CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/025.mrc Output diagnostic file name Micrographs_0601/025.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/025.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2170.000 8018.000 Mean, RMS : 4505.352 219.6912 TITLE 1: emi2mrc: 025.emi Sun Jan 05 13:42:18 2014 TITLE 2: kV 300, Mag 78000x, Def 25600, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 347.414, Y 282.081, Z -105.479, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00749 10200.00 10000.00 0.00 -0.00746 10400.00 10000.00 0.00 -0.00735 10600.00 10000.00 0.00 -0.00730 10800.00 10000.00 0.00 -0.00719 11000.00 10000.00 0.00 -0.00699 11200.00 10000.00 0.00 -0.00683 11400.00 10000.00 0.00 -0.00654 11600.00 10000.00 0.00 -0.00609 11800.00 10000.00 0.00 -0.00573 12000.00 10000.00 0.00 -0.00533 12200.00 10000.00 0.00 -0.00502 12400.00 10000.00 0.00 -0.00468 12600.00 10000.00 0.00 -0.00412 12800.00 10000.00 0.00 -0.00364 13000.00 10000.00 0.00 -0.00320 13200.00 10000.00 0.00 -0.00272 13400.00 10000.00 0.00 -0.00227 13600.00 10000.00 0.00 -0.00175 13800.00 10000.00 0.00 -0.00107 14000.00 10000.00 0.00 -0.00043 14200.00 10000.00 0.00 0.00011 14400.00 10000.00 0.00 0.00078 14600.00 10000.00 0.00 0.00148 14800.00 10000.00 0.00 0.00226 15000.00 10000.00 0.00 0.00310 15200.00 10000.00 0.00 0.00385 15400.00 10000.00 0.00 0.00472 15600.00 10000.00 0.00 0.00548 15800.00 10000.00 0.00 0.00626 16000.00 10000.00 0.00 0.00730 16200.00 10000.00 0.00 0.00831 16400.00 10000.00 0.00 0.00926 16600.00 10000.00 0.00 0.01025 16800.00 10000.00 0.00 0.01132 17000.00 10000.00 0.00 0.01251 17200.00 10000.00 0.00 0.01370 17400.00 10000.00 0.00 0.01501 17600.00 10000.00 0.00 0.01635 17800.00 10000.00 0.00 0.01757 18000.00 10000.00 0.00 0.01884 18200.00 10000.00 0.00 0.02002 18400.00 10000.00 0.00 0.02115 18600.00 10000.00 0.00 0.02242 18800.00 10000.00 0.00 0.02383 19000.00 10000.00 0.00 0.02530 19200.00 10000.00 0.00 0.02694 19400.00 10000.00 0.00 0.02870 19600.00 10000.00 0.00 0.03043 19800.00 10000.00 0.00 0.03224 20000.00 10000.00 0.00 0.03418 20200.00 10000.00 0.00 0.03602 20400.00 10000.00 0.00 0.03779 20600.00 10000.00 0.00 0.03979 20800.00 10000.00 0.00 0.04199 21000.00 10000.00 0.00 0.04424 21200.00 10000.00 0.00 0.04594 21400.00 10000.00 0.00 0.04683 21600.00 10000.00 0.00 0.04726 21800.00 10000.00 0.00 0.04731 21400.00 10200.00 0.00 0.04750 21600.00 10200.00 0.00 0.04787 21800.00 10200.00 0.00 0.04788 21400.00 10400.00 0.00 0.04815 21600.00 10400.00 0.00 0.04848 21400.00 10600.00 0.00 0.04886 21600.00 10600.00 0.00 0.04918 21800.00 10600.00 0.00 0.04920 22000.00 10600.00 0.00 0.04922 21400.00 10800.00 0.00 0.04966 21600.00 10800.00 0.00 0.05001 21800.00 10800.00 0.00 0.05003 21400.00 11000.00 0.00 0.05047 21600.00 11000.00 0.00 0.05080 21400.00 11200.00 0.00 0.05116 21600.00 11200.00 0.00 0.05148 21800.00 11200.00 0.00 0.05149 22000.00 11200.00 0.00 0.05150 21400.00 11400.00 0.00 0.05186 21600.00 11400.00 0.00 0.05221 21800.00 11400.00 0.00 0.05224 22000.00 11400.00 0.00 0.05226 21400.00 11600.00 0.00 0.05248 21600.00 11600.00 0.00 0.05284 21800.00 11600.00 0.00 0.05286 21400.00 11800.00 0.00 0.05311 21600.00 11800.00 0.00 0.05348 21800.00 11800.00 0.00 0.05348 21400.00 12000.00 0.00 0.05382 21600.00 12000.00 0.00 0.05418 21400.00 12200.00 0.00 0.05461 21600.00 12200.00 0.00 0.05493 21400.00 12400.00 0.00 0.05538 21600.00 12400.00 0.00 0.05564 21400.00 12600.00 0.00 0.05609 21600.00 12600.00 0.00 0.05629 21400.00 12800.00 0.00 0.05690 21600.00 12800.00 0.00 0.05703 21400.00 13000.00 0.00 0.05772 21600.00 13000.00 0.00 0.05783 21200.00 13200.00 0.00 0.05791 21400.00 13200.00 