CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/006.mrc Output diagnostic file name Micrographs_0601/006.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/006.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2500.000 8276.000 Mean, RMS : 4537.135 229.8179 TITLE 1: emi2mrc: 006.emi Sun Jan 05 12:33:16 2014 TITLE 2: kV 300, Mag 78000x, Def 29040, Spot 4, Int 55.10%, Obj 88.04%, Dif 73.67% TITLE 3: X 336.472, Y 277.988, Z -104.822, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01494 13400.00 10000.00 0.00 -0.01490 13600.00 10000.00 0.00 -0.01476 13800.00 10000.00 0.00 -0.01445 14000.00 10000.00 0.00 -0.01406 14200.00 10000.00 0.00 -0.01378 14400.00 10000.00 0.00 -0.01350 14600.00 10000.00 0.00 -0.01315 14800.00 10000.00 0.00 -0.01285 15000.00 10000.00 0.00 -0.01261 15200.00 10000.00 0.00 -0.01243 15400.00 10000.00 0.00 -0.01224 15600.00 10000.00 0.00 -0.01196 15800.00 10000.00 0.00 -0.01162 16000.00 10000.00 0.00 -0.01116 16200.00 10000.00 0.00 -0.01069 16400.00 10000.00 0.00 -0.01014 16600.00 10000.00 0.00 -0.00934 16800.00 10000.00 0.00 -0.00858 17000.00 10000.00 0.00 -0.00797 17200.00 10000.00 0.00 -0.00727 17400.00 10000.00 0.00 -0.00647 17600.00 10000.00 0.00 -0.00563 17800.00 10000.00 0.00 -0.00477 18000.00 10000.00 0.00 -0.00389 18200.00 10000.00 0.00 -0.00305 18400.00 10000.00 0.00 -0.00208 18600.00 10000.00 0.00 -0.00113 18800.00 10000.00 0.00 -0.00033 19000.00 10000.00 0.00 0.00044 19200.00 10000.00 0.00 0.00128 19400.00 10000.00 0.00 0.00222 19600.00 10000.00 0.00 0.00313 19800.00 10000.00 0.00 0.00395 20000.00 10000.00 0.00 0.00475 20200.00 10000.00 0.00 0.00560 20400.00 10000.00 0.00 0.00654 20600.00 10000.00 0.00 0.00757 20800.00 10000.00 0.00 0.00869 21000.00 10000.00 0.00 0.00988 21200.00 10000.00 0.00 0.01114 21400.00 10000.00 0.00 0.01236 21600.00 10000.00 0.00 0.01353 21800.00 10000.00 0.00 0.01468 22000.00 10000.00 0.00 0.01586 22200.00 10000.00 0.00 0.01720 22400.00 10000.00 0.00 0.01864 22600.00 10000.00 0.00 0.02007 22800.00 10000.00 0.00 0.02158 23000.00 10000.00 0.00 0.02308 23200.00 10000.00 0.00 0.02459 23400.00 10000.00 0.00 0.02623 23600.00 10000.00 0.00 0.02785 23800.00 10000.00 0.00 0.02960 24000.00 10000.00 0.00 0.03149 24200.00 10000.00 0.00 0.03307 24400.00 10000.00 0.00 0.03400 24600.00 10000.00 0.00 0.03445 24800.00 10000.00 0.00 0.03485 25000.00 10000.00 0.00 0.03505 24800.00 10200.00 0.00 0.03536 25000.00 10200.00 0.00 0.03551 24800.00 10400.00 0.00 0.03564 25000.00 10400.00 0.00 0.03578 24800.00 10600.00 0.00 0.03601 25000.00 10600.00 0.00 0.03615 24600.00 10800.00 0.00 0.03616 24800.00 10800.00 0.00 0.03647 25000.00 10800.00 0.00 0.03655 24600.00 11000.00 0.00 0.03662 24800.00 11000.00 0.00 0.03687 25000.00 11000.00 0.00 0.03691 24800.00 11200.00 0.00 0.03716 25000.00 11200.00 0.00 0.03718 24800.00 11400.00 0.00 