CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/022.mrc Output diagnostic file name Micrographs_0601/022.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/022.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2424.000 7884.000 Mean, RMS : 4462.831 216.5978 TITLE 1: emi2mrc: 022.emi Sun Jan 05 13:40:12 2014 TITLE 2: kV 300, Mag 78000x, Def 25600, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 339.589, Y 283.199, Z -105.479, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00197 10800.00 10000.00 0.00 -0.00195 11000.00 10000.00 0.00 -0.00183 11200.00 10000.00 0.00 -0.00167 11400.00 10000.00 0.00 -0.00139 11600.00 10000.00 0.00 -0.00112 11800.00 10000.00 0.00 -0.00078 12000.00 10000.00 0.00 -0.00046 12200.00 10000.00 0.00 -0.00015 12400.00 10000.00 0.00 0.00031 12600.00 10000.00 0.00 0.00071 12800.00 10000.00 0.00 0.00122 13000.00 10000.00 0.00 0.00186 13200.00 10000.00 0.00 0.00249 13400.00 10000.00 0.00 0.00322 13600.00 10000.00 0.00 0.00379 13800.00 10000.00 0.00 0.00433 14000.00 10000.00 0.00 0.00489 14200.00 10000.00 0.00 0.00529 14400.00 10000.00 0.00 0.00569 14600.00 10000.00 0.00 0.00619 14800.00 10000.00 0.00 0.00684 15000.00 10000.00 0.00 0.00764 15200.00 10000.00 0.00 0.00836 15400.00 10000.00 0.00 0.00913 15600.00 10000.00 0.00 0.01018 15800.00 10000.00 0.00 0.01119 16000.00 10000.00 0.00 0.01216 16200.00 10000.00 0.00 0.01323 16400.00 10000.00 0.00 0.01432 16600.00 10000.00 0.00 0.01541 16800.00 10000.00 0.00 0.01640 17000.00 10000.00 0.00 0.01745 17200.00 10000.00 0.00 0.01848 17400.00 10000.00 0.00 0.01949 17600.00 10000.00 0.00 0.02067 17800.00 10000.00 0.00 0.02187 18000.00 10000.00 0.00 0.02317 18200.00 10000.00 0.00 0.02448 18400.00 10000.00 0.00 0.02569 18600.00 10000.00 0.00 0.02691 18800.00 10000.00 0.00 0.02816 19000.00 10000.00 0.00 0.02936 19200.00 10000.00 0.00 0.03059 19400.00 10000.00 0.00 0.03202 19600.00 10000.00 0.00 0.03364 19800.00 10000.00 0.00 0.03529 20000.00 10000.00 0.00 0.03691 20200.00 10000.00 0.00 0.03863 20400.00 10000.00 0.00 0.04032 20600.00 10000.00 0.00 0.04213 20800.00 10000.00 0.00 0.04408 21000.00 10000.00 0.00 0.04599 21200.00 10000.00 0.00 0.04799 21400.00 10000.00 0.00 0.04997 21600.00 10000.00 0.00 0.05171 21800.00 10000.00 0.00 0.05284 22000.00 10000.00 0.00 0.05323 22200.00 10000.00 0.00 0.05332 21800.00 10200.00 0.00 0.05339 22000.00 10200.00 0.00 0.05375 22200.00 10200.00 0.00 0.05386 22400.00 10200.00 0.00 0.05388 21800.00 10400.00 0.00 0.05392 22000.00 10400.00 0.00 0.05428 22200.00 10400.00 0.00 0.05445 22400.00 10400.00 0.00 0.05453 22000.00 10600.00 0.00 0.05481 22200.00 10600.00 0.00 0.05508 22400.00 10600.00 0.00 0.05516 22000.00 10800.00 0.00 0.05549 22200.00 10800.00 0.00 0.05580 22400.00 10800.00 0.00 0.05584 22000.00 11000.00 0.00 0.05634 22200.00 11000.00 0.00 0.05661 22000.00 11200.00 0.00 0.05694 22200.00 11200.00 0.00 0.05713 22000.00 11400.00 0.00 0.05759 22200.00 11400.00 0.00 0.05774 21800.00 11600.00 0.00 0.05795 22000.00 11600.00 0.00 0.05845 22200.00 11600.00 0.00 0.05857 21800.00 11800.00 0.00 0.05883 22000.00 11800.00 0.00 0.05931 22200.00 11800.00 0.00 0.05935 21800.00 12000.00 0.00 0.05972 22000.00 12000.00 0.00 0.06015 21800.00 12200.00 0.00 0.06064 22000.00 12200.00 0.00 0.06106 22200.00 12200.00 0.00 0.06106 21800.00 12400.00 0.00 0.06147 22000.00 12400.00 0.00 0.06191 22200.00 12400.00 0.00 0.06191 21800.00 12600.00 0.00 0.06228 22000.00 12600.00 0.00 0.06270 21800.00 12800.00 0.00 0.06318 22000.00 12800.00 0.00 0.06356 21800.00 