CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/012.mrc Output diagnostic file name Micrographs_0601/012.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/012.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2512.000 8712.000 Mean, RMS : 4496.017 216.7040 TITLE 1: emi2mrc: 012.emi Sun Jan 05 13:28:07 2014 TITLE 2: kV 300, Mag 78000x, Def 27240, Spot 4, Int 55.10%, Obj 88.04%, Dif 73.67% TITLE 3: X 335.973, Y 275.907, Z -105.996, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02861 22600.00 10000.00 0.00 -0.02818 22800.00 10000.00 0.00 -0.02749 23000.00 10000.00 0.00 -0.02678 23200.00 10000.00 0.00 -0.02600 23400.00 10000.00 0.00 -0.02528 23600.00 10000.00 0.00 -0.02453 23800.00 10000.00 0.00 -0.02391 24000.00 10000.00 0.00 -0.02337 24200.00 10000.00 0.00 -0.02272 24400.00 10000.00 0.00 -0.02214 24600.00 10000.00 0.00 -0.02162 24800.00 10000.00 0.00 -0.02100 25000.00 10000.00 0.00 -0.02041 25200.00 10000.00 0.00 -0.01990 25400.00 10000.00 0.00 -0.01931 25600.00 10000.00 0.00 -0.01847 25800.00 10000.00 0.00 -0.01751 26000.00 10000.00 0.00 -0.01655 26200.00 10000.00 0.00 -0.01548 26400.00 10000.00 0.00 -0.01439 26600.00 10000.00 0.00 -0.01339 26800.00 10000.00 0.00 -0.01237 27000.00 10000.00 0.00 -0.01116 27200.00 10000.00 0.00 -0.00980 27400.00 10000.00 0.00 -0.00861 27600.00 10000.00 0.00 -0.00758 27800.00 10000.00 0.00 -0.00650 28000.00 10000.00 0.00 -0.00528 28200.00 10000.00 0.00 -0.00404 28400.00 10000.00 0.00 -0.00284 28600.00 10000.00 0.00 -0.00147 28800.00 10000.00 0.00 0.00015 29000.00 10000.00 0.00 0.00181 29200.00 10000.00 0.00 0.00340 29400.00 10000.00 0.00 0.00494 29600.00 10000.00 0.00 0.00646 29800.00 10000.00 0.00 0.00811 30000.00 10000.00 0.00 0.00985 30200.00 10000.00 0.00 0.01153 30400.00 10000.00 0.00 0.01289 30600.00 10000.00 0.00 0.01356 30800.00 10000.00 0.00 0.01386 31000.00 10000.00 0.00 0.01396 31000.00 10200.00 0.00 0.01401 30800.00 10400.00 0.00 0.01410 31000.00 10400.00 0.00 0.01419 30800.00 10600.00 0.00 0.01439 31000.00 10600.00 0.00 0.01447 30800.00 10800.00 0.00 0.01473 31000.00 10800.00 0.00 0.01477 30800.00 11000.00 0.00 0.01499 31000.00 11000.00 0.00 0.01499 30800.00 11200.00 0.00 0.01515 30800.00 11400.00 0.00 0.01530 30800.00 11600.00 0.00 0.01540 31000.00 11600.00 0.00 0.01541 30800.00 11800.00 0.00 0.01553 31000.00 11800.00 0.00 0.01556 30800.00 12000.00 0.00 0.01562 31000.00 12000.00 0.00 0.01563 30800.00 12200.00 0.00 0.01571 30800.00 12400.00 0.00 0.01587 30800.00 12600.00 0.00 0.01607 30800.00 12800.00 0.00 0.01623 30800.00 13200.00 0.00 0.01629 30800.00 13400.00 0.00 0.01645 30800.00 13600.00 0.00 0.01658 30600.00 13800.00 0.00 0.01666 30800.00 13800.00 0.00 0.01676 30600.00 14000.00 0.00 0.01679 30800.00 14000.00 0.00 0.01681 30600.00 14200.00 0.00 0.01684 30600.00 14400.00 0.00 0.01692 30600.00 14600.00 0.00 0.01711 30600.00 14800.00 0.00 0.01727 30600.00 15000.00 0.00 0.01752 30800.00 15000.00 0.00 0.01753 30600.00 15200.00 0.00 0.01793 30800.00 15200.00 0.00 0.01799 30600.00 15400.00 0.00 0.01823 30800.00 15400.00 0.00 0.01832 30600.00 15600.00 0.00 0.01863 30800.00 15600.00 0.00 0.01872 30600.00 15800.00 0.00 0.01907 30800.00 15800.00 0.00 0.01915 30600.00 16000.00 0.00 0.01933 30800.00 16000.00 0.00 0.01941 30600.00 16200.00 0.00 0.01964 30800.00 16200.00 0.00 0.01973 30600.00 16400.00 0.00 0.02001 30800.00 16400.00 0.00 0.02005 30600.00 16600.00 0.00 0.02044 30800.00 16600.00 0.00 0.02045 30400.00 16800.00 0.00 0.02064 30600.00 16800.00 0.00 0.02109 30800.00 16800.00 0.00 0.02111 30400.00 17000.00 0.00 0.02134 30600.00 17000.00 0.00 0.02178 30800.00 17000.00 0.00 0.02187 30400.00 17200.00 0.00 0.02201 30600.00 17200.00 0.00 0.02248 30800.00 17200.00 0.00 0.02264 30600.00 17400.00 0.00 0.02316 30800.00 17400.00 0.00 0.02337 30600.00 17600.00 0.00 0.02380 30800.00 17600.00 0.00 0.02401 30600.00 17800.00 0.00 0.02458 30800.00 17800.00 0.00 0.02472 30600.00 