0.03743 25000.00 11400.00 0.00 0.03743 24600.00 11600.00 0.00 0.03760 24800.00 11600.00 0.00 0.03783 24600.00 11800.00 0.00 0.03812 24800.00 11800.00 0.00 0.03829 24600.00 12000.00 0.00 0.03855 24800.00 12000.00 0.00 0.03871 25000.00 12000.00 0.00 0.03875 24600.00 12200.00 0.00 0.03903 24800.00 12200.00 0.00 0.03925 25000.00 12200.00 0.00 0.03935 24600.00 12400.00 0.00 0.03953 24800.00 12400.00 0.00 0.03979 25000.00 12400.00 0.00 0.03991 24800.00 12600.00 0.00 0.04019 25000.00 12600.00 0.00 0.04029 24600.00 12800.00 0.00 0.04038 24800.00 12800.00 0.00 0.04069 25000.00 12800.00 0.00 0.04079 24600.00 13000.00 0.00 0.04093 24800.00 13000.00 0.00 0.04127 25000.00 13000.00 0.00 0.04137 24600.00 13200.00 0.00 0.04148 24800.00 13200.00 0.00 0.04181 25000.00 13200.00 0.00 0.04192 24600.00 13400.00 0.00 0.04201 24800.00 13400.00 0.00 0.04235 25000.00 13400.00 0.00 0.04247 24600.00 13600.00 0.00 0.04268 24800.00 13600.00 0.00 0.04303 25000.00 13600.00 0.00 0.04318 24600.00 13800.00 0.00 0.04349 24800.00 13800.00 0.00 0.04383 25000.00 13800.00 0.00 0.04398 24600.00 14000.00 0.00 0.04426 24800.00 14000.00 0.00 0.04461 25000.00 14000.00 0.00 0.04474 24600.00 14200.00 0.00 0.04500 24800.00 14200.00 0.00 0.04535 25000.00 14200.00 0.00 0.04545 24600.00 14400.00 0.00 0.04573 24800.00 14400.00 0.00 0.04606 25000.00 14400.00 0.00 0.04611 24600.00 14600.00 0.00 0.04659 24800.00 14600.00 0.00 0.04690 25000.00 14600.00 0.00 0.04692 24400.00 14800.00 0.00 0.04694 24600.00 14800.00 0.00 0.04748 24800.00 14800.00 0.00 0.04777 25000.00 14800.00 0.00 0.04777 24600.00 15000.00 0.00 0.04829 24800.00 15000.00 0.00 0.04856 24600.00 15200.00 0.00 0.04906 24800.00 15200.00 0.00 0.04928 24400.00 15400.00 0.00 0.04941 24600.00 15400.00 0.00 0.04986 24800.00 15400.00 0.00 0.05006 24400.00 15600.00 0.00 0.05034 24600.00 15600.00 0.00 0.05077 24800.00 15600.00 0.00 0.05098 24400.00 15800.00 0.00 0.05128 24600.00 15800.00 0.00 0.05171 24800.00 15800.00 0.00 0.05194 24400.00 16000.00 0.00 0.05226 24600.00 16000.00 0.00 0.05272 24800.00 16000.00 0.00 0.05295 24400.00 16200.00 0.00 0.05332 24600.00 16200.00 0.00 0.05379 24800.00 16200.00 0.00 0.05402 24400.00 16400.00 0.00 0.05446 24600.00 16400.00 0.00 0.05492 24800.00 16400.00 0.00 0.05512 24400.00 16600.00 0.00 0.05553 24600.00 16600.00 0.00 0.05597 24800.00 16600.00 0.00 0.05615 24400.00 16800.00 0.00 0.05661 24600.00 16800.00 0.00 0.05703 24800.00 16800.00 0.00 0.05718 24400.00 17000.00 0.00 0.05770 24600.00 17000.00 0.00 0.05811 24800.00 17000.00 0.00 0.05825 24400.00 17200.00 0.00 0.05881 24600.00 17200.00 0.00 0.05923 24800.00 17200.00 0.00 0.05934 24400.00 17400.00 0.00 0.06004 24600.00 17400.00 0.00 0.06043 24800.00 17400.00 0.00 0.06050 24400.00 17600.00 0.00 0.06124 24600.00 17600.00 0.00 0.06157 24800.00 17600.00 0.00 0.06157 24400.00 17800.00 0.00 0.06243 