CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/004.mrc Output diagnostic file name Micrographs_0601/004.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/004.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2468.000 7888.000 Mean, RMS : 4540.982 224.0669 TITLE 1: emi2mrc: 004.emi Sun Jan 05 12:31:47 2014 TITLE 2: kV 300, Mag 78000x, Def 29040, Spot 4, Int 55.10%, Obj 88.04%, Dif 73.67% TITLE 3: X 331.399, Y 278.718, Z -104.822, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00709 10200.00 10000.00 0.00 -0.00696 10400.00 10000.00 0.00 -0.00694 12000.00 10000.00 0.00 -0.00682 12200.00 10000.00 0.00 -0.00659 12400.00 10000.00 0.00 -0.00637 12600.00 10000.00 0.00 -0.00616 12800.00 10000.00 0.00 -0.00606 13000.00 10000.00 0.00 -0.00596 13200.00 10000.00 0.00 -0.00580 13400.00 10000.00 0.00 -0.00564 13600.00 10000.00 0.00 -0.00553 13800.00 10000.00 0.00 -0.00551 14000.00 10000.00 0.00 -0.00546 14200.00 10000.00 0.00 -0.00534 14400.00 10000.00 0.00 -0.00524 14600.00 10000.00 0.00 -0.00514 14800.00 10000.00 0.00 -0.00484 15000.00 10000.00 0.00 -0.00428 15200.00 10000.00 0.00 -0.00372 15400.00 10000.00 0.00 -0.00326 15600.00 10000.00 0.00 -0.00270 15800.00 10000.00 0.00 -0.00204 16000.00 10000.00 0.00 -0.00141 16200.00 10000.00 0.00 -0.00087 16400.00 10000.00 0.00 -0.00038 16600.00 10000.00 0.00 0.00016 16800.00 10000.00 0.00 0.00072 17000.00 10000.00 0.00 0.00129 17200.00 10000.00 0.00 0.00189 17400.00 10000.00 0.00 0.00260 17600.00 10000.00 0.00 0.00346 17800.00 10000.00 0.00 0.00424 18000.00 10000.00 0.00 0.00500 18200.00 10000.00 0.00 0.00588 18400.00 10000.00 0.00 0.00670 18600.00 10000.00 0.00 0.00748 18800.00 10000.00 0.00 0.00833 19000.00 10000.00 0.00 0.00926 19200.00 10000.00 0.00 0.01022 19400.00 10000.00 0.00 0.01122 19600.00 10000.00 0.00 0.01227 19800.00 10000.00 0.00 0.01323 20000.00 10000.00 0.00 0.01419 20200.00 10000.00 0.00 0.01524 20400.00 10000.00 0.00 0.01615 20600.00 10000.00 0.00 0.01710 20800.00 10000.00 0.00 0.01810 21000.00 10000.00 0.00 0.01904 21200.00 10000.00 0.00 0.01999 21400.00 10000.00 0.00 0.02099 21600.00 10000.00 0.00 0.02214 21800.00 10000.00 0.00 0.02337 22000.00 10000.00 0.00 0.02465 22200.00 10000.00 0.00 0.02595 22400.00 10000.00 0.00 0.02737 22600.00 10000.00 0.00 0.02895 22800.00 10000.00 0.00 0.03047 23000.00 10000.00 0.00 0.03213 23200.00 10000.00 0.00 0.03383 23400.00 10000.00 0.00 0.03556 23600.00 10000.00 0.00 0.03733 23800.00 10000.00 0.00 0.03897 24000.00 10000.00 0.00 0.04058 24200.00 10000.00 0.00 0.04206 24400.00 10000.00 0.00 0.04319 24600.00 10000.00 0.00 0.04391 24800.00 10000.00 0.00 0.04430 25000.00 10000.00 0.00 0.04444 24800.00 10200.00 0.00 0.04472 25000.00 10200.00 0.00 0.04486 24800.00 10400.00 0.00 0.04509 25000.00 10400.00 0.00 0.04520 24800.00 10600.00 0.00 0.04544 25000.00 