CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/029.mrc Output diagnostic file name Micrographs_0601/029.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/029.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2534.000 7846.000 Mean, RMS : 4517.456 221.5548 TITLE 1: emi2mrc: 029.emi Sun Jan 05 13:49:47 2014 TITLE 2: kV 300, Mag 78000x, Def 25100, Spot 4, Int 55.10%, Obj 88.05%, Dif 73.67% TITLE 3: X 135.531, Y 313.203, Z -101.718, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01397 10400.00 10000.00 0.00 -0.01394 12200.00 10000.00 0.00 -0.01388 12400.00 10000.00 0.00 -0.01379 12600.00 10000.00 0.00 -0.01376 12800.00 10000.00 0.00 -0.01374 13600.00 10000.00 0.00 -0.01358 13800.00 10000.00 0.00 -0.01336 14000.00 10000.00 0.00 -0.01325 14200.00 10000.00 0.00 -0.01319 14400.00 10000.00 0.00 -0.01301 14600.00 10000.00 0.00 -0.01272 14800.00 10000.00 0.00 -0.01238 15000.00 10000.00 0.00 -0.01198 15200.00 10000.00 0.00 -0.01162 15400.00 10000.00 0.00 -0.01129 15600.00 10000.00 0.00 -0.01077 15800.00 10000.00 0.00 -0.01022 16000.00 10000.00 0.00 -0.00989 16200.00 10000.00 0.00 -0.00934 16400.00 10000.00 0.00 -0.00865 16600.00 10000.00 0.00 -0.00814 16800.00 10000.00 0.00 -0.00747 17000.00 10000.00 0.00 -0.00678 17200.00 10000.00 0.00 -0.00619 17400.00 10000.00 0.00 -0.00558 17600.00 10000.00 0.00 -0.00498 17800.00 10000.00 0.00 -0.00433 18000.00 10000.00 0.00 -0.00354 18200.00 10000.00 0.00 -0.00265 18400.00 10000.00 0.00 -0.00182 18600.00 10000.00 0.00 -0.00093 18800.00 10000.00 0.00 0.00005 19000.00 10000.00 0.00 0.00094 19200.00 10000.00 0.00 0.00182 19400.00 10000.00 0.00 0.00277 19600.00 10000.00 0.00 0.00365 19800.00 10000.00 0.00 0.00450 20000.00 10000.00 0.00 0.00541 20200.00 10000.00 0.00 0.00622 20400.00 10000.00 0.00 0.00702 20600.00 10000.00 0.00 0.00788 20800.00 10000.00 0.00 0.00859 21000.00 10000.00 0.00 0.00934 21200.00 10000.00 0.00 0.01020 21400.00 10000.00 0.00 0.01116 21600.00 10000.00 0.00 0.01215 21800.00 10000.00 0.00 0.01313 22000.00 10000.00 0.00 0.01423 22200.00 10000.00 0.00 0.01531 22400.00 10000.00 0.00 0.01647 22600.00 10000.00 0.00 0.01777 22800.00 10000.00 0.00 0.01907 23000.00 10000.00 0.00 0.02039 23200.00 10000.00 0.00 0.02173 23400.00 10000.00 0.00 0.02313 23600.00 10000.00 0.00 0.02458 23800.00 10000.00 0.00 0.02615 24000.00 10000.00 0.00 0.02770 24200.00 10000.00 0.00 0.02925 24400.00 10000.00 0.00 0.03104 24600.00 10000.00 0.00 0.03295 24800.00 10000.00 0.00 0.03475 25000.00 10000.00 0.00 0.03632 25200.00 10000.00 0.00 0.03755 25400.00 10000.00 0.00 0.03810 25600.00 10000.00 0.00 0.03835 25800.00 10000.00 0.00 0.03856 25600.00 10200.00 0.00 0.03877 25800.00 10200.00 0.00 0.03896 25600.00 10400.00 0.00 0.03916 25800.00 10400.00 0.00 0.03932 25600.00 10600.00 0.00 0.03943 25800.00 10600.00 0.00 0.03959 25600.00 10800.00 0.00 0.03975 25800.00 10800.00 0.00 0.03989 25600.00 11000.00 0.00 0.04007 25800.00 11000.00 0.00 0.04017 25400.00 11200.00 0.00 0.04020 25600.00 11200.00 