24600.00 17800.00 0.00 0.06271 24400.00 18000.00 0.00 0.06356 24600.00 18000.00 0.00 0.06383 24400.00 18200.00 0.00 0.06480 24600.00 18200.00 0.00 0.06508 24200.00 18400.00 0.00 0.06510 24400.00 18400.00 0.00 0.06612 24600.00 18400.00 0.00 0.06640 24200.00 18600.00 0.00 0.06640 24400.00 18600.00 0.00 0.06743 24600.00 18600.00 0.00 0.06772 24200.00 18800.00 0.00 0.06784 24400.00 18800.00 0.00 0.06887 24600.00 18800.00 0.00 0.06912 24200.00 19000.00 0.00 0.06935 24400.00 19000.00 0.00 0.07034 24600.00 19000.00 0.00 0.07054 24200.00 19200.00 0.00 0.07086 24400.00 19200.00 0.00 0.07183 24600.00 19200.00 0.00 0.07204 24200.00 19400.00 0.00 0.07229 24400.00 19400.00 0.00 0.07330 24600.00 19400.00 0.00 0.07354 24200.00 19600.00 0.00 0.07381 24400.00 19600.00 0.00 0.07482 24600.00 19600.00 0.00 0.07502 24200.00 19800.00 0.00 0.07538 24400.00 19800.00 0.00 0.07635 24600.00 19800.00 0.00 0.07648 24200.00 20000.00 0.00 0.07703 24400.00 20000.00 0.00 0.07797 24600.00 20000.00 0.00 0.07805 24200.00 20200.00 0.00 0.07864 24400.00 20200.00 0.00 0.07955 24600.00 20200.00 0.00 0.07959 24200.00 20400.00 0.00 0.08020 24400.00 20400.00 0.00 0.08110 24200.00 20600.00 0.00 0.08186 24400.00 20600.00 0.00 0.08274 24200.00 20800.00 0.00 0.08355 24400.00 20800.00 0.00 0.08442 24200.00 21000.00 0.00 0.08551 24400.00 21000.00 0.00 0.08635 24200.00 21200.00 0.00 0.08747 24400.00 21200.00 0.00 0.08830 24200.00 21400.00 0.00 0.08951 24400.00 21400.00 0.00 0.09032 24200.00 21600.00 0.00 0.09154 24400.00 21600.00 0.00 0.09230 24200.00 21800.00 0.00 0.09358 24400.00 21800.00 0.00 0.09424 24200.00 22000.00 0.00 0.09574 24400.00 22000.00 0.00 0.09631 24200.00 22200.00 0.00 0.09791 24400.00 22200.00 0.00 0.09844 24200.00 22400.00 0.00 0.10048 24400.00 22400.00 0.00 0.10096 24000.00 22600.00 0.00 0.10097 24200.00 22600.00 0.00 0.10309 24400.00 22600.00 0.00 0.10348 24000.00 22800.00 0.00 0.10372 24200.00 22800.00 0.00 0.10581 24400.00 22800.00 0.00 0.10609 24000.00 23000.00 0.00 0.10645 24200.00 23000.00 0.00 0.10850 24400.00 23000.00 0.00 0.10858 24000.00 23200.00 0.00 0.10924 24200.00 23200.00 0.00 0.11117 24000.00 23400.00 0.00 0.11240 24200.00 23400.00 0.00 0.11413 24000.00 23600.00 0.00 0.11546 24200.00 23600.00 0.00 0.11692 24000.00 23800.00 0.00 0.11851 24200.00 23800.00 0.00 0.11955 24000.00 24000.00 0.00 0.12082 24200.00 24000.00 0.00 0.12139 24000.00 24200.00 0.00 0.12199 24000.00 24200.00 5.00 0.12219 24000.00 24200.00 10.00 0.12239 24000.00 24200.00 15.00 0.12257 24000.00 24200.00 20.00 0.12273 24000.00 24200.00 25.00 0.12285 24000.00 24200.00 30.00 0.12294 24000.00 24200.00 35.00 0.12298 24000.00 24200.00 40.00 0.12299 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23988.40 24287.00 37.78 0.12328 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/006.ctf NX, NY, NZ: 512 512 1 MODE : real