CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/008.mrc Output diagnostic file name Micrographs_0601/008.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/008.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2386.000 8416.000 Mean, RMS : 4534.079 220.3930 TITLE 1: emi2mrc: 008.emi Sun Jan 05 12:35:25 2014 TITLE 2: kV 300, Mag 78000x, Def 29040, Spot 4, Int 55.10%, Obj 88.04%, Dif 73.67% TITLE 3: X 346.896, Y 276.972, Z -105.996, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.03677 25200.00 10000.00 0.00 -0.03663 25400.00 10000.00 0.00 -0.03618 25600.00 10000.00 0.00 -0.03563 25800.00 10000.00 0.00 -0.03503 26000.00 10000.00 0.00 -0.03451 26200.00 10000.00 0.00 -0.03422 26400.00 10000.00 0.00 -0.03395 26600.00 10000.00 0.00 -0.03364 26800.00 10000.00 0.00 -0.03340 27000.00 10000.00 0.00 -0.03313 27200.00 10000.00 0.00 -0.03264 27400.00 10000.00 0.00 -0.03216 27600.00 10000.00 0.00 -0.03165 27800.00 10000.00 0.00 -0.03098 28000.00 10000.00 0.00 -0.03023 28200.00 10000.00 0.00 -0.02947 28400.00 10000.00 0.00 -0.02881 28600.00 10000.00 0.00 -0.02806 28800.00 10000.00 0.00 -0.02723 29000.00 10000.00 0.00 -0.02640 29200.00 10000.00 0.00 -0.02557 29400.00 10000.00 0.00 -0.02491 29600.00 10000.00 0.00 -0.02427 29800.00 10000.00 0.00 -0.02361 30000.00 10000.00 0.00 -0.02293 30200.00 10000.00 0.00 -0.02201 30400.00 10000.00 0.00 -0.02106 30600.00 10000.00 0.00 -0.02036 30800.00 10000.00 0.00 -0.01953 31000.00 10000.00 0.00 -0.01849 31200.00 10000.00 0.00 -0.01758 31400.00 10000.00 0.00 -0.01657 31600.00 10000.00 0.00 -0.01533 31800.00 10000.00 0.00 -0.01379 32000.00 10000.00 0.00 -0.01209 32200.00 10000.00 0.00 -0.01050 32400.00 10000.00 0.00 -0.00892 32600.00 10000.00 0.00 -0.00777 32800.00 10000.00 0.00 -0.00732 33000.00 10000.00 0.00 -0.00706 33200.00 10000.00 0.00 -0.00679 33400.00 10000.00 0.00 -0.00677 33000.00 14200.00 0.00 -0.00665 33200.00 14200.00 0.00 -0.00651 33000.00 14400.00 0.00 -0.00633 33200.00 14400.00 0.00 -0.00617 33000.00 14600.00 0.00 -0.00600 33200.00 14600.00 0.00 -0.00583 33000.00 14800.00 0.00 -0.00582 33200.00 14800.00 0.00 -0.00564 33000.00 15000.00 0.00 -0.00559 33200.00 15000.00 0.00 -0.00541 33000.00 15200.00 0.00 -0.00534 33200.00 15200.00 0.00 -0.00520 33000.00 15400.00 0.00 -0.00511 33200.00 15400.00 0.00 -0.00498 33000.00 15600.00 0.00 -0.00486 33200.00 15600.00 0.00 -0.00470 33000.00 15800.00 0.00 -0.00458 33200.00 15800.00 0.00 -0.00443 33000.00 16000.00 0.00 -0.00417 33200.00 16000.00 0.00 -0.00405 32800.00 16200.00 0.00 -0.00404 33000.00 16200.00 0.00 -0.00370 33200.00 16200.00 0.00 -0.00357 32800.00 16400.00 0.00 -0.00346 33000.00 16400.00 0.00 -0.00312 33200.00 16400.00 0.00 -0.00295 32800.00 16600.00 0.00 -0.00289 33000.00 16600.00 0.00 -0.00253 33200.00 16600.00 0.00 -0.00231 33000.00 16800.00 0.00 -0.00201 33200.00 16800.00 0.00 -0.00177 