CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/027.mrc Output diagnostic file name Micrographs_0601/027.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/027.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2100.000 7862.000 Mean, RMS : 4524.438 224.9706 TITLE 1: emi2mrc: 027.emi Sun Jan 05 13:48:26 2014 TITLE 2: kV 300, Mag 78000x, Def 25100, Spot 4, Int 55.10%, Obj 88.05%, Dif 73.67% TITLE 3: X 140.796, Y 312.636, Z -101.718, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00760 10200.00 10000.00 0.00 -0.00755 10400.00 10000.00 0.00 -0.00744 10600.00 10000.00 0.00 -0.00733 10800.00 10000.00 0.00 -0.00720 11000.00 10000.00 0.00 -0.00713 11200.00 10000.00 0.00 -0.00709 11800.00 10000.00 0.00 -0.00703 12000.00 10000.00 0.00 -0.00692 12200.00 10000.00 0.00 -0.00665 12400.00 10000.00 0.00 -0.00635 12600.00 10000.00 0.00 -0.00626 12800.00 10000.00 0.00 -0.00605 13000.00 10000.00 0.00 -0.00579 13200.00 10000.00 0.00 -0.00560 13400.00 10000.00 0.00 -0.00542 13600.00 10000.00 0.00 -0.00513 13800.00 10000.00 0.00 -0.00483 14000.00 10000.00 0.00 -0.00457 14200.00 10000.00 0.00 -0.00426 14400.00 10000.00 0.00 -0.00398 14600.00 10000.00 0.00 -0.00368 14800.00 10000.00 0.00 -0.00331 15000.00 10000.00 0.00 -0.00304 15200.00 10000.00 0.00 -0.00276 15400.00 10000.00 0.00 -0.00242 15600.00 10000.00 0.00 -0.00203 15800.00 10000.00 0.00 -0.00156 16000.00 10000.00 0.00 -0.00104 16200.00 10000.00 0.00 -0.00052 16400.00 10000.00 0.00 -0.00002 16600.00 10000.00 0.00 0.00048 16800.00 10000.00 0.00 0.00102 17000.00 10000.00 0.00 0.00161 17200.00 10000.00 0.00 0.00241 17400.00 10000.00 0.00 0.00340 17600.00 10000.00 0.00 0.00429 17800.00 10000.00 0.00 0.00506 18000.00 10000.00 0.00 0.00584 18200.00 10000.00 0.00 0.00661 18400.00 10000.00 0.00 0.00733 18600.00 10000.00 0.00 0.00788 18800.00 10000.00 0.00 0.00840 19000.00 10000.00 0.00 0.00910 19200.00 10000.00 0.00 0.01012 19400.00 10000.00 0.00 0.01132 19600.00 10000.00 0.00 0.01243 19800.00 10000.00 0.00 0.01347 20000.00 10000.00 0.00 0.01444 20200.00 10000.00 0.00 0.01544 20400.00 10000.00 0.00 0.01636 20600.00 10000.00 0.00 0.01728 20800.00 10000.00 0.00 0.01821 21000.00 10000.00 0.00 0.01908 21200.00 10000.00 0.00 0.02019 21400.00 10000.00 0.00 0.02143 21600.00 10000.00 0.00 0.02266 21800.00 10000.00 0.00 0.02387 22000.00 10000.00 0.00 0.02506 22200.00 10000.00 0.00 0.02629 22400.00 10000.00 0.00 0.02749 22600.00 10000.00 0.00 0.02882 22800.00 10000.00 0.00 0.03033 23000.00 10000.00 0.00 0.03191 23200.00 10000.00 0.00 0.03365 23400.00 10000.00 0.00 0.03540 23600.00 10000.00 0.00 0.03709 23800.00 10000.00 0.00 0.03883 24000.00 10000.00 0.00 0.04034 24200.00 10000.00 0.00 0.04142 24400.00 10000.00 0.00 0.04204 24600.00 10000.00 0.00 0.04233 24800.00 10000.00 0.00 0.04250 24600.00 10200.00 0.00 0.04274 24800.00 10200.00 0.00 0.04285 24600.00 10400.00 0.00 0.04299 24800.00 10400.00 0.00 0.04304 24600.00 10600.00 0.00 0.04318 24800.00 10600.00 0.00 0.04324 24400.00 10800.00 0.00 0.04328 24600.00 10800.00 0.00 0.04348 24800.00 10800.00 0.00 0.04359 24400.00 11000.00 0.00 0.04375 24600.00 11000.00 0.00 