CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/028.mrc Output diagnostic file name Micrographs_0601/028.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/028.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2356.000 7636.000 Mean, RMS : 4521.666 223.8779 TITLE 1: emi2mrc: 028.emi Sun Jan 05 13:49:09 2014 TITLE 2: kV 300, Mag 78000x, Def 25100, Spot 4, Int 55.10%, Obj 88.05%, Dif 73.67% TITLE 3: X 138.101, Y 312.860, Z -101.718, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00873 10400.00 10000.00 0.00 -0.00872 13200.00 10000.00 0.00 -0.00838 13400.00 10000.00 0.00 -0.00810 13600.00 10000.00 0.00 -0.00779 13800.00 10000.00 0.00 -0.00753 14000.00 10000.00 0.00 -0.00735 14200.00 10000.00 0.00 -0.00707 14400.00 10000.00 0.00 -0.00680 14600.00 10000.00 0.00 -0.00652 14800.00 10000.00 0.00 -0.00618 15000.00 10000.00 0.00 -0.00580 15200.00 10000.00 0.00 -0.00539 15400.00 10000.00 0.00 -0.00505 15600.00 10000.00 0.00 -0.00474 15800.00 10000.00 0.00 -0.00445 16000.00 10000.00 0.00 -0.00413 16200.00 10000.00 0.00 -0.00367 16400.00 10000.00 0.00 -0.00314 16600.00 10000.00 0.00 -0.00261 16800.00 10000.00 0.00 -0.00205 17000.00 10000.00 0.00 -0.00136 17200.00 10000.00 0.00 -0.00050 17400.00 10000.00 0.00 0.00038 17600.00 10000.00 0.00 0.00123 17800.00 10000.00 0.00 0.00209 18000.00 10000.00 0.00 0.00289 18200.00 10000.00 0.00 0.00371 18400.00 10000.00 0.00 0.00466 18600.00 10000.00 0.00 0.00563 18800.00 10000.00 0.00 0.00647 19000.00 10000.00 0.00 0.00732 19200.00 10000.00 0.00 0.00821 19400.00 10000.00 0.00 0.00905 19600.00 10000.00 0.00 0.01001 19800.00 10000.00 0.00 0.01105 20000.00 10000.00 0.00 0.01201 20200.00 10000.00 0.00 0.01303 20400.00 10000.00 0.00 0.01414 20600.00 10000.00 0.00 0.01523 20800.00 10000.00 0.00 0.01630 21000.00 10000.00 0.00 0.01741 21200.00 10000.00 0.00 0.01856 21400.00 10000.00 0.00 0.01978 21600.00 10000.00 0.00 0.02107 21800.00 10000.00 0.00 0.02230 22000.00 10000.00 0.00 0.02351 22200.00 10000.00 0.00 0.02466 22400.00 10000.00 0.00 0.02586 22600.00 10000.00 0.00 0.02708 22800.00 10000.00 0.00 0.02833 23000.00 10000.00 0.00 0.02979 23200.00 10000.00 0.00 0.03143 23400.00 10000.00 0.00 0.03311 23600.00 10000.00 0.00 0.03462 23800.00 10000.00 0.00 0.03615 24000.00 10000.00 0.00 0.03774 24200.00 10000.00 0.00 0.03913 24400.00 10000.00 0.00 0.04006 24600.00 10000.00 0.00 0.04045 24800.00 10000.00 0.00 0.04070 25000.00 10000.00 0.00 0.04087 25200.00 10000.00 0.00 0.04089 24800.00 10200.00 0.00 0.04102 25000.00 10200.00 0.00 0.04119 25200.00 10200.00 0.00 0.04121 24800.00 10400.00 0.00 0.04136 25000.00 10400.00 0.00 0.04153 25200.00 10400.00 0.00 0.04153 24800.00 10600.00 0.00 0.04167 25000.00 10600.00 0.00 0.04185 25200.00 10600.00 0.00 0.04187 24800.00 10800.00 0.00 0.04211 25000.00 10800.00 0.00 0.04233 25200.00 10800.00 0.00 0.04237 24800.00 11000.00 0.00 0.04263 25000.00 11000.00 0.00 0.04284 25200.00 11000.00 0.00 0.04286 24800.00 11200.00 0.00 0.04308 25000.00 11200.00 0.00 0.04326 