CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/023.mrc Output diagnostic file name Micrographs_0601/023.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/023.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2458.000 7678.000 Mean, RMS : 4499.428 222.3157 TITLE 1: emi2mrc: 023.emi Sun Jan 05 13:40:57 2014 TITLE 2: kV 300, Mag 78000x, Def 25600, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 342.267, Y 282.874, Z -105.479, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00166 10200.00 10000.00 0.00 0.00168 10800.00 10000.00 0.00 0.00176 11000.00 10000.00 0.00 0.00209 11200.00 10000.00 0.00 0.00226 11400.00 10000.00 0.00 0.00241 11600.00 10000.00 0.00 0.00256 11800.00 10000.00 0.00 0.00266 12000.00 10000.00 0.00 0.00294 12200.00 10000.00 0.00 0.00333 12400.00 10000.00 0.00 0.00378 12600.00 10000.00 0.00 0.00418 12800.00 10000.00 0.00 0.00453 13000.00 10000.00 0.00 0.00494 13200.00 10000.00 0.00 0.00534 13400.00 10000.00 0.00 0.00576 13600.00 10000.00 0.00 0.00625 13800.00 10000.00 0.00 0.00680 14000.00 10000.00 0.00 0.00742 14200.00 10000.00 0.00 0.00819 14400.00 10000.00 0.00 0.00895 14600.00 10000.00 0.00 0.00970 14800.00 10000.00 0.00 0.01050 15000.00 10000.00 0.00 0.01128 15200.00 10000.00 0.00 0.01194 15400.00 10000.00 0.00 0.01265 15600.00 10000.00 0.00 0.01355 15800.00 10000.00 0.00 0.01447 16000.00 10000.00 0.00 0.01542 16200.00 10000.00 0.00 0.01643 16400.00 10000.00 0.00 0.01755 16600.00 10000.00 0.00 0.01878 16800.00 10000.00 0.00 0.01989 17000.00 10000.00 0.00 0.02108 17200.00 10000.00 0.00 0.02239 17400.00 10000.00 0.00 0.02367 17600.00 10000.00 0.00 0.02501 17800.00 10000.00 0.00 0.02635 18000.00 10000.00 0.00 0.02761 18200.00 10000.00 0.00 0.02898 18400.00 10000.00 0.00 0.03043 18600.00 10000.00 0.00 0.03181 18800.00 10000.00 0.00 0.03331 19000.00 10000.00 0.00 0.03487 19200.00 10000.00 0.00 0.03629 19400.00 10000.00 0.00 0.03758 19600.00 10000.00 0.00 0.03887 19800.00 10000.00 0.00 0.04028 20000.00 10000.00 0.00 0.04183 20200.00 10000.00 0.00 0.04356 20400.00 10000.00 0.00 0.04551 20600.00 10000.00 0.00 0.04770 20800.00 10000.00 0.00 0.04988 21000.00 10000.00 0.00 0.05209 21200.00 10000.00 0.00 0.05420 21400.00 10000.00 0.00 0.05568 21600.00 10000.00 0.00 0.05653 21800.00 10000.00 0.00 0.05696 22000.00 10000.00 0.00 0.05715 21800.00 10200.00 0.00 0.05743 22000.00 10200.00 0.00 0.05762 21800.00 10400.00 0.00 0.05790 22000.00 10400.00 0.00 0.05809 21600.00 10600.00 0.00 0.05810 21800.00 10600.00 0.00 0.05854 22000.00 10600.00 0.00 0.05872 21600.00 10800.00 0.00 0.05892 21800.00 10800.00 0.00 0.05934 22000.00 10800.00 0.00 0.05949 21600.00 11000.00 0.00 0.05978 21800.00 11000.00 0.00 0.06014 22000.00 11000.00 0.00 0.06026 21600.00 11200.00 0.00 0.06046 21800.00 11200.00 0.00 0.06077 22000.00 11200.00 0.00 0.06090 21600.00 11400.00 0.00 0.06097 21800.00 11400.00 0.00 0.06130 22000.00 11400.00 0.00 0.06146 21600.00 11600.00 0.00 0.06149 21800.00 11600.00 0.00 0.06188 22000.00 11600.00 0.00 0.06205 21600.00 11800.00 0.00 0.06226 21800.00 11800.00 0.00 0.06267 22000.00 11800.00 0.00 0.06281 21600.00 12000.00 0.00 0.06298 21800.00 12000.00 0.00 0.06340 22000.00 12000.00 0.00 0.06351 21600.00 12200.00 0.00 0.06388 21800.00 12200.00 0.00 0.06430 22000.00 12200.00 0.00 0.06438 21600.00 12400.00 0.00 0.06492 21800.00 12400.00 0.00 0.06529 21600.00 12600.00 0.00 0.06570 21800.00 12600.00 0.00 0.06598 21600.00 12800.00 0.00 0.06654 21800.00 12800.00 0.00 0.06677 21600.00 13000.00 0.00 0.06726 21800.00 13000.00 0.00 0.06749 22000.00 13000.00 0.00 0.06752 21600.00 