0.04398 24800.00 11000.00 0.00 0.04410 24400.00 11200.00 0.00 0.04422 24600.00 11200.00 0.00 0.04446 24800.00 11200.00 0.00 0.04455 24400.00 11400.00 0.00 0.04465 24600.00 11400.00 0.00 0.04487 24800.00 11400.00 0.00 0.04490 24400.00 11600.00 0.00 0.04502 24600.00 11600.00 0.00 0.04520 24800.00 11600.00 0.00 0.04521 24400.00 11800.00 0.00 0.04549 24600.00 11800.00 0.00 0.04566 24800.00 11800.00 0.00 0.04566 24400.00 12000.00 0.00 0.04598 24600.00 12000.00 0.00 0.04614 24800.00 12000.00 0.00 0.04615 24400.00 12200.00 0.00 0.04647 24600.00 12200.00 0.00 0.04664 24800.00 12200.00 0.00 0.04667 24400.00 12400.00 0.00 0.04701 24600.00 12400.00 0.00 0.04716 24800.00 12400.00 0.00 0.04721 24400.00 12600.00 0.00 0.04737 24600.00 12600.00 0.00 0.04753 24800.00 12600.00 0.00 0.04762 25000.00 12600.00 0.00 0.04764 24400.00 12800.00 0.00 0.04773 24600.00 12800.00 0.00 0.04797 24800.00 12800.00 0.00 0.04814 25000.00 12800.00 0.00 0.04816 24400.00 13000.00 0.00 0.04829 24600.00 13000.00 0.00 0.04862 24800.00 13000.00 0.00 0.04880 24400.00 13200.00 0.00 0.04894 24600.00 13200.00 0.00 0.04929 24800.00 13200.00 0.00 0.04942 24400.00 13400.00 0.00 0.04957 24600.00 13400.00 0.00 0.04990 24800.00 13400.00 0.00 0.05000 24400.00 13600.00 0.00 0.05034 24600.00 13600.00 0.00 0.05063 24800.00 13600.00 0.00 0.05068 24400.00 13800.00 0.00 0.05096 24600.00 13800.00 0.00 0.05120 24800.00 13800.00 0.00 0.05123 24400.00 14000.00 0.00 0.05145 24600.00 14000.00 0.00 0.05170 24800.00 14000.00 0.00 0.05177 24400.00 14200.00 0.00 0.05214 24600.00 14200.00 0.00 0.05242 24800.00 14200.00 0.00 0.05249 24400.00 14400.00 0.00 0.05286 24600.00 14400.00 0.00 0.05314 24800.00 14400.00 0.00 0.05317 24400.00 14600.00 0.00 0.05370 24600.00 14600.00 0.00 0.05394 24800.00 14600.00 0.00 0.05395 24400.00 14800.00 0.00 0.05452 24600.00 14800.00 0.00 0.05474 24800.00 14800.00 0.00 0.05476 25000.00 14800.00 0.00 0.05477 25200.00 14800.00 0.00 0.05478 24400.00 15000.00 0.00 0.05536 24600.00 15000.00 0.00 0.05559 24800.00 15000.00 0.00 0.05564 25000.00 15000.00 0.00 0.05565 24400.00 15200.00 0.00 0.05624 24600.00 15200.00 0.00 0.05647 24800.00 15200.00 0.00 0.05649 24400.00 15400.00 0.00 0.05708 24600.00 15400.00 0.00 0.05729 24200.00 15600.00 0.00 0.05739 24400.00 15600.00 0.00 0.05800 24600.00 15600.00 0.00 0.05820 24200.00 15800.00 0.00 0.05831 24400.00 15800.00 0.00 0.05893 24600.00 15800.00 0.00 0.05911 24200.00 16000.00 0.00 0.05923 24400.00 16000.00 0.00 0.05986 24600.00 16000.00 0.00 0.06002 24200.00 16200.00 0.00 0.06019 24400.00 16200.00 0.00 0.06080 24600.00 16200.00 0.00 0.06093 24200.00 16400.00 0.00 0.06128 24400.00 16400.00 0.00 0.06186 24600.00 16400.00 0.00 0.06198 24200.00 16600.00 0.00 0.06231 24400.00 16600.00 0.00 0.06286 24600.00 16600.00 0.00 0.06298 24200.00 16800.00 0.00 0.06327 24400.00 16800.00 0.00 0.06383 24600.00 16800.00 0.00 0.06393 24200.00 17000.00 0.00 0.06425 24400.00 17000.00 0.00 0.06483 24600.00 17000.00 0.00 0.06490 24200.00 17200.00 0.00 0.06525 24400.00 17200.00 0.00 0.06583 24600.00 17200.00 0.00 0.06589 24200.00 17400.00 0.00 0.06640 24400.00 17400.00 0.00 0.06697 