0.00 0.04047 25800.00 11200.00 0.00 0.04058 26000.00 11200.00 0.00 0.04058 25400.00 11400.00 0.00 0.04073 25600.00 11400.00 0.00 0.04097 25800.00 11400.00 0.00 0.04109 26000.00 11400.00 0.00 0.04110 25600.00 11600.00 0.00 0.04131 25800.00 11600.00 0.00 0.04143 26000.00 11600.00 0.00 0.04143 25400.00 11800.00 0.00 0.04153 25600.00 11800.00 0.00 0.04173 25800.00 11800.00 0.00 0.04186 26000.00 11800.00 0.00 0.04187 25400.00 12000.00 0.00 0.04196 25600.00 12000.00 0.00 0.04214 25800.00 12000.00 0.00 0.04229 26000.00 12000.00 0.00 0.04230 25600.00 12200.00 0.00 0.04247 25800.00 12200.00 0.00 0.04267 26000.00 12200.00 0.00 0.04267 25400.00 12400.00 0.00 0.04268 25600.00 12400.00 0.00 0.04296 25800.00 12400.00 0.00 0.04320 25400.00 12600.00 0.00 0.04321 25600.00 12600.00 0.00 0.04352 25800.00 12600.00 0.00 0.04374 25400.00 12800.00 0.00 0.04381 25600.00 12800.00 0.00 0.04414 25800.00 12800.00 0.00 0.04433 25400.00 13000.00 0.00 0.04440 25600.00 13000.00 0.00 0.04473 25800.00 13000.00 0.00 0.04489 25400.00 13200.00 0.00 0.04504 25600.00 13200.00 0.00 0.04537 25800.00 13200.00 0.00 0.04550 25400.00 13400.00 0.00 0.04580 25600.00 13400.00 0.00 0.04610 25800.00 13400.00 0.00 0.04618 25400.00 13600.00 0.00 0.04656 25600.00 13600.00 0.00 0.04681 25800.00 13600.00 0.00 0.04685 25400.00 13800.00 0.00 0.04733 25600.00 13800.00 0.00 0.04755 25800.00 13800.00 0.00 0.04760 25400.00 14000.00 0.00 0.04812 25600.00 14000.00 0.00 0.04834 25800.00 14000.00 0.00 0.04837 25400.00 14200.00 0.00 0.04892 25600.00 14200.00 0.00 0.04912 25400.00 14400.00 0.00 0.04967 25600.00 14400.00 0.00 0.04981 25200.00 14600.00 0.00 0.04992 25400.00 14600.00 0.00 0.05043 25600.00 14600.00 0.00 0.05053 25200.00 14800.00 0.00 0.05069 25400.00 14800.00 0.00 0.05117 25600.00 14800.00 0.00 0.05125 25800.00 14800.00 0.00 0.05126 25200.00 15000.00 0.00 0.05147 25400.00 15000.00 0.00 0.05194 25600.00 15000.00 0.00 0.05205 25800.00 15000.00 0.00 0.05209 25200.00 15200.00 0.00 0.05235 25400.00 15200.00 0.00 0.05283 25600.00 15200.00 0.00 0.05295 25800.00 15200.00 0.00 0.05299 25200.00 15400.00 0.00 0.05329 25400.00 15400.00 0.00 0.05376 25600.00 15400.00 0.00 0.05387 25800.00 15400.00 0.00 0.05388 25200.00 15600.00 0.00 0.05422 25400.00 15600.00 0.00 0.05465 25600.00 15600.00 0.00 0.05474 25200.00 15800.00 0.00 0.05504 25400.00 15800.00 0.00 0.05546 25600.00 15800.00 0.00 0.05555 25200.00 16000.00 0.00 0.05588 25400.00 16000.00 0.00 0.05630 25600.00 16000.00 0.00 0.05640 25200.00 16200.00 0.00 0.05676 25400.00 16200.00 0.00 0.05717 25600.00 16200.00 0.00 0.05724 25200.00 16400.00 0.00 0.05767 25400.00 16400.00 0.00 0.05805 25600.00 16400.00 0.00 0.05808 25200.00 16600.00 0.00 0.05859 25400.00 16600.00 0.00 0.05891 25600.00 16600.00 0.00 0.05892 25200.00 16800.00 0.00 0.05949 25400.00 16800.00 0.00 0.05979 25600.00 16800.00 0.00 0.05981 25200.00 17000.00 0.00 0.06042 25400.00 17000.00 0.00 0.06076 25600.00 17000.00 0.00 0.06084 25200.00 17200.00 0.00 0.06163 25400.00 