0.00 0.04026 25400.00 11200.00 0.00 0.04043 25600.00 11200.00 0.00 0.04060 25400.00 11400.00 0.00 0.04068 25600.00 11400.00 0.00 0.04087 25800.00 11400.00 0.00 0.04088 25400.00 11600.00 0.00 0.04092 25600.00 11600.00 0.00 0.04114 25400.00 11800.00 0.00 0.04129 25600.00 11800.00 0.00 0.04149 25400.00 12000.00 0.00 0.04163 25600.00 12000.00 0.00 0.04178 25400.00 12200.00 0.00 0.04190 25600.00 12200.00 0.00 0.04204 25400.00 12400.00 0.00 0.04210 25600.00 12400.00 0.00 0.04222 25400.00 12600.00 0.00 0.04231 25600.00 12600.00 0.00 0.04242 25400.00 12800.00 0.00 0.04271 25600.00 12800.00 0.00 0.04283 25400.00 13000.00 0.00 0.04311 25600.00 13000.00 0.00 0.04324 25400.00 13200.00 0.00 0.04368 25600.00 13200.00 0.00 0.04382 25400.00 13400.00 0.00 0.04422 25600.00 13400.00 0.00 0.04437 25400.00 13600.00 0.00 0.04470 25600.00 13600.00 0.00 0.04486 25400.00 13800.00 0.00 0.04528 25600.00 13800.00 0.00 0.04544 25400.00 14000.00 0.00 0.04585 25600.00 14000.00 0.00 0.04602 25200.00 14200.00 0.00 0.04606 25400.00 14200.00 0.00 0.04659 25600.00 14200.00 0.00 0.04679 25800.00 14200.00 0.00 0.04679 25200.00 14400.00 0.00 0.04681 25400.00 14400.00 0.00 0.04734 25600.00 14400.00 0.00 0.04754 25400.00 14600.00 0.00 0.04805 25600.00 14600.00 0.00 0.04823 25400.00 14800.00 0.00 0.04869 25600.00 14800.00 0.00 0.04885 25400.00 15000.00 0.00 0.04923 25600.00 15000.00 0.00 0.04946 25800.00 15000.00 0.00 0.04950 25400.00 15200.00 0.00 0.04990 25600.00 15200.00 0.00 0.05021 25800.00 15200.00 0.00 0.05026 25400.00 15400.00 0.00 0.05070 25600.00 15400.00 0.00 0.05100 25400.00 15600.00 0.00 0.05151 25600.00 15600.00 0.00 0.05176 25400.00 15800.00 0.00 0.05227 25600.00 15800.00 0.00 0.05246 25200.00 16000.00 0.00 0.05250 25400.00 16000.00 0.00 0.05311 25600.00 16000.00 0.00 0.05324 25200.00 16200.00 0.00 0.05343 25400.00 16200.00 0.00 0.05399 25600.00 16200.00 0.00 0.05406 25200.00 16400.00 0.00 0.05431 25400.00 16400.00 0.00 0.05483 25600.00 16400.00 0.00 0.05488 25200.00 16600.00 0.00 0.05515 25400.00 16600.00 0.00 0.05567 25600.00 16600.00 0.00 0.05571 25200.00 16800.00 0.00 0.05601 25400.00 16800.00 0.00 0.05655 25600.00 16800.00 0.00 0.05657 25200.00 17000.00 0.00 0.05702 25400.00 17000.00 0.00 0.05753 25200.00 17200.00 0.00 0.05815 25400.00 17200.00 0.00 0.05860 25200.00 17400.00 0.00 0.05921 25400.00 17400.00 0.00 0.05958 25200.00 17600.00 0.00 0.06008 25400.00 17600.00 0.00 0.06038 25200.00 17800.00 0.00 0.06109 25400.00 17800.00 0.00 0.06142 25200.00 18000.00 0.00 0.06221 25400.00 18000.00 0.00 0.06256 25200.00 18200.00 0.00 0.06333 25400.00 18200.00 0.00 0.06368 25200.00 18400.00 0.00 0.06442 25400.00 18400.00 0.00 0.06478 25200.00 18600.00 0.00 0.06553 25400.00 18600.00 0.00 0.06589 25200.00 18800.00 0.00 0.06676 25400.00 