25200.00 11200.00 0.00 0.04328 24800.00 11400.00 0.00 0.04355 25000.00 11400.00 0.00 0.04374 25200.00 11400.00 0.00 0.04383 25400.00 11400.00 0.00 0.04383 24800.00 11600.00 0.00 0.04396 25000.00 11600.00 0.00 0.04423 25200.00 11600.00 0.00 0.04436 24800.00 11800.00 0.00 0.04444 25000.00 11800.00 0.00 0.04473 25200.00 11800.00 0.00 0.04482 25000.00 12000.00 0.00 0.04509 25200.00 12000.00 0.00 0.04516 24800.00 12200.00 0.00 0.04524 25000.00 12200.00 0.00 0.04552 25200.00 12200.00 0.00 0.04561 24800.00 12400.00 0.00 0.04591 25000.00 12400.00 0.00 0.04620 25200.00 12400.00 0.00 0.04628 24800.00 12600.00 0.00 0.04653 25000.00 12600.00 0.00 0.04681 25200.00 12600.00 0.00 0.04686 24800.00 12800.00 0.00 0.04718 25000.00 12800.00 0.00 0.04744 25200.00 12800.00 0.00 0.04746 24800.00 13000.00 0.00 0.04774 25000.00 13000.00 0.00 0.04797 25200.00 13000.00 0.00 0.04799 24800.00 13200.00 0.00 0.04834 25000.00 13200.00 0.00 0.04857 25200.00 13200.00 0.00 0.04858 24800.00 13400.00 0.00 0.04899 25000.00 13400.00 0.00 0.04918 24800.00 13600.00 0.00 0.04953 25000.00 13600.00 0.00 0.04965 24800.00 13800.00 0.00 0.05011 25000.00 13800.00 0.00 0.05020 24600.00 14000.00 0.00 0.05028 24800.00 14000.00 0.00 0.05073 25000.00 14000.00 0.00 0.05081 24600.00 14200.00 0.00 0.05088 24800.00 14200.00 0.00 0.05135 25000.00 14200.00 0.00 0.05145 24600.00 14400.00 0.00 0.05149 24800.00 14400.00 0.00 0.05197 25000.00 14400.00 0.00 0.05208 24600.00 14600.00 0.00 0.05228 24800.00 14600.00 0.00 0.05275 25000.00 14600.00 0.00 0.05283 24600.00 14800.00 0.00 0.05307 24800.00 14800.00 0.00 0.05353 25000.00 14800.00 0.00 0.05358 24600.00 15000.00 0.00 0.05388 24800.00 15000.00 0.00 0.05431 25000.00 15000.00 0.00 0.05432 24600.00 15200.00 0.00 0.05472 24800.00 15200.00 0.00 0.05511 24600.00 15400.00 0.00 0.05556 24800.00 15400.00 0.00 0.05592 24600.00 15600.00 0.00 0.05650 24800.00 15600.00 0.00 0.05682 24600.00 15800.00 0.00 0.05745 24800.00 15800.00 0.00 0.05772 24400.00 16000.00 0.00 0.05780 24600.00 16000.00 0.00 0.05844 24800.00 16000.00 0.00 0.05868 24400.00 16200.00 0.00 0.05876 24600.00 16200.00 0.00 0.05939 24800.00 16200.00 0.00 0.05963 24400.00 16400.00 0.00 0.05981 24600.00 16400.00 0.00 0.06042 24800.00 16400.00 0.00 0.06063 24400.00 16600.00 0.00 0.06091 24600.00 16600.00 0.00 0.06149 24800.00 16600.00 0.00 0.06165 24400.00 16800.00 0.00 0.06200 24600.00 16800.00 0.00 0.06255 24800.00 16800.00 0.00 0.06270 24400.00 17000.00 0.00 0.06327 24600.00 17000.00 0.00 0.06381 24800.00 17000.00 0.00 0.06396 24400.00 17200.00 0.00 0.06459 24600.00 17200.00 0.00 0.06509 24800.00 17200.00 0.00 0.06521 24400.00 17400.00 0.00 0.06581 24600.00 17400.00 0.00 0.06628 24800.00 17400.00 0.00 0.06640 24400.00 17600.00 0.00 0.06699 24600.00 17600.00 0.00 0.06746 24800.00 17600.00 0.00 0.06759 24400.00 17800.00 0.00 0.06824 24600.00 17800.00 0.00 0.06873 24800.00 17800.00 0.00 0.06886 24400.00 18000.00 0.00 0.06944 24600.00 18000.00 0.00 0.06994 24800.00 18000.00 0.00 0.07006 