10600.00 0.00 0.04553 24800.00 10800.00 0.00 0.04580 25000.00 10800.00 0.00 0.04588 24800.00 11000.00 0.00 0.04626 25000.00 11000.00 0.00 0.04632 24800.00 11200.00 0.00 0.04666 25000.00 11200.00 0.00 0.04669 24800.00 11400.00 0.00 0.04699 25000.00 11400.00 0.00 0.04704 24800.00 11600.00 0.00 0.04737 25000.00 11600.00 0.00 0.04747 24800.00 11800.00 0.00 0.04778 25000.00 11800.00 0.00 0.04791 24800.00 12000.00 0.00 0.04824 25000.00 12000.00 0.00 0.04836 24600.00 12200.00 0.00 0.04842 24800.00 12200.00 0.00 0.04879 25000.00 12200.00 0.00 0.04888 24800.00 12400.00 0.00 0.04922 25000.00 12400.00 0.00 0.04928 24600.00 12600.00 0.00 0.04931 24800.00 12600.00 0.00 0.04964 25000.00 12600.00 0.00 0.04967 24600.00 12800.00 0.00 0.04986 24800.00 12800.00 0.00 0.05015 25000.00 12800.00 0.00 0.05018 24600.00 13000.00 0.00 0.05031 24800.00 13000.00 0.00 0.05058 25000.00 13000.00 0.00 0.05060 24600.00 13200.00 0.00 0.05079 24800.00 13200.00 0.00 0.05109 25000.00 13200.00 0.00 0.05115 24600.00 13400.00 0.00 0.05139 24800.00 13400.00 0.00 0.05173 25000.00 13400.00 0.00 0.05180 24600.00 13600.00 0.00 0.05220 24800.00 13600.00 0.00 0.05253 25000.00 13600.00 0.00 0.05256 24600.00 13800.00 0.00 0.05293 24800.00 13800.00 0.00 0.05322 24600.00 14000.00 0.00 0.05349 24800.00 14000.00 0.00 0.05376 24600.00 14200.00 0.00 0.05418 24800.00 14200.00 0.00 0.05443 24600.00 14400.00 0.00 0.05491 24800.00 14400.00 0.00 0.05512 24600.00 14600.00 0.00 0.05574 24800.00 14600.00 0.00 0.05592 24600.00 14800.00 0.00 0.05648 24800.00 14800.00 0.00 0.05661 24600.00 15000.00 0.00 0.05713 24800.00 15000.00 0.00 0.05726 24400.00 15200.00 0.00 0.05738 24600.00 15200.00 0.00 0.05793 24800.00 15200.00 0.00 0.05811 24400.00 15400.00 0.00 0.05824 24600.00 15400.00 0.00 0.05883 24800.00 15400.00 0.00 0.05904 24400.00 15600.00 0.00 0.05917 24600.00 15600.00 0.00 0.05978 24800.00 15600.00 0.00 0.06000 24400.00 15800.00 0.00 0.06020 24600.00 15800.00 0.00 0.06081 24800.00 15800.00 0.00 0.06102 24400.00 16000.00 0.00 0.06125 24600.00 16000.00 0.00 0.06182 24800.00 16000.00 0.00 0.06202 24400.00 16200.00 0.00 0.06213 24600.00 16200.00 0.00 0.06268 24800.00 16200.00 0.00 0.06289 24400.00 16400.00 0.00 0.06310 24600.00 16400.00 0.00 0.06366 24800.00 16400.00 0.00 0.06389 24400.00 16600.00 0.00 0.06411 24600.00 16600.00 0.00 0.06469 24800.00 16600.00 0.00 0.06493 24400.00 16800.00 0.00 0.06520 24600.00 16800.00 0.00 0.06583 24800.00 16800.00 0.00 0.06608 24400.00 17000.00 0.00 0.06637 24600.00 17000.00 0.00 0.06704 24800.00 17000.00 0.00 0.06727 24400.00 17200.00 0.00 0.06756 24600.00 17200.00 0.00 0.06821 24800.00 17200.00 0.00 0.06843 24400.00 17400.00 0.00 0.06881 24600.00 17400.00 0.00 0.06946 24800.00 17400.00 0.00 0.06965 24400.00 17600.00 0.00 0.06994 24600.00 17600.00 0.00 0.07061 24800.00 17600.00 