CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/033.mrc Output diagnostic file name Micrographs_0601/033.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/033.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2430.000 8954.000 Mean, RMS : 4528.452 225.5842 TITLE 1: emi2mrc: 033.emi Sun Jan 05 13:53:27 2014 TITLE 2: kV 300, Mag 78000x, Def 25100, Spot 4, Int 55.10%, Obj 88.04%, Dif 73.67% TITLE 3: X 135.906, Y 310.579, Z -102.061, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01253 15600.00 10000.00 0.00 -0.01245 15800.00 10000.00 0.00 -0.01219 16000.00 10000.00 0.00 -0.01165 16200.00 10000.00 0.00 -0.01108 16400.00 10000.00 0.00 -0.01066 16600.00 10000.00 0.00 -0.01028 16800.00 10000.00 0.00 -0.01005 17000.00 10000.00 0.00 -0.00978 17200.00 10000.00 0.00 -0.00933 17400.00 10000.00 0.00 -0.00880 17600.00 10000.00 0.00 -0.00815 17800.00 10000.00 0.00 -0.00767 18000.00 10000.00 0.00 -0.00733 18200.00 10000.00 0.00 -0.00683 18400.00 10000.00 0.00 -0.00642 18600.00 10000.00 0.00 -0.00597 18800.00 10000.00 0.00 -0.00538 19000.00 10000.00 0.00 -0.00474 19200.00 10000.00 0.00 -0.00401 19400.00 10000.00 0.00 -0.00337 19600.00 10000.00 0.00 -0.00271 19800.00 10000.00 0.00 -0.00183 20000.00 10000.00 0.00 -0.00087 20200.00 10000.00 0.00 -0.00002 20400.00 10000.00 0.00 0.00071 20600.00 10000.00 0.00 0.00139 20800.00 10000.00 0.00 0.00208 21000.00 10000.00 0.00 0.00285 21200.00 10000.00 0.00 0.00361 21400.00 10000.00 0.00 0.00438 21600.00 10000.00 0.00 0.00527 21800.00 10000.00 0.00 0.00609 22000.00 10000.00 0.00 0.00689 22200.00 10000.00 0.00 0.00771 22400.00 10000.00 0.00 0.00861 22600.00 10000.00 0.00 0.00959 22800.00 10000.00 0.00 0.01058 23000.00 10000.00 0.00 0.01169 23200.00 10000.00 0.00 0.01289 23400.00 10000.00 0.00 0.01416 23600.00 10000.00 0.00 0.01553 23800.00 10000.00 0.00 0.01686 24000.00 10000.00 0.00 0.01826 24200.00 10000.00 0.00 0.01984 24400.00 10000.00 0.00 0.02140 24600.00 10000.00 0.00 0.02290 24800.00 10000.00 0.00 0.02450 25000.00 10000.00 0.00 0.02620 25200.00 10000.00 0.00 0.02807 25400.00 10000.00 0.00 0.03000 25600.00 10000.00 0.00 0.03161 25800.00 10000.00 0.00 0.03271 26000.00 10000.00 0.00 0.03325 26200.00 10000.00 0.00 0.03342 26000.00 10200.00 0.00 0.03352 26200.00 10200.00 0.00 0.03367 26000.00 10400.00 0.00 0.03370 26200.00 10400.00 0.00 0.03384 26000.00 10600.00 0.00 0.03387 26200.00 10600.00 0.00 0.03405 26000.00 10800.00 0.00 0.03413 26200.00 10800.00 0.00 0.03435 26000.00 11000.00 0.00 0.03450 26200.00 11000.00 0.00 0.03472 26000.00 11200.00 0.00 0.03477 26200.00 11200.00 0.00 0.03493 26000.00 11400.00 0.00 0.03498 26200.00 11400.00 