CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/005.mrc Output diagnostic file name Micrographs_0601/005.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/005.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2138.000 8666.000 Mean, RMS : 4541.459 222.8525 TITLE 1: emi2mrc: 005.emi Sun Jan 05 12:32:33 2014 TITLE 2: kV 300, Mag 78000x, Def 29040, Spot 4, Int 55.10%, Obj 88.04%, Dif 73.67% TITLE 3: X 333.885, Y 278.562, Z -104.822, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00776 14800.00 10000.00 0.00 -0.00755 15000.00 10000.00 0.00 -0.00723 15200.00 10000.00 0.00 -0.00698 15400.00 10000.00 0.00 -0.00675 15600.00 10000.00 0.00 -0.00636 15800.00 10000.00 0.00 -0.00576 16000.00 10000.00 0.00 -0.00514 16200.00 10000.00 0.00 -0.00465 16400.00 10000.00 0.00 -0.00420 16600.00 10000.00 0.00 -0.00383 16800.00 10000.00 0.00 -0.00354 17000.00 10000.00 0.00 -0.00316 17200.00 10000.00 0.00 -0.00262 17400.00 10000.00 0.00 -0.00201 17600.00 10000.00 0.00 -0.00137 17800.00 10000.00 0.00 -0.00069 18000.00 10000.00 0.00 0.00003 18200.00 10000.00 0.00 0.00074 18400.00 10000.00 0.00 0.00151 18600.00 10000.00 0.00 0.00227 18800.00 10000.00 0.00 0.00305 19000.00 10000.00 0.00 0.00398 19200.00 10000.00 0.00 0.00490 19400.00 10000.00 0.00 0.00571 19600.00 10000.00 0.00 0.00654 19800.00 10000.00 0.00 0.00744 20000.00 10000.00 0.00 0.00843 20200.00 10000.00 0.00 0.00944 20400.00 10000.00 0.00 0.01043 20600.00 10000.00 0.00 0.01148 20800.00 10000.00 0.00 0.01252 21000.00 10000.00 0.00 0.01350 21200.00 10000.00 0.00 0.01456 21400.00 10000.00 0.00 0.01562 21600.00 10000.00 0.00 0.01660 21800.00 10000.00 0.00 0.01756 22000.00 10000.00 0.00 0.01862 22200.00 10000.00 0.00 0.01973 22400.00 10000.00 0.00 0.02079 22600.00 10000.00 0.00 0.02194 22800.00 10000.00 0.00 0.02323 23000.00 10000.00 0.00 0.02461 23200.00 10000.00 0.00 0.02597 23400.00 10000.00 0.00 0.02719 23600.00 10000.00 0.00 0.02848 23800.00 10000.00 0.00 0.02992 24000.00 10000.00 0.00 0.03142 24200.00 10000.00 0.00 0.03307 24400.00 10000.00 0.00 0.03483 24600.00 10000.00 0.00 0.03659 24800.00 10000.00 0.00 0.03807 25000.00 10000.00 0.00 0.03901 25200.00 10000.00 0.00 0.03951 25400.00 10000.00 0.00 0.03967 25600.00 10000.00 0.00 0.03968 25200.00 10200.00 0.00 0.03990 25400.00 10200.00 0.00 0.04007 25600.00 10200.00 0.00 0.04007 25200.00 10400.00 0.00 0.04038 25400.00 10400.00 0.00 0.04053 25200.00 10600.00 0.00 0.04085 25400.00 10600.00 0.00 0.04098 25200.00 10800.00 0.00 0.04143 25400.00 10800.00 0.00 0.04156 25000.00 11000.00 0.00 0.04160 25200.00 11000.00 0.00 0.04208 25400.00 11000.00 0.00 0.04218 25200.00 11200.00 