18000.00 0.00 0.02525 30800.00 18000.00 0.00 0.02534 30600.00 18200.00 0.00 0.02587 30800.00 18200.00 0.00 0.02593 30400.00 18400.00 0.00 0.02597 30600.00 18400.00 0.00 0.02652 30800.00 18400.00 0.00 0.02655 30600.00 18600.00 0.00 0.02705 30800.00 18600.00 0.00 0.02709 30600.00 18800.00 0.00 0.02766 30800.00 18800.00 0.00 0.02774 30600.00 19000.00 0.00 0.02835 30800.00 19000.00 0.00 0.02842 30600.00 19200.00 0.00 0.02894 30800.00 19200.00 0.00 0.02897 30600.00 19400.00 0.00 0.02959 30800.00 19400.00 0.00 0.02962 30400.00 19600.00 0.00 0.02982 30600.00 19600.00 0.00 0.03044 30800.00 19600.00 0.00 0.03048 30400.00 19800.00 0.00 0.03069 30600.00 19800.00 0.00 0.03131 30800.00 19800.00 0.00 0.03136 30400.00 20000.00 0.00 0.03138 30600.00 20000.00 0.00 0.03203 30800.00 20000.00 0.00 0.03206 30600.00 20200.00 0.00 0.03270 30400.00 20400.00 0.00 0.03273 30600.00 20400.00 0.00 0.03336 30400.00 20600.00 0.00 0.03336 30600.00 20600.00 0.00 0.03399 30400.00 20800.00 0.00 0.03415 30600.00 20800.00 0.00 0.03478 30400.00 21000.00 0.00 0.03516 30600.00 21000.00 0.00 0.03576 30400.00 21200.00 0.00 0.03608 30600.00 21200.00 0.00 0.03660 30400.00 21400.00 0.00 0.03684 30600.00 21400.00 0.00 0.03729 30400.00 21600.00 0.00 0.03763 30600.00 21600.00 0.00 0.03804 30400.00 21800.00 0.00 0.03840 30600.00 21800.00 0.00 0.03879 30400.00 22000.00 0.00 0.03916 30600.00 22000.00 0.00 0.03960 30400.00 22200.00 0.00 0.04003 30600.00 22200.00 0.00 0.04049 30400.00 22400.00 0.00 0.04100 30600.00 22400.00 0.00 0.04141 30400.00 22600.00 0.00 0.04202 30600.00 22600.00 0.00 0.04238 30400.00 22800.00 0.00 0.04293 30600.00 22800.00 0.00 0.04327 30400.00 23000.00 0.00 0.04382 30600.00 23000.00 0.00 0.04416 30400.00 23200.00 0.00 0.04485 30600.00 23200.00 0.00 0.04517 30400.00 23400.00 0.00 0.04580 30600.00 23400.00 0.00 0.04608 30400.00 23600.00 0.00 0.04666 30600.00 23600.00 0.00 0.04691 30400.00 23800.00 0.00 0.04764 30600.00 23800.00 0.00 0.04785 30400.00 24000.00 0.00 0.04862 30600.00 24000.00 0.00 0.04879 30400.00 24200.00 0.00 0.04952 30600.00 24200.00 0.00 0.04963 30400.00 24400.00 0.00 0.05034 30600.00 24400.00 0.00 0.05038 30400.00 24600.00 0.00 0.05106 30600.00 24600.00 0.00 0.05106 30400.00 24800.00 0.00 0.05173 30600.00 24800.00 0.00 0.05174 30400.00 25000.00 0.00 0.05252 30400.00 25200.00 0.00 0.05324 30400.00 25400.00 0.00 0.05388 30400.00 25600.00 0.00 0.05465 30400.00 25800.00 0.00 0.05549 30400.00 26000.00 0.00 0.05646 30400.00 26200.00 0.00 0.05742 30400.00 26400.00 0.00 0.05826 30400.00 26600.00 0.00 0.05904 30400.00 26800.00 0.00 0.05984 30400.00 27000.00 0.00 0.06063 30400.00 27200.00 0.00 0.06122 30400.00 27400.00 0.00 0.06171 30400.00 27600.00 0.00 0.06229 30200.00 27800.00 0.00 0.06244 30400.00 27800.00 0.00 0.06304 30200.00 28000.00 0.00 0.06309 30400.00 28000.00 0.00 0.06366 30200.00 28200.00 0.00 0.06373 30400.00 28200.00 0.00 0.06431 30200.00 28400.00 0.00 0.06451 30400.00 28400.00 0.00 0.06512 30200.00 28600.00 0.00 0.06522 30400.00 28600.00 0.00 0.06578 30200.00 28800.00 0.00 0.06594 30400.00 28800.00 0.00 0.06644 30200.00 29000.00 0.00 0.06665 30400.00 29000.00 0.00 0.06710 30200.00 29200.00 0.00 0.06746 30400.00 29200.00 0.00 0.06782 30200.00 29400.00 0.00 0.06814 30400.00 29400.00 0.00 0.06839 30200.00 29600.00 0.00 0.06881 30400.00 29600.00 0.00 0.06899 30200.00 29800.00 0.00 0.06957 30400.00 29800.00 0.00 0.06962 30200.00 30000.00 0.00 0.07014 30200.00 30200.00 0.00 0.07064 30200.00 30400.00 0.00 0.07066 30200.00 30400.00 5.00 0.07072 30200.00 30400.00 10.00 0.07078 30200.00 30400.00 15.00 0.07084 30200.00 30400.00 20.00 0.07090 30200.00 30400.00 25.00 0.07095 30200.00 30400.00 30.00 0.07098 30200.00 30400.00 35.00 0.07101 30200.00 30400.00 40.00 0.07101 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 30165.27 30383.44 40.39 0.07103 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/012.ctf NX, NY, NZ: 512 512 1 MODE : real