17200.00 0.00 0.06199 25600.00 17200.00 0.00 0.06207 25200.00 17400.00 0.00 0.06288 25400.00 17400.00 0.00 0.06319 25600.00 17400.00 0.00 0.06320 25200.00 17600.00 0.00 0.06376 25400.00 17600.00 0.00 0.06404 25200.00 17800.00 0.00 0.06473 25400.00 17800.00 0.00 0.06503 25600.00 17800.00 0.00 0.06504 25200.00 18000.00 0.00 0.06574 25400.00 18000.00 0.00 0.06603 25200.00 18200.00 0.00 0.06667 25400.00 18200.00 0.00 0.06694 25200.00 18400.00 0.00 0.06764 25400.00 18400.00 0.00 0.06792 25600.00 18400.00 0.00 0.06792 25200.00 18600.00 0.00 0.06862 25400.00 18600.00 0.00 0.06893 25200.00 18800.00 0.00 0.06965 25400.00 18800.00 0.00 0.06995 25200.00 19000.00 0.00 0.07069 25400.00 19000.00 0.00 0.07098 25200.00 19200.00 0.00 0.07183 25400.00 19200.00 0.00 0.07214 25200.00 19400.00 0.00 0.07300 25400.00 19400.00 0.00 0.07333 25200.00 19600.00 0.00 0.07433 25400.00 19600.00 0.00 0.07467 25200.00 19800.00 0.00 0.07573 25400.00 19800.00 0.00 0.07606 25200.00 20000.00 0.00 0.07713 25400.00 20000.00 0.00 0.07745 25200.00 20200.00 0.00 0.07855 25400.00 20200.00 0.00 0.07885 25200.00 20400.00 0.00 0.07990 25400.00 20400.00 0.00 0.08016 25000.00 20600.00 0.00 0.08017 25200.00 20600.00 0.00 0.08128 25400.00 20600.00 0.00 0.08147 25000.00 20800.00 0.00 0.08153 25200.00 20800.00 0.00 0.08258 25400.00 20800.00 0.00 0.08271 25000.00 21000.00 0.00 0.08287 25200.00 21000.00 0.00 0.08385 25400.00 21000.00 0.00 0.08391 25000.00 21200.00 0.00 0.08406 25200.00 21200.00 0.00 0.08501 25400.00 21200.00 0.00 0.08502 25000.00 21400.00 0.00 0.08527 25200.00 21400.00 0.00 0.08622 25000.00 21600.00 0.00 0.08648 25200.00 21600.00 0.00 0.08740 25000.00 21800.00 0.00 0.08758 25200.00 21800.00 0.00 0.08844 25000.00 22000.00 0.00 0.08874 25200.00 22000.00 0.00 0.08958 25000.00 22200.00 0.00 0.08989 25200.00 22200.00 0.00 0.09074 25000.00 22400.00 0.00 0.09117 25200.00 22400.00 0.00 0.09199 25000.00 22600.00 0.00 0.09244 25200.00 22600.00 0.00 0.09319 25000.00 22800.00 0.00 0.09376 25200.00 22800.00 0.00 0.09444 25000.00 23000.00 0.00 0.09507 25200.00 23000.00 0.00 0.09568 25000.00 23200.00 0.00 0.09639 25200.00 23200.00 0.00 0.09693 25000.00 23400.00 0.00 0.09775 25200.00 23400.00 0.00 0.09820 25000.00 23600.00 0.00 0.09887 25200.00 23600.00 0.00 0.09919 25000.00 23800.00 0.00 0.09993 25200.00 23800.00 0.00 0.10013 25000.00 24000.00 0.00 0.10083 25200.00 24000.00 0.00 0.10089 25000.00 24200.00 0.00 0.10166 25000.00 24400.00 0.00 0.10237 25000.00 24600.00 0.00 0.10323 24800.00 24800.00 0.00 0.10329 25000.00 24800.00 0.00 0.10406 25000.00 25000.00 0.00 0.10443 25000.00 25200.00 0.00 0.10450 25000.00 25200.00 5.00 0.10453 25000.00 25200.00 10.00 0.10456 25000.00 25200.00 15.00 0.10456 24800.00 25200.00 20.00 0.10464 24800.00 25200.00 25.00 0.10470 24800.00 25200.00 30.00 0.10473 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24847.46 25157.79 30.00 0.10476 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/029.ctf NX, NY, NZ: 512 512 1 MODE : real