13000.00 0.00 0.06403 22000.00 13000.00 0.00 0.06438 21800.00 13200.00 0.00 0.06499 22000.00 13200.00 0.00 0.06533 21800.00 13400.00 0.00 0.06599 22000.00 13400.00 0.00 0.06637 21800.00 13600.00 0.00 0.06708 22000.00 13600.00 0.00 0.06749 21800.00 13800.00 0.00 0.06830 22000.00 13800.00 0.00 0.06869 21800.00 14000.00 0.00 0.06943 22000.00 14000.00 0.00 0.06976 21800.00 14200.00 0.00 0.07046 22000.00 14200.00 0.00 0.07075 21800.00 14400.00 0.00 0.07164 22000.00 14400.00 0.00 0.07191 21800.00 14600.00 0.00 0.07286 22000.00 14600.00 0.00 0.07312 21800.00 14800.00 0.00 0.07394 22000.00 14800.00 0.00 0.07419 21600.00 15000.00 0.00 0.07426 21800.00 15000.00 0.00 0.07525 22000.00 15000.00 0.00 0.07546 21600.00 15200.00 0.00 0.07559 21800.00 15200.00 0.00 0.07649 22000.00 15200.00 0.00 0.07666 21600.00 15400.00 0.00 0.07675 21800.00 15400.00 0.00 0.07761 22000.00 15400.00 0.00 0.07784 21600.00 15600.00 0.00 0.07804 21800.00 15600.00 0.00 0.07894 22000.00 15600.00 0.00 0.07925 21600.00 15800.00 0.00 0.07939 21800.00 15800.00 0.00 0.08036 22000.00 15800.00 0.00 0.08068 21600.00 16000.00 0.00 0.08084 21800.00 16000.00 0.00 0.08182 22000.00 16000.00 0.00 0.08207 21600.00 16200.00 0.00 0.08219 21800.00 16200.00 0.00 0.08316 22000.00 16200.00 0.00 0.08336 21600.00 16400.00 0.00 0.08371 21800.00 16400.00 0.00 0.08469 22000.00 16400.00 0.00 0.08485 21600.00 16600.00 0.00 0.08525 21800.00 16600.00 0.00 0.08622 22000.00 16600.00 0.00 0.08635 21600.00 16800.00 0.00 0.08683 21800.00 16800.00 0.00 0.08775 22000.00 16800.00 0.00 0.08783 21600.00 17000.00 0.00 0.08842 21800.00 17000.00 0.00 0.08930 22000.00 17000.00 0.00 0.08936 21600.00 17200.00 0.00 0.08994 21800.00 17200.00 0.00 0.09079 22000.00 17200.00 0.00 0.09086 21600.00 17400.00 0.00 0.09150 21800.00 17400.00 0.00 0.09235 22000.00 17400.00 0.00 0.09239 21600.00 17600.00 0.00 0.09296 21800.00 17600.00 0.00 0.09379 22000.00 17600.00 0.00 0.09381 21600.00 17800.00 0.00 0.09457 21800.00 17800.00 0.00 0.09540 21600.00 18000.00 0.00 0.09620 21800.00 18000.00 0.00 0.09703 21600.00 18200.00 0.00 0.09793 21800.00 18200.00 0.00 0.09874 21600.00 18400.00 0.00 0.09970 21800.00 18400.00 0.00 0.10046 21600.00 18600.00 0.00 0.10140 21800.00 18600.00 0.00 0.10209 21600.00 18800.00 0.00 0.10315 21800.00 18800.00 0.00 0.10375 21600.00 19000.00 0.00 0.10479 21800.00 19000.00 0.00 0.10529 21600.00 19200.00 0.00 0.10667 21800.00 19200.00 0.00 0.10707 21600.00 19400.00 0.00 0.10862 21800.00 19400.00 0.00 0.10895 21600.00 19600.00 0.00 0.11054 21800.00 19600.00 0.00 0.11081 21600.00 19800.00 0.00 0.11229 21800.00 19800.00 0.00 0.11246 21400.00 20000.00 0.00 0.11255 21600.00 20000.00 0.00 0.11407 21800.00 20000.00 0.00 0.11412 21400.00 20200.00 0.00 0.11434 21600.00 20200.00 0.00 0.11580 21400.00 20400.00 0.00 0.11600 21600.00 20400.00 0.00 0.11740 21400.00 20600.00 0.00 0.11789 21600.00 20600.00 0.00 0.11917 21400.00 20800.00 0.00 0.11951 21600.00 20800.00 0.00 0.12061 21400.00 21000.00 0.00 0.12121 21600.00 21000.00 0.00 0.12214 21400.00 21200.00 0.00 0.12251 21600.00 21200.00 0.00 0.12332 21400.00 21400.00 0.00 0.12368 21600.00 21400.00 0.00 0.12433 21400.00 21600.00 0.00 0.12468 21600.00 21600.00 0.00 0.12494 21400.00 21600.00 20.00 0.12494 21400.00 21800.00 20.00 0.12501 21400.00 21800.00 25.00 0.12506 21400.00 21800.00 30.00 0.12506 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21470.58 21665.05 27.98 0.12526 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/022.ctf NX, NY, NZ: 512 512 1 MODE : real