0.00 0.03509 26000.00 11600.00 0.00 0.03521 26200.00 11600.00 0.00 0.03529 26000.00 11800.00 0.00 0.03550 26200.00 11800.00 0.00 0.03558 26000.00 12000.00 0.00 0.03580 26200.00 12000.00 0.00 0.03587 26000.00 12200.00 0.00 0.03610 26200.00 12200.00 0.00 0.03615 26000.00 12400.00 0.00 0.03638 26200.00 12400.00 0.00 0.03640 26000.00 12600.00 0.00 0.03666 26200.00 12600.00 0.00 0.03668 25800.00 12800.00 0.00 0.03669 26000.00 12800.00 0.00 0.03705 26200.00 12800.00 0.00 0.03707 26000.00 13000.00 0.00 0.03735 26200.00 13000.00 0.00 0.03737 26000.00 13200.00 0.00 0.03773 26200.00 13200.00 0.00 0.03775 25800.00 13400.00 0.00 0.03775 26000.00 13400.00 0.00 0.03811 26200.00 13400.00 0.00 0.03813 26000.00 13600.00 0.00 0.03847 26200.00 13600.00 0.00 0.03853 25800.00 13800.00 0.00 0.03863 26000.00 13800.00 0.00 0.03904 26200.00 13800.00 0.00 0.03913 25800.00 14000.00 0.00 0.03920 26000.00 14000.00 0.00 0.03959 26200.00 14000.00 0.00 0.03967 26000.00 14200.00 0.00 0.04006 26200.00 14200.00 0.00 0.04016 25800.00 14400.00 0.00 0.04025 26000.00 14400.00 0.00 0.04065 26200.00 14400.00 0.00 0.04077 25800.00 14600.00 0.00 0.04092 26000.00 14600.00 0.00 0.04134 26200.00 14600.00 0.00 0.04146 25800.00 14800.00 0.00 0.04153 26000.00 14800.00 0.00 0.04197 26200.00 14800.00 0.00 0.04207 25800.00 15000.00 0.00 0.04226 26000.00 15000.00 0.00 0.04267 26200.00 15000.00 0.00 0.04274 25800.00 15200.00 0.00 0.04306 26000.00 15200.00 0.00 0.04343 26200.00 15200.00 0.00 0.04350 25800.00 15400.00 0.00 0.04383 26000.00 15400.00 0.00 0.04419 26200.00 15400.00 0.00 0.04428 25800.00 15600.00 0.00 0.04462 26000.00 15600.00 0.00 0.04497 26200.00 15600.00 0.00 0.04507 25800.00 15800.00 0.00 0.04536 26000.00 15800.00 0.00 0.04572 26200.00 15800.00 0.00 0.04579 25800.00 16000.00 0.00 0.04615 26000.00 16000.00 0.00 0.04651 26200.00 16000.00 0.00 0.04656 25800.00 16200.00 0.00 0.04698 26000.00 16200.00 0.00 0.04730 25800.00 16400.00 0.00 0.04776 26000.00 16400.00 0.00 0.04802 25800.00 16600.00 0.00 0.04843 26000.00 16600.00 0.00 0.04865 25800.00 16800.00 0.00 0.04914 26000.00 16800.00 0.00 0.04938 25800.00 17000.00 0.00 0.04996 26000.00 17000.00 0.00 0.05023 26200.00 17000.00 0.00 0.05024 25800.00 17200.00 0.00 0.05081 26000.00 17200.00 0.00 0.05112 26200.00 17200.00 0.00 0.05115 25800.00 17400.00 0.00 0.05173 26000.00 17400.00 0.00 0.05207 25800.00 17600.00 0.00 0.05256 26000.00 17600.00 0.00 0.05290 25800.00 17800.00 0.00 0.05348 26000.00 17800.00 0.00 0.05381 25800.00 18000.00 0.00 0.05446 26000.00 18000.00 0.00 0.05480 25800.00 18200.00 0.00 0.05554 26000.00 18200.00 0.00 0.05592 25800.00 18400.00 0.00 0.05686 26000.00 