CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/020.mrc Output diagnostic file name Micrographs_0601/020.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/020.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2590.000 7738.000 Mean, RMS : 4480.745 219.8328 TITLE 1: emi2mrc: 020.emi Sun Jan 05 13:37:37 2014 TITLE 2: kV 300, Mag 78000x, Def 27890, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X 337.026, Y 286.326, Z -105.479, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00792 10600.00 10000.00 0.00 -0.00787 10800.00 10000.00 0.00 -0.00767 11000.00 10000.00 0.00 -0.00753 11200.00 10000.00 0.00 -0.00743 11400.00 10000.00 0.00 -0.00726 11600.00 10000.00 0.00 -0.00719 12000.00 10000.00 0.00 -0.00692 12200.00 10000.00 0.00 -0.00654 12400.00 10000.00 0.00 -0.00631 12600.00 10000.00 0.00 -0.00607 12800.00 10000.00 0.00 -0.00577 13000.00 10000.00 0.00 -0.00538 13200.00 10000.00 0.00 -0.00507 13400.00 10000.00 0.00 -0.00495 13600.00 10000.00 0.00 -0.00464 13800.00 10000.00 0.00 -0.00411 14000.00 10000.00 0.00 -0.00365 14200.00 10000.00 0.00 -0.00333 14400.00 10000.00 0.00 -0.00299 14600.00 10000.00 0.00 -0.00253 14800.00 10000.00 0.00 -0.00195 15000.00 10000.00 0.00 -0.00137 15200.00 10000.00 0.00 -0.00071 15400.00 10000.00 0.00 0.00013 15600.00 10000.00 0.00 0.00094 15800.00 10000.00 0.00 0.00163 16000.00 10000.00 0.00 0.00229 16200.00 10000.00 0.00 0.00292 16400.00 10000.00 0.00 0.00360 16600.00 10000.00 0.00 0.00444 16800.00 10000.00 0.00 0.00543 17000.00 10000.00 0.00 0.00642 17200.00 10000.00 0.00 0.00742 17400.00 10000.00 0.00 0.00854 17600.00 10000.00 0.00 0.00962 17800.00 10000.00 0.00 0.01065 18000.00 10000.00 0.00 0.01180 18200.00 10000.00 0.00 0.01291 18400.00 10000.00 0.00 0.01396 18600.00 10000.00 0.00 0.01510 18800.00 10000.00 0.00 0.01613 19000.00 10000.00 0.00 0.01708 19200.00 10000.00 0.00 0.01811 19400.00 10000.00 0.00 0.01917 19600.00 10000.00 0.00 0.02023 19800.00 10000.00 0.00 0.02123 20000.00 10000.00 0.00 0.02231 20200.00 10000.00 0.00 0.02358 20400.00 10000.00 0.00 0.02490 20600.00 10000.00 0.00 0.02618 20800.00 10000.00 0.00 0.02738 21000.00 10000.00 0.00 0.02867 21200.00 10000.00 0.00 0.02991 21400.00 10000.00 0.00 0.03110 21600.00 10000.00 0.00 0.03247 21800.00 10000.00 0.00 0.03386 22000.00 10000.00 0.00 0.03532 22200.00 10000.00 0.00 0.03695 22400.00 10000.00 0.00 0.03869 22600.00 10000.00 0.00 0.04045 22800.00 10000.00 0.00 0.04221 23000.00 10000.00 0.00 0.04407 23200.00 10000.00 0.00 0.04588 23400.00 10000.00 0.00 0.04735 23600.00 10000.00 0.00 0.04833 23800.00 10000.00 0.00 0.04890 24000.00 10000.00 0.00 0.04910 23800.00 10200.00 0.00 0.04930 24000.00 10200.00 0.00 0.04947 23800.00 10400.00 0.00 0.04962 24000.00 10400.00 0.00 0.04975 23800.00 10600.00 0.00 0.05000 24000.00 10600.00 0.00 0.05008 23800.00 10800.00 0.00 0.05044 24000.00 10800.00 0.00 0.05049 23800.00 11000.00 0.00 0.05094 24000.00 11000.00 0.00 0.05096 23800.00 11200.00 0.00 0.05130 24000.00 11200.00 0.00 0.05131 23600.00 11400.00 0.00 0.05131 23800.00 11400.00 0.00 0.05173 24000.00 11400.00 0.00 0.05176 23600.00 11600.00 0.00 0.05187 23800.00 11600.00 0.00 0.05230 24000.00 11600.00 0.00 0.05233 23600.00 11800.00 0.00 0.05256 23800.00 11800.00 0.00 0.05297 23600.00 12000.00 0.00 0.05335 23800.00 12000.00 0.00 0.05370 23600.00 12200.00 0.00 0.05404 23800.00 12200.00 0.00 0.05438 23600.00 12400.00 0.00 0.05473 23800.00 12400.00 0.00 0.05509 23600.00 12600.00 0.00 0.05546 23800.00 12600.00 0.00 0.05579 23600.00 12800.00 0.00 0.05617 23800.00 12800.00 0.00 0.05642 23600.00 13000.00 0.00 0.05681 23800.00 13000.00 0.00 0.05704 23600.00 13200.00 0.00 0.05744 23800.00 13200.00 0.00 0.05769 23600.00 13400.00 0.00 0.05811 23800.00 13400.00 0.00 0.05838 23600.00 13600.00 0.00 0.05890 23800.00 13600.00 0.00 0.05916 24000.00 13600.00 0.00 0.05917 23600.00 13800.00 0.00 0.05967 23800.00 13800.00 0.00 0.05996 24000.00 13800.00 0.00 0.05999 23600.00 14000.00 