0.00 0.07079 24400.00 17800.00 0.00 0.07126 24600.00 17800.00 0.00 0.07196 24800.00 17800.00 0.00 0.07210 24400.00 18000.00 0.00 0.07268 24600.00 18000.00 0.00 0.07331 24800.00 18000.00 0.00 0.07339 24400.00 18200.00 0.00 0.07398 24600.00 18200.00 0.00 0.07456 24800.00 18200.00 0.00 0.07461 24400.00 18400.00 0.00 0.07531 24600.00 18400.00 0.00 0.07589 24800.00 18400.00 0.00 0.07596 24400.00 18600.00 0.00 0.07677 24600.00 18600.00 0.00 0.07731 24800.00 18600.00 0.00 0.07734 24400.00 18800.00 0.00 0.07826 24600.00 18800.00 0.00 0.07874 24400.00 19000.00 0.00 0.07960 24600.00 19000.00 0.00 0.08004 24400.00 19200.00 0.00 0.08099 24600.00 19200.00 0.00 0.08141 24400.00 19400.00 0.00 0.08240 24600.00 19400.00 0.00 0.08281 24400.00 19600.00 0.00 0.08399 24600.00 19600.00 0.00 0.08437 24400.00 19800.00 0.00 0.08556 24600.00 19800.00 0.00 0.08592 24400.00 20000.00 0.00 0.08711 24600.00 20000.00 0.00 0.08744 24400.00 20200.00 0.00 0.08866 24600.00 20200.00 0.00 0.08894 24400.00 20400.00 0.00 0.09015 24600.00 20400.00 0.00 0.09041 24200.00 20600.00 0.00 0.09066 24400.00 20600.00 0.00 0.09193 24600.00 20600.00 0.00 0.09218 24200.00 20800.00 0.00 0.09248 24400.00 20800.00 0.00 0.09376 24600.00 20800.00 0.00 0.09394 24200.00 21000.00 0.00 0.09450 24400.00 21000.00 0.00 0.09573 24600.00 21000.00 0.00 0.09581 24200.00 21200.00 0.00 0.09645 24400.00 21200.00 0.00 0.09762 24200.00 21400.00 0.00 0.09831 24400.00 21400.00 0.00 0.09940 24200.00 21600.00 0.00 0.10020 24400.00 21600.00 0.00 0.10122 24200.00 21800.00 0.00 0.10206 24400.00 21800.00 0.00 0.10302 24200.00 22000.00 0.00 0.10418 24400.00 22000.00 0.00 0.10512 24200.00 22200.00 0.00 0.10634 24400.00 22200.00 0.00 0.10722 24200.00 22400.00 0.00 0.10881 24400.00 22400.00 0.00 0.10958 24200.00 22600.00 0.00 0.11139 24400.00 22600.00 0.00 0.11199 24000.00 22800.00 0.00 0.11217 24200.00 22800.00 0.00 0.11388 24400.00 22800.00 0.00 0.11434 24000.00 23000.00 0.00 0.11476 24200.00 23000.00 0.00 0.11640 24400.00 23000.00 0.00 0.11674 24000.00 23200.00 0.00 0.11750 24200.00 23200.00 0.00 0.11903 24400.00 23200.00 0.00 0.11918 24000.00 23400.00 0.00 0.12033 24200.00 23400.00 0.00 0.12168 24000.00 23600.00 0.00 0.12301 24200.00 23600.00 0.00 0.12416 24000.00 23800.00 0.00 0.12590 24200.00 23800.00 0.00 0.12670 24000.00 24000.00 0.00 0.12783 24200.00 24000.00 0.00 0.12830 24000.00 24200.00 0.00 0.12848 24200.00 24200.00 0.00 0.12860 24000.00 24200.00 10.00 0.12872 24000.00 24200.00 15.00 0.12883 24000.00 24200.00 20.00 0.12894 24000.00 24200.00 25.00 0.12902 24000.00 24200.00 30.00 0.12909 24000.00 24200.00 35.00 0.12914 24000.00 24200.00 40.00 0.12916 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23966.63 24297.32 42.34 0.12939 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/004.ctf NX, NY, NZ: 512 512 1 MODE : real