0.00 0.04249 25400.00 11200.00 0.00 0.04253 25200.00 11400.00 0.00 0.04287 25400.00 11400.00 0.00 0.04291 25200.00 11600.00 0.00 0.04325 25400.00 11600.00 0.00 0.04333 25200.00 11800.00 0.00 0.04368 25400.00 11800.00 0.00 0.04379 25000.00 12000.00 0.00 0.04381 25200.00 12000.00 0.00 0.04423 25400.00 12000.00 0.00 0.04433 25000.00 12200.00 0.00 0.04440 25200.00 12200.00 0.00 0.04480 25400.00 12200.00 0.00 0.04486 25000.00 12400.00 0.00 0.04499 25200.00 12400.00 0.00 0.04540 25400.00 12400.00 0.00 0.04543 25000.00 12600.00 0.00 0.04563 25200.00 12600.00 0.00 0.04605 25400.00 12600.00 0.00 0.04605 25000.00 12800.00 0.00 0.04633 25200.00 12800.00 0.00 0.04672 25000.00 13000.00 0.00 0.04689 25200.00 13000.00 0.00 0.04724 25000.00 13200.00 0.00 0.04743 25200.00 13200.00 0.00 0.04776 25000.00 13400.00 0.00 0.04800 25200.00 13400.00 0.00 0.04828 25000.00 13600.00 0.00 0.04858 25200.00 13600.00 0.00 0.04879 25000.00 13800.00 0.00 0.04913 25200.00 13800.00 0.00 0.04934 25000.00 14000.00 0.00 0.04964 25200.00 14000.00 0.00 0.04984 25000.00 14200.00 0.00 0.05023 25200.00 14200.00 0.00 0.05042 25000.00 14400.00 0.00 0.05079 25200.00 14400.00 0.00 0.05098 25000.00 14600.00 0.00 0.05128 25200.00 14600.00 0.00 0.05147 25000.00 14800.00 0.00 0.05184 25200.00 14800.00 0.00 0.05206 25000.00 15000.00 0.00 0.05250 25200.00 15000.00 0.00 0.05275 25000.00 15200.00 0.00 0.05313 25200.00 15200.00 0.00 0.05343 25000.00 15400.00 0.00 0.05391 25200.00 15400.00 0.00 0.05424 25000.00 15600.00 0.00 0.05472 25200.00 15600.00 0.00 0.05507 25000.00 15800.00 0.00 0.05554 25200.00 15800.00 0.00 0.05590 25000.00 16000.00 0.00 0.05665 25200.00 16000.00 0.00 0.05700 25000.00 16200.00 0.00 0.05764 25200.00 16200.00 0.00 0.05794 25000.00 16400.00 0.00 0.05852 25200.00 16400.00 0.00 0.05879 25000.00 16600.00 0.00 0.05953 25200.00 16600.00 0.00 0.05981 25000.00 16800.00 0.00 0.06061 25200.00 16800.00 0.00 0.06086 25000.00 17000.00 0.00 0.06162 25200.00 17000.00 0.00 0.06182 25000.00 17200.00 0.00 0.06262 25200.00 17200.00 0.00 0.06281 24800.00 17400.00 0.00 0.06288 25000.00 17400.00 0.00 0.06369 25200.00 17400.00 0.00 0.06387 24800.00 17600.00 0.00 0.06391 25000.00 17600.00 0.00 0.06470 25200.00 17600.00 0.00 0.06486 24800.00 17800.00 0.00 0.06500 25000.00 17800.00 0.00 0.06580 25200.00 17800.00 0.00 0.06597 24800.00 18000.00 0.00 0.06610 25000.00 18000.00 0.00 0.06694 25200.00 18000.00 0.00 0.06714 24800.00 18200.00 0.00 0.06739 25000.00 18200.00 0.00 0.06823 25200.00 18200.00 0.00 0.06840 24800.00 18400.00 0.00 0.06877 25000.00 18400.00 0.00 0.06957 25200.00 18400.00 0.00 0.06967 24800.00 18600.00 0.00 