18800.00 0.00 0.06710 25200.00 19000.00 0.00 0.06793 25400.00 19000.00 0.00 0.06823 25200.00 19200.00 0.00 0.06917 25400.00 19200.00 0.00 0.06948 25200.00 19400.00 0.00 0.07051 25400.00 19400.00 0.00 0.07081 25000.00 19600.00 0.00 0.07083 25200.00 19600.00 0.00 0.07197 25400.00 19600.00 0.00 0.07222 25000.00 19800.00 0.00 0.07231 25200.00 19800.00 0.00 0.07342 25400.00 19800.00 0.00 0.07363 25000.00 20000.00 0.00 0.07380 25200.00 20000.00 0.00 0.07486 25400.00 20000.00 0.00 0.07503 25000.00 20200.00 0.00 0.07539 25200.00 20200.00 0.00 0.07641 25400.00 20200.00 0.00 0.07658 25000.00 20400.00 0.00 0.07703 25200.00 20400.00 0.00 0.07806 25400.00 20400.00 0.00 0.07828 25000.00 20600.00 0.00 0.07890 25200.00 20600.00 0.00 0.07994 25400.00 20600.00 0.00 0.08017 25000.00 20800.00 0.00 0.08074 25200.00 20800.00 0.00 0.08178 25400.00 20800.00 0.00 0.08201 25000.00 21000.00 0.00 0.08252 25200.00 21000.00 0.00 0.08358 25400.00 21000.00 0.00 0.08379 25000.00 21200.00 0.00 0.08429 25200.00 21200.00 0.00 0.08537 25400.00 21200.00 0.00 0.08554 25000.00 21400.00 0.00 0.08615 25200.00 21400.00 0.00 0.08724 25400.00 21400.00 0.00 0.08734 25000.00 21600.00 0.00 0.08812 25200.00 21600.00 0.00 0.08920 25400.00 21600.00 0.00 0.08922 25000.00 21800.00 0.00 0.09017 25200.00 21800.00 0.00 0.09120 25000.00 22000.00 0.00 0.09224 25200.00 22000.00 0.00 0.09319 25000.00 22200.00 0.00 0.09433 25200.00 22200.00 0.00 0.09522 25000.00 22400.00 0.00 0.09667 25200.00 22400.00 0.00 0.09745 25000.00 22600.00 0.00 0.09892 25200.00 22600.00 0.00 0.09955 25000.00 22800.00 0.00 0.10118 25200.00 22800.00 0.00 0.10170 25000.00 23000.00 0.00 0.10355 25200.00 23000.00 0.00 0.10401 24800.00 23200.00 0.00 0.10401 25000.00 23200.00 0.00 0.10591 25200.00 23200.00 0.00 0.10627 24800.00 23400.00 0.00 0.10645 25000.00 23400.00 0.00 0.10830 25200.00 23400.00 0.00 0.10855 24800.00 23600.00 0.00 0.10898 25000.00 23600.00 0.00 0.11079 25200.00 23600.00 0.00 0.11090 24800.00 23800.00 0.00 0.11178 25000.00 23800.00 0.00 0.11349 24800.00 24000.00 0.00 0.11454 25000.00 24000.00 0.00 0.11611 24800.00 24200.00 0.00 0.11754 25000.00 24200.00 0.00 0.11891 24800.00 24400.00 0.00 0.12039 25000.00 24400.00 0.00 0.12151 24800.00 24600.00 0.00 0.12327 25000.00 24600.00 0.00 0.12395 24800.00 24800.00 0.00 0.12555 24800.00 25000.00 5.00 0.12559 24800.00 25000.00 10.00 0.12575 24800.00 25000.00 15.00 0.12591 24800.00 25000.00 20.00 0.12606 24800.00 25000.00 25.00 0.12619 24800.00 25000.00 30.00 0.12630 24800.00 25000.00 35.00 0.12638 24800.00 25000.00 40.00 0.12643 24800.00 25000.00 45.00 0.12645 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24753.79 24992.10 45.00 0.12654 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/034.ctf NX, NY, NZ: 512 512 1 MODE : real