33000.00 17000.00 0.00 -0.00151 33200.00 17000.00 0.00 -0.00130 33000.00 17200.00 0.00 -0.00106 33200.00 17200.00 0.00 -0.00095 33000.00 17400.00 0.00 -0.00068 33200.00 17400.00 0.00 -0.00066 33000.00 17600.00 0.00 -0.00033 32800.00 17800.00 0.00 -0.00012 33000.00 17800.00 0.00 0.00019 32800.00 18000.00 0.00 0.00048 33000.00 18000.00 0.00 0.00075 32800.00 18200.00 0.00 0.00088 33000.00 18200.00 0.00 0.00115 32800.00 18400.00 0.00 0.00127 33000.00 18400.00 0.00 0.00155 32800.00 18600.00 0.00 0.00167 33000.00 18600.00 0.00 0.00191 32800.00 18800.00 0.00 0.00201 33000.00 18800.00 0.00 0.00220 32800.00 19000.00 0.00 0.00231 33000.00 19000.00 0.00 0.00249 32800.00 19200.00 0.00 0.00259 33000.00 19200.00 0.00 0.00279 32800.00 19400.00 0.00 0.00293 33000.00 19400.00 0.00 0.00313 32800.00 19600.00 0.00 0.00338 33000.00 19600.00 0.00 0.00357 32800.00 19800.00 0.00 0.00387 33000.00 19800.00 0.00 0.00405 32800.00 20000.00 0.00 0.00440 33000.00 20000.00 0.00 0.00454 32800.00 20200.00 0.00 0.00495 33000.00 20200.00 0.00 0.00507 32800.00 20400.00 0.00 0.00551 33000.00 20400.00 0.00 0.00566 33200.00 20400.00 0.00 0.00567 32600.00 20600.00 0.00 0.00571 32800.00 20600.00 0.00 0.00617 33000.00 20600.00 0.00 0.00639 33200.00 20600.00 0.00 0.00644 32800.00 20800.00 0.00 0.00691 33000.00 20800.00 0.00 0.00718 33200.00 20800.00 0.00 0.00725 32800.00 21000.00 0.00 0.00762 33000.00 21000.00 0.00 0.00794 33200.00 21000.00 0.00 0.00801 32800.00 21200.00 0.00 0.00843 33000.00 21200.00 0.00 0.00878 33200.00 21200.00 0.00 0.00883 32800.00 21400.00 0.00 0.00922 33000.00 21400.00 0.00 0.00957 33200.00 21400.00 0.00 0.00959 32800.00 21600.00 0.00 0.00991 33000.00 21600.00 0.00 0.01024 32800.00 21800.00 0.00 0.01073 33000.00 21800.00 0.00 0.01103 32800.00 22000.00 0.00 0.01158 33000.00 22000.00 0.00 0.01183 32800.00 22200.00 0.00 0.01236 33000.00 22200.00 0.00 0.01252 32800.00 22400.00 0.00 0.01301 33000.00 22400.00 0.00 0.01310 32800.00 22600.00 0.00 0.01360 33000.00 22600.00 0.00 0.01371 32600.00 22800.00 0.00 0.01373 32800.00 22800.00 0.00 0.01437 33000.00 22800.00 0.00 0.01452 32600.00 23000.00 0.00 0.01456 32800.00 23000.00 0.00 0.01522 33000.00 23000.00 0.00 0.01538 32600.00 23200.00 0.00 0.01544 32800.00 23200.00 0.00 0.01610 33000.00 23200.00 0.00 0.01626 32600.00 23400.00 0.00 0.01626 32800.00 23400.00 0.00 0.01694 33000.00 23400.00 0.00 0.01709 32600.00 23600.00 0.00 0.01714 32800.00 23600.00 0.00 0.01784 33000.00 23600.00 0.00 0.01798 32600.00 23800.00 0.00 0.01822 32800.00 23800.00 0.00 0.01890 33000.00 23800.00 0.00 0.01898 32600.00 24000.00 0.00 0.01916 32800.00 24000.00 0.00 0.01978 33000.00 24000.00 0.00 0.01982 32600.00 24200.00 0.00 0.01998 32800.00 24200.00 0.00 0.02061 33000.00 24200.00 0.00 0.02066 32600.00 24400.00 0.00 0.02082 32800.00 24400.00 0.00 0.02150 33000.00 24400.00 0.00 0.02154 32600.00 