0.07014 25000.00 18600.00 0.00 0.07088 25200.00 18600.00 0.00 0.07094 24800.00 18800.00 0.00 0.07154 25000.00 18800.00 0.00 0.07224 25200.00 18800.00 0.00 0.07229 24800.00 19000.00 0.00 0.07287 25000.00 19000.00 0.00 0.07357 25200.00 19000.00 0.00 0.07362 24800.00 19200.00 0.00 0.07432 25000.00 19200.00 0.00 0.07498 25200.00 19200.00 0.00 0.07500 24800.00 19400.00 0.00 0.07564 25000.00 19400.00 0.00 0.07625 24800.00 19600.00 0.00 0.07691 25000.00 19600.00 0.00 0.07750 24800.00 19800.00 0.00 0.07817 25000.00 19800.00 0.00 0.07876 24800.00 20000.00 0.00 0.07947 25000.00 20000.00 0.00 0.08006 25200.00 20000.00 0.00 0.08006 24800.00 20200.00 0.00 0.08094 25000.00 20200.00 0.00 0.08157 25200.00 20200.00 0.00 0.08161 24800.00 20400.00 0.00 0.08249 25000.00 20400.00 0.00 0.08317 25200.00 20400.00 0.00 0.08322 24800.00 20600.00 0.00 0.08420 25000.00 20600.00 0.00 0.08492 25200.00 20600.00 0.00 0.08493 24800.00 20800.00 0.00 0.08588 25000.00 20800.00 0.00 0.08659 24800.00 21000.00 0.00 0.08756 25000.00 21000.00 0.00 0.08825 24800.00 21200.00 0.00 0.08935 25000.00 21200.00 0.00 0.09003 24800.00 21400.00 0.00 0.09134 25000.00 21400.00 0.00 0.09201 24800.00 21600.00 0.00 0.09334 25000.00 21600.00 0.00 0.09396 24800.00 21800.00 0.00 0.09526 25000.00 21800.00 0.00 0.09581 24800.00 22000.00 0.00 0.09722 25000.00 22000.00 0.00 0.09775 24800.00 22200.00 0.00 0.09926 25000.00 22200.00 0.00 0.09971 24800.00 22400.00 0.00 0.10149 25000.00 22400.00 0.00 0.10179 24600.00 22600.00 0.00 0.10193 24800.00 22600.00 0.00 0.10366 25000.00 22600.00 0.00 0.10380 24600.00 22800.00 0.00 0.10427 24800.00 22800.00 0.00 0.10592 24600.00 23000.00 0.00 0.10656 24800.00 23000.00 0.00 0.10810 24600.00 23200.00 0.00 0.10886 24800.00 23200.00 0.00 0.11027 24600.00 23400.00 0.00 0.11132 24800.00 23400.00 0.00 0.11263 24600.00 23600.00 0.00 0.11385 24800.00 23600.00 0.00 0.11506 24600.00 23800.00 0.00 0.11656 24800.00 23800.00 0.00 0.11759 24600.00 24000.00 0.00 0.11911 24800.00 24000.00 0.00 0.11990 24600.00 24200.00 0.00 0.12175 24800.00 24200.00 0.00 0.12222 24600.00 24400.00 0.00 0.12391 24800.00 24400.00 0.00 0.12401 24400.00 24600.00 0.00 0.12411 24600.00 24600.00 0.00 0.12563 24600.00 24800.00 0.00 0.12607 24600.00 24800.00 5.00 0.12628 24600.00 24800.00 10.00 0.12647 24600.00 24800.00 15.00 0.12664 24600.00 24800.00 20.00 0.12679 24600.00 24800.00 25.00 0.12690 24600.00 24800.00 30.00 0.12697 24600.00 24800.00 35.00 0.12701 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24544.03 24847.09 35.65 0.12714 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/005.ctf NX, NY, NZ: 512 512 1 MODE : real