13200.00 0.00 0.06817 21800.00 13200.00 0.00 0.06843 22000.00 13200.00 0.00 0.06847 21400.00 13400.00 0.00 0.06860 21600.00 13400.00 0.00 0.06934 21800.00 13400.00 0.00 0.06960 21400.00 13600.00 0.00 0.06975 21600.00 13600.00 0.00 0.07047 21800.00 13600.00 0.00 0.07072 21400.00 13800.00 0.00 0.07092 21600.00 13800.00 0.00 0.07162 21800.00 13800.00 0.00 0.07185 21400.00 14000.00 0.00 0.07202 21600.00 14000.00 0.00 0.07269 21800.00 14000.00 0.00 0.07288 21400.00 14200.00 0.00 0.07316 21600.00 14200.00 0.00 0.07381 21800.00 14200.00 0.00 0.07400 21400.00 14400.00 0.00 0.07431 21600.00 14400.00 0.00 0.07498 21800.00 14400.00 0.00 0.07518 21400.00 14600.00 0.00 0.07552 21600.00 14600.00 0.00 0.07620 21800.00 14600.00 0.00 0.07642 21400.00 14800.00 0.00 0.07676 21600.00 14800.00 0.00 0.07745 21800.00 14800.00 0.00 0.07766 21400.00 15000.00 0.00 0.07809 21600.00 15000.00 0.00 0.07876 21800.00 15000.00 0.00 0.07896 21400.00 15200.00 0.00 0.07945 21600.00 15200.00 0.00 0.08014 21800.00 15200.00 0.00 0.08031 21400.00 15400.00 0.00 0.08088 21600.00 15400.00 0.00 0.08157 21800.00 15400.00 0.00 0.08173 21400.00 15600.00 0.00 0.08233 21600.00 15600.00 0.00 0.08301 21800.00 15600.00 0.00 0.08316 21400.00 15800.00 0.00 0.08379 21600.00 15800.00 0.00 0.08448 21800.00 15800.00 0.00 0.08463 21400.00 16000.00 0.00 0.08550 21600.00 16000.00 0.00 0.08619 21800.00 16000.00 0.00 0.08632 21400.00 16200.00 0.00 0.08713 21600.00 16200.00 0.00 0.08781 21800.00 16200.00 0.00 0.08794 21400.00 16400.00 0.00 0.08888 21600.00 16400.00 0.00 0.08956 21800.00 16400.00 0.00 0.08968 21400.00 16600.00 0.00 0.09067 21600.00 16600.00 0.00 0.09134 21800.00 16600.00 0.00 0.09145 21400.00 16800.00 0.00 0.09241 21600.00 16800.00 0.00 0.09308 21800.00 16800.00 0.00 0.09318 21400.00 17000.00 0.00 0.09431 21600.00 17000.00 0.00 0.09497 21800.00 17000.00 0.00 0.09504 21400.00 17200.00 0.00 0.09620 21600.00 17200.00 0.00 0.09685 21400.00 17400.00 0.00 0.09815 21600.00 17400.00 0.00 0.09876 21400.00 17600.00 0.00 0.10004 21600.00 17600.00 0.00 0.10064 21400.00 17800.00 0.00 0.10205 21600.00 17800.00 0.00 0.10262 21400.00 18000.00 0.00 0.10408 21600.00 18000.00 0.00 0.10461 21200.00 18200.00 0.00 0.10466 21400.00 18200.00 0.00 0.10616 21600.00 18200.00 0.00 0.10663 21200.00 18400.00 0.00 0.10680 21400.00 18400.00 0.00 0.10829 21600.00 18400.00 0.00 0.10869 21200.00 18600.00 0.00 0.10907 21400.00 18600.00 0.00 0.11056 21600.00 18600.00 0.00 0.11086 21200.00 18800.00 0.00 0.11155 21400.00 18800.00 0.00 0.11300 21600.00 18800.00 0.00 0.11319 21200.00 19000.00 0.00 0.11390 21400.00 19000.00 0.00 0.11528 21600.00 19000.00 0.00 0.11539 21200.00 19200.00 0.00 0.11648 21400.00 19200.00 0.00 0.11779 21600.00 19200.00 0.00 0.11783 21200.00 19400.00 0.00 0.11916 21400.00 19400.00 0.00 0.12040 21200.00 19600.00 0.00 0.12197 21400.00 19600.00 0.00 0.12310 21200.00 19800.00 0.00 0.12471 21400.00 19800.00 0.00 0.12569 21200.00 20000.00 0.00 0.12753 21400.00 20000.00 0.00 0.12834 21200.00 20200.00 0.00 0.13060 21400.00 20200.00 0.00 0.13121 21200.00 20400.00 0.00 0.13356 21400.00 20400.00 0.00 0.13391 21000.00 20600.00 0.00 0.13417 21200.00 20600.00 0.00 0.13670 21000.00 20800.00 0.00 0.13729 21200.00 20800.00 0.00 0.13963 21000.00 21000.00 0.00 0.14068 21200.00 21000.00 0.00 0.14255 21000.00 21200.00 0.00 0.14302 21200.00 21200.00 0.00 0.14396 21000.00 21400.00 35.00 0.14398 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21097.78 21290.16 34.54 0.14443 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/023.ctf NX, NY, NZ: 512 512 1 MODE : real