24600.00 0.00 0.02183 32800.00 24600.00 0.00 0.02247 32600.00 24800.00 0.00 0.02287 32800.00 24800.00 0.00 0.02336 32600.00 25000.00 0.00 0.02378 32800.00 25000.00 0.00 0.02415 32600.00 25200.00 0.00 0.02465 32800.00 25200.00 0.00 0.02500 32600.00 25400.00 0.00 0.02548 32800.00 25400.00 0.00 0.02588 32600.00 25600.00 0.00 0.02644 32800.00 25600.00 0.00 0.02687 32600.00 25800.00 0.00 0.02726 32800.00 25800.00 0.00 0.02770 32600.00 26000.00 0.00 0.02809 32800.00 26000.00 0.00 0.02854 32600.00 26200.00 0.00 0.02910 32800.00 26200.00 0.00 0.02956 32600.00 26400.00 0.00 0.03008 32800.00 26400.00 0.00 0.03055 32600.00 26600.00 0.00 0.03107 32800.00 26600.00 0.00 0.03154 32600.00 26800.00 0.00 0.03200 32800.00 26800.00 0.00 0.03245 32600.00 27000.00 0.00 0.03319 32800.00 27000.00 0.00 0.03358 32600.00 27200.00 0.00 0.03441 32800.00 27200.00 0.00 0.03478 32600.00 27400.00 0.00 0.03552 32800.00 27400.00 0.00 0.03594 32600.00 27600.00 0.00 0.03688 32800.00 27600.00 0.00 0.03736 32600.00 27800.00 0.00 0.03824 32800.00 27800.00 0.00 0.03868 32600.00 28000.00 0.00 0.03945 32800.00 28000.00 0.00 0.03987 32600.00 28200.00 0.00 0.04065 32800.00 28200.00 0.00 0.04106 32600.00 28400.00 0.00 0.04194 32800.00 28400.00 0.00 0.04232 32600.00 28600.00 0.00 0.04319 32800.00 28600.00 0.00 0.04348 32600.00 28800.00 0.00 0.04424 32800.00 28800.00 0.00 0.04447 32600.00 29000.00 0.00 0.04544 32800.00 29000.00 0.00 0.04565 32600.00 29200.00 0.00 0.04674 32800.00 29200.00 0.00 0.04692 32600.00 29400.00 0.00 0.04802 32800.00 29400.00 0.00 0.04814 32400.00 29600.00 0.00 0.04831 32600.00 29600.00 0.00 0.04949 32800.00 29600.00 0.00 0.04955 32400.00 29800.00 0.00 0.04997 32600.00 29800.00 0.00 0.05110 32400.00 30000.00 0.00 0.05181 32600.00 30000.00 0.00 0.05289 32400.00 30200.00 0.00 0.05373 32600.00 30200.00 0.00 0.05476 32400.00 30400.00 0.00 0.05542 32600.00 30400.00 0.00 0.05636 32400.00 30600.00 0.00 0.05714 32600.00 30600.00 0.00 0.05801 32400.00 30800.00 0.00 0.05892 32600.00 30800.00 0.00 0.05969 32400.00 31000.00 0.00 0.06069 32600.00 31000.00 0.00 0.06136 32400.00 31200.00 0.00 0.06247 32600.00 31200.00 0.00 0.06307 32400.00 31400.00 0.00 0.06418 32600.00 31400.00 0.00 0.06466 32400.00 31600.00 0.00 0.06584 32600.00 31600.00 0.00 0.06620 32400.00 31800.00 0.00 0.06743 32600.00 31800.00 0.00 0.06764 32400.00 32000.00 0.00 0.06890 32400.00 32200.00 0.00 0.06999 32400.00 32400.00 0.00 0.07081 32400.00 32600.00 0.00 0.07090 32400.00 32600.00 5.00 0.07103 32400.00 32600.00 10.00 0.07115 32400.00 32600.00 15.00 0.07126 32400.00 32600.00 20.00 0.07134 32200.00 32600.00 25.00 0.07142 32200.00 32600.00 30.00 0.07153 32200.00 32600.00 35.00 0.07156 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 32283.86 32600.00 33.60 0.07166 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/008.ctf NX, NY, NZ: 512 512 1 MODE : real