24600.00 17400.00 0.00 0.06704 24200.00 17600.00 0.00 0.06762 24400.00 17600.00 0.00 0.06822 24600.00 17600.00 0.00 0.06830 24200.00 17800.00 0.00 0.06901 24400.00 17800.00 0.00 0.06961 24600.00 17800.00 0.00 0.06969 24200.00 18000.00 0.00 0.07039 24400.00 18000.00 0.00 0.07099 24600.00 18000.00 0.00 0.07104 24200.00 18200.00 0.00 0.07183 24400.00 18200.00 0.00 0.07241 24600.00 18200.00 0.00 0.07245 24200.00 18400.00 0.00 0.07331 24400.00 18400.00 0.00 0.07387 24600.00 18400.00 0.00 0.07389 24200.00 18600.00 0.00 0.07479 24400.00 18600.00 0.00 0.07530 24200.00 18800.00 0.00 0.07626 24400.00 18800.00 0.00 0.07675 24200.00 19000.00 0.00 0.07766 24400.00 19000.00 0.00 0.07814 24200.00 19200.00 0.00 0.07926 24400.00 19200.00 0.00 0.07971 24200.00 19400.00 0.00 0.08084 24400.00 19400.00 0.00 0.08125 24000.00 19600.00 0.00 0.08135 24200.00 19600.00 0.00 0.08249 24400.00 19600.00 0.00 0.08291 24000.00 19800.00 0.00 0.08311 24200.00 19800.00 0.00 0.08423 24400.00 19800.00 0.00 0.08465 24000.00 20000.00 0.00 0.08485 24200.00 20000.00 0.00 0.08593 24400.00 20000.00 0.00 0.08631 24000.00 20200.00 0.00 0.08647 24200.00 20200.00 0.00 0.08754 24400.00 20200.00 0.00 0.08788 24000.00 20400.00 0.00 0.08807 24200.00 20400.00 0.00 0.08913 24400.00 20400.00 0.00 0.08944 24000.00 20600.00 0.00 0.08987 24200.00 20600.00 0.00 0.09091 24400.00 20600.00 0.00 0.09117 24000.00 20800.00 0.00 0.09159 24200.00 20800.00 0.00 0.09260 24400.00 20800.00 0.00 0.09283 24000.00 21000.00 0.00 0.09343 24200.00 21000.00 0.00 0.09440 24400.00 21000.00 0.00 0.09459 24000.00 21200.00 0.00 0.09533 24200.00 21200.00 0.00 0.09623 24400.00 21200.00 0.00 0.09638 24000.00 21400.00 0.00 0.09729 24200.00 21400.00 0.00 0.09815 24400.00 21400.00 0.00 0.09827 24000.00 21600.00 0.00 0.09938 24200.00 21600.00 0.00 0.10024 24400.00 21600.00 0.00 0.10034 24000.00 21800.00 0.00 0.10145 24200.00 21800.00 0.00 0.10231 24400.00 21800.00 0.00 0.10237 24000.00 22000.00 0.00 0.10361 24200.00 22000.00 0.00 0.10444 24000.00 22200.00 0.00 0.10572 24200.00 22200.00 0.00 0.10653 24000.00 22400.00 0.00 0.10802 24200.00 22400.00 0.00 0.10876 24000.00 22600.00 0.00 0.11036 24200.00 22600.00 0.00 0.11101 23800.00 22800.00 0.00 0.11112 24000.00 22800.00 0.00 0.11287 24200.00 22800.00 0.00 0.11337 23800.00 23000.00 0.00 0.11368 24000.00 23000.00 0.00 0.11538 24200.00 23000.00 0.00 0.11575 23800.00 23200.00 0.00 0.11633 24000.00 23200.00 0.00 0.11795 24200.00 23200.00 0.00 0.11814 23800.00 23400.00 0.00 0.11901 24000.00 23400.00 0.00 0.12047 23800.00 23600.00 0.00 0.12136 24000.00 23600.00 0.00 0.12262 23800.00 23800.00 0.00 0.12389 24000.00 23800.00 0.00 0.12467 23800.00 24000.00 0.00 0.12509 24000.00 24000.00 0.00 0.12514 23800.00 24000.00 5.00 0.12520 23800.00 24000.00 10.00 0.12530 23800.00 24000.00 15.00 0.12539 23800.00 24000.00 20.00 0.12547 23800.00 24000.00 25.00 0.12553 23800.00 24000.00 30.00 0.12557 23800.00 24000.00 35.00 0.12559 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23843.53 24055.18 37.34 0.12577 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/027.ctf NX, NY, NZ: 512 512 1 MODE : real