0.00 0.06041 23800.00 14000.00 0.00 0.06070 24000.00 14000.00 0.00 0.06078 23600.00 14200.00 0.00 0.06117 23800.00 14200.00 0.00 0.06148 24000.00 14200.00 0.00 0.06159 23600.00 14400.00 0.00 0.06194 23800.00 14400.00 0.00 0.06228 24000.00 14400.00 0.00 0.06241 23600.00 14600.00 0.00 0.06278 23800.00 14600.00 0.00 0.06314 24000.00 14600.00 0.00 0.06328 23600.00 14800.00 0.00 0.06364 23800.00 14800.00 0.00 0.06402 24000.00 14800.00 0.00 0.06413 23600.00 15000.00 0.00 0.06453 23800.00 15000.00 0.00 0.06491 24000.00 15000.00 0.00 0.06496 23600.00 15200.00 0.00 0.06539 23800.00 15200.00 0.00 0.06575 24000.00 15200.00 0.00 0.06578 23600.00 15400.00 0.00 0.06629 23800.00 15400.00 0.00 0.06666 24000.00 15400.00 0.00 0.06666 23600.00 15600.00 0.00 0.06723 23800.00 15600.00 0.00 0.06761 23600.00 15800.00 0.00 0.06821 23800.00 15800.00 0.00 0.06857 23600.00 16000.00 0.00 0.06932 23800.00 16000.00 0.00 0.06965 23600.00 16200.00 0.00 0.07048 23800.00 16200.00 0.00 0.07075 23400.00 16400.00 0.00 0.07079 23600.00 16400.00 0.00 0.07163 23800.00 16400.00 0.00 0.07185 23600.00 16600.00 0.00 0.07266 23800.00 16600.00 0.00 0.07288 23400.00 16800.00 0.00 0.07297 23600.00 16800.00 0.00 0.07379 23800.00 16800.00 0.00 0.07400 23400.00 17000.00 0.00 0.07419 23600.00 17000.00 0.00 0.07498 23800.00 17000.00 0.00 0.07519 23400.00 17200.00 0.00 0.07534 23600.00 17200.00 0.00 0.07612 23800.00 17200.00 0.00 0.07635 23400.00 17400.00 0.00 0.07642 23600.00 17400.00 0.00 0.07723 23800.00 17400.00 0.00 0.07746 23400.00 17600.00 0.00 0.07747 23600.00 17600.00 0.00 0.07830 23800.00 17600.00 0.00 0.07851 23400.00 17800.00 0.00 0.07876 23600.00 17800.00 0.00 0.07958 23800.00 17800.00 0.00 0.07977 23400.00 18000.00 0.00 0.08005 23600.00 18000.00 0.00 0.08086 23800.00 18000.00 0.00 0.08105 23400.00 18200.00 0.00 0.08136 23600.00 18200.00 0.00 0.08216 23800.00 18200.00 0.00 0.08236 23400.00 18400.00 0.00 0.08266 23600.00 18400.00 0.00 0.08348 23800.00 18400.00 0.00 0.08367 23400.00 18600.00 0.00 0.08407 23600.00 18600.00 0.00 0.08490 23800.00 18600.00 0.00 0.08505 23400.00 18800.00 0.00 0.08556 23600.00 18800.00 0.00 0.08635 23800.00 18800.00 0.00 0.08643 23400.00 19000.00 0.00 0.08684 23600.00 19000.00 0.00 0.08754 23400.00 19200.00 0.00 0.08811 23600.00 19200.00 0.00 0.08873 23400.00 19400.00 0.00 0.08919 23600.00 19400.00 0.00 0.08977 23400.00 19600.00 0.00 0.09031 23600.00 19600.00 0.00 0.09088 23400.00 19800.00 0.00 0.09156 23600.00 19800.00 0.00 0.09210 23400.00 20000.00 0.00 0.09275 23600.00 20000.00 0.00 0.09322 23400.00 20200.00 0.00 0.09389 23600.00 20200.00 0.00 0.09432 23400.00 20400.00 0.00 0.09498 23600.00 20400.00 0.00 0.09537 23400.00 20600.00 0.00 0.09611 23600.00 20600.00 0.00 0.09646 23400.00 20800.00 0.00 0.09722 23600.00 20800.00 0.00 0.09756 23400.00 21000.00 0.00 0.09862 23600.00 21000.00 0.00 0.09893 23400.00 21200.00 0.00 0.09994 23600.00 21200.00 0.00 0.10017 23400.00 21400.00 0.00 0.10113 23600.00 21400.00 0.00 0.10126 23400.00 21600.00 0.00 0.10228 23600.00 21600.00 0.00 0.10230 23400.00 21800.00 0.00 0.10340 23200.00 22000.00 0.00 0.10347 23400.00 22000.00 0.00 0.10459 23200.00 22200.00 0.00 0.10460 23400.00 22200.00 0.00 0.10566 23200.00 22400.00 0.00 0.10593 23400.00 22400.00 0.00 0.10688 23200.00 22600.00 0.00 0.10713 23400.00 22600.00 0.00 0.10797 23200.00 22800.00 0.00 0.10840 23400.00 22800.00 0.00 0.10911 23200.00 23000.00 0.00 0.10944 23400.00 23000.00 0.00 0.11007 23200.00 23200.00 0.00 0.11036 23400.00 23200.00 0.00 0.11089 23200.00 23400.00 0.00 0.11108 23400.00 23400.00 0.00 0.11129 23200.00 23400.00 15.00 0.11135 23200.00 23400.00 20.00 0.11143 23200.00 23400.00 25.00 0.11149 23200.00 23400.00 30.00 0.11154 23200.00 23400.00 35.00 0.11158 23200.00 23400.00 40.00 0.11159 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23232.02 23465.91 35.99 0.11173 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/020.ctf NX, NY, NZ: 512 512 1 MODE : real