0.00 0.05868 21600.00 13200.00 0.00 0.05880 21200.00 13400.00 0.00 0.05900 21400.00 13400.00 0.00 0.05976 21600.00 13400.00 0.00 0.05990 21200.00 13600.00 0.00 0.06019 21400.00 13600.00 0.00 0.06094 21600.00 13600.00 0.00 0.06109 21800.00 13600.00 0.00 0.06110 21200.00 13800.00 0.00 0.06135 21400.00 13800.00 0.00 0.06210 21600.00 13800.00 0.00 0.06224 21800.00 13800.00 0.00 0.06224 21200.00 14000.00 0.00 0.06259 21400.00 14000.00 0.00 0.06331 21600.00 14000.00 0.00 0.06345 21800.00 14000.00 0.00 0.06346 21200.00 14200.00 0.00 0.06384 21400.00 14200.00 0.00 0.06455 21600.00 14200.00 0.00 0.06471 21800.00 14200.00 0.00 0.06473 21200.00 14400.00 0.00 0.06494 21400.00 14400.00 0.00 0.06566 21600.00 14400.00 0.00 0.06583 21800.00 14400.00 0.00 0.06586 21200.00 14600.00 0.00 0.06627 21400.00 14600.00 0.00 0.06698 21600.00 14600.00 0.00 0.06714 21200.00 14800.00 0.00 0.06761 21400.00 14800.00 0.00 0.06827 21600.00 14800.00 0.00 0.06840 21200.00 15000.00 0.00 0.06905 21400.00 15000.00 0.00 0.06966 21600.00 15000.00 0.00 0.06979 21200.00 15200.00 0.00 0.07037 21400.00 15200.00 0.00 0.07100 21600.00 15200.00 0.00 0.07116 21200.00 15400.00 0.00 0.07168 21400.00 15400.00 0.00 0.07235 21600.00 15400.00 0.00 0.07252 21200.00 15600.00 0.00 0.07331 21400.00 15600.00 0.00 0.07402 21600.00 15600.00 0.00 0.07417 21200.00 15800.00 0.00 0.07492 21400.00 15800.00 0.00 0.07563 21600.00 15800.00 0.00 0.07574 21200.00 16000.00 0.00 0.07661 21400.00 16000.00 0.00 0.07731 21600.00 16000.00 0.00 0.07740 21200.00 16200.00 0.00 0.07825 21400.00 16200.00 0.00 0.07893 21600.00 16200.00 0.00 0.07896 21200.00 16400.00 0.00 0.07996 21400.00 16400.00 0.00 0.08060 21200.00 16600.00 0.00 0.08174 21400.00 16600.00 0.00 0.08232 21200.00 16800.00 0.00 0.08351 21400.00 16800.00 0.00 0.08403 21200.00 17000.00 0.00 0.08539 21400.00 17000.00 0.00 0.08589 21200.00 17200.00 0.00 0.08738 21400.00 17200.00 0.00 0.08782 21200.00 17400.00 0.00 0.08936 21400.00 17400.00 0.00 0.08970 21200.00 17600.00 0.00 0.09116 21400.00 17600.00 0.00 0.09143 21000.00 17800.00 0.00 0.09168 21200.00 17800.00 0.00 0.09324 21400.00 17800.00 0.00 0.09350 21000.00 18000.00 0.00 0.09376 21200.00 18000.00 0.00 0.09533 21400.00 18000.00 0.00 0.09553 21000.00 18200.00 0.00 0.09589 21200.00 18200.00 0.00 0.09744 21400.00 18200.00 0.00 0.09756 21000.00 18400.00 0.00 0.09807 21200.00 18400.00 0.00 0.09957 21400.00 18400.00 0.00 0.09961 21000.00 18600.00 0.00 0.10020 21200.00 18600.00 0.00 0.10166 21000.00 18800.00 0.00 0.10255 21200.00 18800.00 0.00 0.10399 21000.00 19000.00 0.00 0.10498 21200.00 19000.00 0.00 0.10639 21000.00 19200.00 0.00 0.10772 21200.00 19200.00 0.00 0.10908 21000.00 19400.00 0.00 0.11036 21200.00 19400.00 0.00 0.11167 21000.00 19600.00 0.00 0.11320 21200.00 19600.00 0.00 0.11446 21000.00 19800.00 0.00 0.11613 21200.00 19800.00 0.00 0.11727 21000.00 20000.00 0.00 0.11892 21200.00 20000.00 0.00 0.11984 21000.00 20200.00 0.00 0.12178 21200.00 20200.00 0.00 0.12251 21000.00 20400.00 0.00 0.12453 21200.00 20400.00 0.00 0.12507 20800.00 20600.00 0.00 0.12553 21000.00 20600.00 0.00 0.12753 21200.00 20600.00 0.00 0.12777 20800.00 20800.00 0.00 0.12840 21000.00 20800.00 0.00 0.13020 20800.00 21000.00 0.00 0.13077 21000.00 21000.00 0.00 0.13217 21000.00 21200.00 10.00 0.13219 21000.00 21200.00 15.00 0.13235 21000.00 21200.00 20.00 0.13249 21000.00 21200.00 25.00 0.13259 21000.00 21200.00 30.00 0.13267 21000.00 21200.00 35.00 0.13271 21000.00 21200.00 40.00 0.13271 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20934.71 21162.12 36.70 0.13302 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/025.ctf NX, NY, NZ: 512 512 1 MODE : real