18400.00 0.00 0.05728 25800.00 18600.00 0.00 0.05817 26000.00 18600.00 0.00 0.05859 25800.00 18800.00 0.00 0.05954 26000.00 18800.00 0.00 0.05995 25800.00 19000.00 0.00 0.06101 26000.00 19000.00 0.00 0.06139 25800.00 19200.00 0.00 0.06245 26000.00 19200.00 0.00 0.06281 25800.00 19400.00 0.00 0.06390 26000.00 19400.00 0.00 0.06421 25800.00 19600.00 0.00 0.06536 26000.00 19600.00 0.00 0.06557 25800.00 19800.00 0.00 0.06659 26000.00 19800.00 0.00 0.06673 25800.00 20000.00 0.00 0.06773 26000.00 20000.00 0.00 0.06786 25800.00 20200.00 0.00 0.06898 26000.00 20200.00 0.00 0.06911 25600.00 20400.00 0.00 0.06926 25800.00 20400.00 0.00 0.07041 26000.00 20400.00 0.00 0.07051 25600.00 20600.00 0.00 0.07085 25800.00 20600.00 0.00 0.07197 26000.00 20600.00 0.00 0.07200 25600.00 20800.00 0.00 0.07240 25800.00 20800.00 0.00 0.07346 25600.00 21000.00 0.00 0.07397 25800.00 21000.00 0.00 0.07499 25600.00 21200.00 0.00 0.07552 25800.00 21200.00 0.00 0.07655 25600.00 21400.00 0.00 0.07718 25800.00 21400.00 0.00 0.07823 25600.00 21600.00 0.00 0.07885 25800.00 21600.00 0.00 0.07990 25600.00 21800.00 0.00 0.08049 25800.00 21800.00 0.00 0.08149 25600.00 22000.00 0.00 0.08224 25800.00 22000.00 0.00 0.08321 25600.00 22200.00 0.00 0.08404 25800.00 22200.00 0.00 0.08497 25600.00 22400.00 0.00 0.08598 25800.00 22400.00 0.00 0.08684 25600.00 22600.00 0.00 0.08780 25800.00 22600.00 0.00 0.08860 25600.00 22800.00 0.00 0.08959 25800.00 22800.00 0.00 0.09038 25600.00 23000.00 0.00 0.09150 25800.00 23000.00 0.00 0.09225 25600.00 23200.00 0.00 0.09355 25800.00 23200.00 0.00 0.09420 25600.00 23400.00 0.00 0.09575 25800.00 23400.00 0.00 0.09630 25600.00 23600.00 0.00 0.09793 25800.00 23600.00 0.00 0.09842 25600.00 23800.00 0.00 0.10014 25800.00 23800.00 0.00 0.10059 25600.00 24000.00 0.00 0.10232 25800.00 24000.00 0.00 0.10270 25600.00 24200.00 0.00 0.10464 25800.00 24200.00 0.00 0.10492 25400.00 24400.00 0.00 0.10494 25600.00 24400.00 0.00 0.10688 25800.00 24400.00 0.00 0.10701 25400.00 24600.00 0.00 0.10740 25600.00 24600.00 0.00 0.10925 25400.00 24800.00 0.00 0.10997 25600.00 24800.00 0.00 0.11168 25400.00 25000.00 0.00 0.11246 25600.00 25000.00 0.00 0.11401 25400.00 25200.00 0.00 0.11494 25600.00 25200.00 0.00 0.11626 25400.00 25400.00 0.00 0.11690 25600.00 25400.00 0.00 0.11798 25400.00 25600.00 0.00 0.11851 25600.00 25600.00 0.00 0.11906 25400.00 25800.00 20.00 0.11917 25400.00 25800.00 25.00 0.11928 25400.00 25800.00 30.00 0.11933 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 25474.23 25698.47 31.82 0.11966 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/033.ctf NX, NY, NZ: 512 512 1 MODE : real