24400.00 18200.00 0.00 0.07068 24600.00 18200.00 0.00 0.07117 24800.00 18200.00 0.00 0.07125 24400.00 18400.00 0.00 0.07200 24600.00 18400.00 0.00 0.07245 24800.00 18400.00 0.00 0.07250 24400.00 18600.00 0.00 0.07330 24600.00 18600.00 0.00 0.07371 24800.00 18600.00 0.00 0.07372 24400.00 18800.00 0.00 0.07460 24600.00 18800.00 0.00 0.07496 24800.00 18800.00 0.00 0.07499 24400.00 19000.00 0.00 0.07588 24600.00 19000.00 0.00 0.07624 24800.00 19000.00 0.00 0.07631 24400.00 19200.00 0.00 0.07734 24600.00 19200.00 0.00 0.07772 24800.00 19200.00 0.00 0.07783 24400.00 19400.00 0.00 0.07891 24600.00 19400.00 0.00 0.07932 24800.00 19400.00 0.00 0.07943 24200.00 19600.00 0.00 0.07945 24400.00 19600.00 0.00 0.08056 24600.00 19600.00 0.00 0.08097 24800.00 19600.00 0.00 0.08105 24200.00 19800.00 0.00 0.08106 24400.00 19800.00 0.00 0.08218 24600.00 19800.00 0.00 0.08256 24800.00 19800.00 0.00 0.08261 24200.00 20000.00 0.00 0.08267 24400.00 20000.00 0.00 0.08380 24600.00 20000.00 0.00 0.08419 24800.00 20000.00 0.00 0.08423 24200.00 20200.00 0.00 0.08438 24400.00 20200.00 0.00 0.08552 24600.00 20200.00 0.00 0.08590 24800.00 20200.00 0.00 0.08591 24200.00 20400.00 0.00 0.08612 24400.00 20400.00 0.00 0.08722 24600.00 20400.00 0.00 0.08755 24200.00 20600.00 0.00 0.08787 24400.00 20600.00 0.00 0.08891 24600.00 20600.00 0.00 0.08920 24200.00 20800.00 0.00 0.08945 24400.00 20800.00 0.00 0.09048 24600.00 20800.00 0.00 0.09076 24200.00 21000.00 0.00 0.09118 24400.00 21000.00 0.00 0.09223 24600.00 21000.00 0.00 0.09249 24200.00 21200.00 0.00 0.09300 24400.00 21200.00 0.00 0.09405 24600.00 21200.00 0.00 0.09423 24200.00 21400.00 0.00 0.09478 24400.00 21400.00 0.00 0.09581 24600.00 21400.00 0.00 0.09592 24200.00 21600.00 0.00 0.09651 24400.00 21600.00 0.00 0.09753 24600.00 21600.00 0.00 0.09758 24200.00 21800.00 0.00 0.09824 24400.00 21800.00 0.00 0.09921 24200.00 22000.00 0.00 0.10011 24400.00 22000.00 0.00 0.10103 24200.00 22200.00 0.00 0.10199 24400.00 22200.00 0.00 0.10284 24200.00 22400.00 0.00 0.10403 24400.00 22400.00 0.00 0.10482 24200.00 22600.00 0.00 0.10608 24400.00 22600.00 0.00 0.10683 24200.00 22800.00 0.00 0.10832 24400.00 22800.00 0.00 0.10907 24200.00 23000.00 0.00 0.11060 24400.00 23000.00 0.00 0.11132 24200.00 23200.00 0.00 0.11292 24400.00 23200.00 0.00 0.11356 24000.00 23400.00 0.00 0.11360 24200.00 23400.00 0.00 0.11539 24400.00 23400.00 0.00 0.11584 24000.00 23600.00 0.00 0.11594 24200.00 23600.00 0.00 0.11762 24400.00 23600.00 0.00 0.11784 24000.00 23800.00 0.00 0.11840 24200.00 23800.00 0.00 0.11987 24000.00 24000.00 0.00 0.12035 24200.00 24000.00 0.00 0.12162 24000.00 24200.00 0.00 0.12173 24200.00 24200.00 0.00 0.12269 24000.00 24400.00 25.00 0.12272 24000.00 24400.00 30.00 0.12282 24000.00 24400.00 35.00 0.12288 24000.00 24400.00 40.00 0.12288 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24076.01 24313.19 39.11 0.12312 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/028.ctf NX, NY, NZ: 512 512 1 MODE : real